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Volumn 1669, Issue 2, 2005, Pages 193-207

New effects in polynucleotide release from cationic lipid carriers revealed by confocal imaging, fluorescence cross-correlation spectroscopy and single particle tracking

Author keywords

Endosomal membrane; Fluorescence microscopy; Giant unilamellar vesicles; Lipoplex; Release mechanism; Single particle tracking

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; DIOLEOYLPHOSPHATIDYLCHOLINE; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; DIOLEOYLPHOSPHATIDYLSERINE; DIOLEOYLTRIMETHYLAMMONIUM PROPANE; DIPALMITOYLPHOSPHATIDYLSERINE; DOUBLE STRANDED DNA; LIPID; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; POLYNUCLEOTIDE; SPHINGOMYELIN; UNCLASSIFIED DRUG;

EID: 18944397052     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2005.02.011     Document Type: Article
Times cited : (22)

References (63)
  • 1
    • 1642362625 scopus 로고    scopus 로고
    • Drug delivery systems: Entering the mainstream
    • T.M. Allen, and P.R. Cullis Drug delivery systems: entering the mainstream Science 303 2004 1818 1822
    • (2004) Science , vol.303 , pp. 1818-1822
    • Allen, T.M.1    Cullis, P.R.2
  • 2
    • 0348014774 scopus 로고    scopus 로고
    • Approaches for the sequence specific knockdown of mRNA
    • L. Scherer, and J. Rossi Approaches for the sequence specific knockdown of mRNA Nat. Biotechnol. 21 2003 1457 1465
    • (2003) Nat. Biotechnol. , vol.21 , pp. 1457-1465
    • Scherer, L.1    Rossi, J.2
  • 3
    • 0036555163 scopus 로고    scopus 로고
    • Biophysical aspects of using liposomes as delivery vehicles
    • A.S. Ulrich Biophysical aspects of using liposomes as delivery vehicles Biosci. Rep. 22 2002 129 150
    • (2002) Biosci. Rep. , vol.22 , pp. 129-150
    • Ulrich, A.S.1
  • 4
    • 0035946624 scopus 로고    scopus 로고
    • Cationic lipid-DNA complexes in gene delivery: From biophysics to biological applications
    • M.C. Pedroso de Lima, S. Simoes, P. Pires, H. Faneca, and N. Duzgunes Cationic lipid-DNA complexes in gene delivery: from biophysics to biological applications Adv. Drug Deliv. Rev. 47 2001 277 294
    • (2001) Adv. Drug Deliv. Rev. , vol.47 , pp. 277-294
    • Pedroso De Lima, M.C.1    Simoes, S.2    Pires, P.3    Faneca, H.4    Duzgunes, N.5
  • 5
    • 0033902946 scopus 로고    scopus 로고
    • Structure and function of lipid-DNA complexes for gene delivery
    • S. Chesnoy, and L. Huang Structure and function of lipid-DNA complexes for gene delivery Annu. Rev. Biophys. Biomol. Struct. 2 2000 27 47
    • (2000) Annu. Rev. Biophys. Biomol. Struct. , vol.2 , pp. 27-47
    • Chesnoy, S.1    Huang, L.2
  • 8
    • 0027179724 scopus 로고
    • Mode of formation and structural features of DNA-cationic liposome complexes used for transfection
    • H. Gershon, R. Ghirlando, S.B. Guttman, and A. Minsky Mode of formation and structural features of DNA-cationic liposome complexes used for transfection Biochemistry 32 1993 7143 7151
    • (1993) Biochemistry , vol.32 , pp. 7143-7151
    • Gershon, H.1    Ghirlando, R.2    Guttman, S.B.3    Minsky, A.4
  • 9
    • 0029920009 scopus 로고    scopus 로고
    • Cationic lipid binding to DNA: Characterization of complex formation
    • F.M.P. Wong, D.L Reimer, and M.B. Bally Cationic lipid binding to DNA: characterization of complex formation Biochemistry 35 1996 5763 5776
    • (1996) Biochemistry , vol.35 , pp. 5763-5776
    • Wong, F.M.P.1    Reimer, D.L.2    Bally, M.B.3
  • 12
    • 0032479572 scopus 로고    scopus 로고
    • An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery
    • I. Koltover, T. Salditt, J.O. Raedler, and C.R. Safinya An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery Science 281 1998 78 81
    • (1998) Science , vol.281 , pp. 78-81
    • Koltover, I.1    Salditt, T.2    Raedler, J.O.3    Safinya, C.R.4
  • 13
    • 0032805569 scopus 로고    scopus 로고
    • Phase diagram, stability, and overcharging of lamellar cationic lipid-DNA self-assembled complexes
    • I. Koltover, T. Salditt, and C.R. Safinya Phase diagram, stability, and overcharging of lamellar cationic lipid-DNA self-assembled complexes Biophys. J. 77 1999 915 924
    • (1999) Biophys. J. , vol.77 , pp. 915-924
    • Koltover, I.1    Salditt, T.2    Safinya, C.R.3
  • 14
    • 0033050727 scopus 로고    scopus 로고
    • Physicochemical characterization and purification of cationic lipoplexes
    • Y. Xu, S.W. Hui, P. Frederik, and F.C. Szoka Physicochemical characterization and purification of cationic lipoplexes Biophys. J. 77 1999 341 353
    • (1999) Biophys. J. , vol.77 , pp. 341-353
    • Xu, Y.1    Hui, S.W.2    Frederik, P.3    Szoka, F.C.4
  • 15
    • 0033863232 scopus 로고    scopus 로고
    • Lipoplex formation under equilibrium conditions reveals a three-step mechanism
    • V. Oberle, U. Bakowsky, I.S. Zuhorn, and D. Hoekstra Lipoplex formation under equilibrium conditions reveals a three-step mechanism Biophys. J. 79 2000 1447 1454
    • (2000) Biophys. J. , vol.79 , pp. 1447-1454
    • Oberle, V.1    Bakowsky, U.2    Zuhorn, I.S.3    Hoekstra, D.4
  • 17
    • 0035312248 scopus 로고    scopus 로고
    • Trends in lipoplex physical properties dependent on cationic lipid structure, vehicle and complexation procedure do not correlate with biological activity
    • M.E. Ferrari, D. Rusalov, J. Enas, and C.J. Wheeler Trends in lipoplex physical properties dependent on cationic lipid structure, vehicle and complexation procedure do not correlate with biological activity Nucleic Acids Res. 29 2001 1539 1548
    • (2001) Nucleic Acids Res. , vol.29 , pp. 1539-1548
    • Ferrari, M.E.1    Rusalov, D.2    Enas, J.3    Wheeler, C.J.4
  • 19
    • 0035861658 scopus 로고    scopus 로고
    • Molecular shape of the cationic lipid controls the structure of cationic lipid/dioleoylphosphatidylethanolamine-DNA complexes and the efficiency of gene delivery
    • J. Smisterová, A. Wagenaar, M.C.A. Stuart, E. Polushkin, G. Brinke, R. Hulst, J.B.F.N. Engberts, and D. Hoekstra Molecular shape of the cationic lipid controls the structure of cationic lipid/ dioleoylphosphatidylethanolamine-DNA complexes and the efficiency of gene delivery J. Biol. Chem. 276 2001 47615 47622
    • (2001) J. Biol. Chem. , vol.276 , pp. 47615-47622
    • Smisterová, J.1    Wagenaar, A.2    Stuart, M.C.A.3    Polushkin, E.4    Brinke, G.5    Hulst, R.6    Engberts, J.B.F.N.7    Hoekstra, D.8
  • 20
    • 0242353819 scopus 로고    scopus 로고
    • Characterization of DNA/Lipid complexes by fluorescence resonance energy transfer
    • C. Madeira, L.M.S. Loura, R.M. Aires-Barros, A. Fedorov, and M. Prieto Characterization of DNA/Lipid complexes by fluorescence resonance energy transfer Biophys. J. 85 2003 3106 3119
    • (2003) Biophys. J. , vol.85 , pp. 3106-3119
    • Madeira, C.1    Loura, L.M.S.2    Aires-Barros, R.M.3    Fedorov, A.4    Prieto, M.5
  • 21
    • 0037622183 scopus 로고    scopus 로고
    • Three-dimensional imaging of lipid gene-carriers: Membrane charge density controls universal transfection behavior in lamellar cationic liposome-DNA complexes
    • A.J. Lin, N.L. Slack, A. Ahmad, C.X. George, C.E. Samuel, and C.R. Safinya Three-dimensional imaging of lipid gene-carriers: membrane charge density controls universal transfection behavior in lamellar cationic liposome-DNA complexes Biophys. J. 84 2003 3307 3316
    • (2003) Biophys. J. , vol.84 , pp. 3307-3316
    • Lin, A.J.1    Slack, N.L.2    Ahmad, A.3    George, C.X.4    Samuel, C.E.5    Safinya, C.R.6
  • 23
    • 0028097638 scopus 로고
    • DNA transfection mediated by cationic liposomes containing lipopolylysine: Characterization and mechanism of action
    • X. Zhou, and L. Huang DNA transfection mediated by cationic liposomes containing lipopolylysine: characterization and mechanism of action Biochim. Biophys. Acta 1189 1994 195 203
    • (1994) Biochim. Biophys. Acta , vol.1189 , pp. 195-203
    • Zhou, X.1    Huang, L.2
  • 24
    • 0029019120 scopus 로고
    • Fusion of cationic liposomes with mammalian cells occurs after endocytosis
    • I. Wrobel, and D. Collins Fusion of cationic liposomes with mammalian cells occurs after endocytosis Biochim. Biophys. Acta 1235 1995 296 304
    • (1995) Biochim. Biophys. Acta , vol.1235 , pp. 296-304
    • Wrobel, I.1    Collins, D.2
  • 25
    • 0030071910 scopus 로고    scopus 로고
    • Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes
    • D.S. Friend, D. Papahadjopoulos, and R.J. Debs Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes Biochim. Biophys. Acta 1278 1996 41 50
    • (1996) Biochim. Biophys. Acta , vol.1278 , pp. 41-50
    • Friend, D.S.1    Papahadjopoulos, D.2    Debs, R.J.3
  • 26
    • 0029856329 scopus 로고    scopus 로고
    • Intracellular distribution and mechanism of delivery of oligonucleotides mediated by cationic lipids
    • O. Zelphati, and F.C. Szoka Intracellular distribution and mechanism of delivery of oligonucleotides mediated by cationic lipids Pharm. Res. 13 1996 1367 1372
    • (1996) Pharm. Res. , vol.13 , pp. 1367-1372
    • Zelphati, O.1    Szoka, F.C.2
  • 27
    • 0037124051 scopus 로고    scopus 로고
    • Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis
    • I.S. Zuhorn, R. Kalicharan, and D. Hoekstra Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis J. Biol. Chem. 277 2002 18021 18028
    • (2002) J. Biol. Chem. , vol.277 , pp. 18021-18028
    • Zuhorn, I.S.1    Kalicharan, R.2    Hoekstra, D.3
  • 28
    • 0036781643 scopus 로고    scopus 로고
    • On the mechanism of cationic amphiphile-mediated transfection. to fuse or not to fuse: Is that the question?
    • I.S. Zuhorn, and D. Hoekstra On the mechanism of cationic amphiphile-mediated transfection. To fuse or not to fuse: is that the question? J. Membr. Biol. 189 2002 167 179
    • (2002) J. Membr. Biol. , vol.189 , pp. 167-179
    • Zuhorn, I.S.1    Hoekstra, D.2
  • 29
    • 0024269716 scopus 로고
    • Endosomes differ from plasma membrane in the phospholipid molecular species composition
    • R. Urade, Y. Hayashi, and M. Kito Endosomes differ from plasma membrane in the phospholipid molecular species composition Biochim. Biophys. Acta 946 1988 151 163
    • (1988) Biochim. Biophys. Acta , vol.946 , pp. 151-163
    • Urade, R.1    Hayashi, Y.2    Kito, M.3
  • 31
    • 0029964866 scopus 로고    scopus 로고
    • Mechanism of oligonucleotide release from cationic liposomes
    • O. Zelphati, and F.C. Szoka Mechanism of oligonucleotide release from cationic liposomes Proc. Natl. Acad. Sci. U. S. A. 93 1996 11493 11498
    • (1996) Proc. Natl. Acad. Sci. U. S. A. , vol.93 , pp. 11493-11498
    • Zelphati, O.1    Szoka, F.C.2
  • 32
    • 0029949266 scopus 로고    scopus 로고
    • Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection
    • Y. Xu, and F.C. Szoka Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection Biochemistry 35 1996 5616 5623
    • (1996) Biochemistry , vol.35 , pp. 5616-5623
    • Xu, Y.1    Szoka, F.C.2
  • 33
    • 0030968915 scopus 로고    scopus 로고
    • Drug-induced transmembrane lipid scrambling in erythrocytes and in liposomes requires the presence of polyanionic phospholipids
    • C. Moreau, J.C. Sulpice, P.F. Devaux, and A. Zachowski Drug-induced transmembrane lipid scrambling in erythrocytes and in liposomes requires the presence of polyanionic phospholipids Mol. Membr. Biol. 14 1997 5 12
    • (1997) Mol. Membr. Biol. , vol.14 , pp. 5-12
    • Moreau, C.1    Sulpice, J.C.2    Devaux, P.F.3    Zachowski, A.4
  • 34
    • 0001505065 scopus 로고
    • ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane; Relation to shape change
    • M. Seigneuret, and P.F. Devaux ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane; relation to shape change Proc. Natl. Acad. Sci. U. S. A. 81 1984 3751 3755
    • (1984) Proc. Natl. Acad. Sci. U. S. A. , vol.81 , pp. 3751-3755
    • Seigneuret, M.1    Devaux, P.F.2
  • 35
    • 0022371311 scopus 로고
    • Incorporation and translocation of aminophospholipids in human erythrocytes
    • D.L. Daleke, and W.H. Huestis Incorporation and translocation of aminophospholipids in human erythrocytes Biochemistry 24 1985 5406 5416
    • (1985) Biochemistry , vol.24 , pp. 5406-5416
    • Daleke, D.L.1    Huestis, W.H.2
  • 37
    • 0025876649 scopus 로고
    • Static and dynamic lipid asymmetry in cell membranes
    • P.F. Devaux Static and dynamic lipid asymmetry in cell membranes Biochemistry 30 1991 1163 1173
    • (1991) Biochemistry , vol.30 , pp. 1163-1173
    • Devaux, P.F.1
  • 38
    • 0029964587 scopus 로고    scopus 로고
    • Transbilayer movement of fluorescent and spinlabeled phospholipids in the plasma membrane of human fibroblasts: A quantitative approach
    • T. Pomorski, P. Müller, B. Zimmermann, K. Burger, P.F. Devaux, and A. Herrmann Transbilayer movement of fluorescent and spinlabeled phospholipids in the plasma membrane of human fibroblasts: a quantitative approach J. Cell Sci. 109 1996 687 698
    • (1996) J. Cell Sci. , vol.109 , pp. 687-698
    • Pomorski, T.1    Müller, P.2    Zimmermann, B.3    Burger, K.4    Devaux, P.F.5    Herrmann, A.6
  • 39
    • 0000934169 scopus 로고    scopus 로고
    • Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient cells does not require the Drs2 protein
    • U. Marx, T. Polawkowski, T. Pomorski, C. Lang, H. Nelson, N. Nelson, and A. Herrmann Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient cells does not require the Drs2 protein Eur. J. Biochem. 263 1999 254 263
    • (1999) Eur. J. Biochem. , vol.263 , pp. 254-263
    • Marx, U.1    Polawkowski, T.2    Pomorski, T.3    Lang, C.4    Nelson, H.5    Nelson, N.6    Herrmann, A.7
  • 40
    • 0029992825 scopus 로고    scopus 로고
    • A subfamily of P-type ATPase with aminophospholipid transporting activity
    • X. Tang, M.S. Halleck, R.A. Schlegel, and P. Williamson A subfamily of P-type ATPase with aminophospholipid transporting activity Science 272 1996 1495 1497
    • (1996) Science , vol.272 , pp. 1495-1497
    • Tang, X.1    Halleck, M.S.2    Schlegel, R.A.3    Williamson, P.4
  • 41
    • 0034121746 scopus 로고    scopus 로고
    • Is lipid translocation involved during endo- and exocytosis?
    • P.F. Devaux Is lipid translocation involved during endo- and exocytosis? Biochimie 82 2000 497 509
    • (2000) Biochimie , vol.82 , pp. 497-509
    • Devaux, P.F.1
  • 42
    • 0015245041 scopus 로고
    • Inside-outside translocation of phospholipids in vesicle membranes
    • R.D. Kornberg, and H.M. Mc Connell Inside-outside translocation of phospholipids in vesicle membranes Biochemistry 10 1971 1111 1120
    • (1971) Biochemistry , vol.10 , pp. 1111-1120
    • Kornberg, R.D.1    Mc Connell, H.M.2
  • 43
    • 0029920009 scopus 로고    scopus 로고
    • Cationic lipid binding to DNA: Characterization of complex formation
    • F.M.P. Wong, D.L. Reimer, and M.B. Bally Cationic lipid binding to DNA: characterization of complex formation Biochemistry 35 1996 5763 5776
    • (1996) Biochemistry , vol.35 , pp. 5763-5776
    • Wong, F.M.P.1    Reimer, D.L.2    Bally, M.B.3
  • 44
    • 0031872180 scopus 로고    scopus 로고
    • Characterization of lipid DNA interactions: I. Destabilization of bound lipids and DNA dissociation
    • P. Harvie, F.M.P. Wong, and M.B. Bally Characterization of lipid DNA interactions: I. Destabilization of bound lipids and DNA dissociation Biophys. J. 75 1998 1040 1051
    • (1998) Biophys. J. , vol.75 , pp. 1040-1051
    • Harvie, P.1    Wong, F.M.P.2    Bally, M.B.3
  • 45
    • 0037436945 scopus 로고    scopus 로고
    • Phosphatidylethanolamine mediated destabilization of lipid-based pDNA delivery systems
    • F.M.P. Wong, P. Harvie, Y.P. Zhang, E.C. Ramsay, and M.B. Bally Phosphatidylethanolamine mediated destabilization of lipid-based pDNA delivery systems Int. J. Pharm. 255 2003 117 127
    • (2003) Int. J. Pharm. , vol.255 , pp. 117-127
    • Wong, F.M.P.1    Harvie, P.2    Zhang, Y.P.3    Ramsay, E.C.4    Bally, M.B.5
  • 46
    • 0029067792 scopus 로고
    • The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer
    • H. Farhood, N. Serbina, and L. Huang The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer Biochim. Biophys. Acta 1235 1995 289 295
    • (1995) Biochim. Biophys. Acta , vol.1235 , pp. 289-295
    • Farhood, H.1    Serbina, N.2    Huang, L.3
  • 47
    • 0344707080 scopus 로고    scopus 로고
    • The role of helper lipids in cationic liposome-mediated gene transfer
    • S. Hui, M. Langner, Y. Zhao, P. Ross, E. Hurley, and K. Chan The role of helper lipids in cationic liposome-mediated gene transfer Biophys. J. 71 1996 590 599
    • (1996) Biophys. J. , vol.71 , pp. 590-599
    • Hui, S.1    Langner, M.2    Zhao, Y.3    Ross, P.4    Hurley, E.5    Chan, K.6
  • 48
    • 0000423625 scopus 로고    scopus 로고
    • Real time enzyme kinetics of restriction endonuclease EcoRI monitored by dual-color fluorescence cross-correlation spectroscopy
    • U. Kettling, A. Koltermann, P. Schwille, and M. Eigen Real time enzyme kinetics of restriction endonuclease EcoRI monitored by dual-color fluorescence cross-correlation spectroscopy Proc. Natl. Acad. Sci. U. S. A. 95 1998 11420 14116
    • (1998) Proc. Natl. Acad. Sci. U. S. A. , vol.95 , pp. 11420-14116
    • Kettling, U.1    Koltermann, A.2    Schwille, P.3    Eigen, M.4
  • 50
    • 0242424071 scopus 로고
    • Lipid swelling and liposome electroformation mediated by electric fields
    • D.S. Dimitrov, and M.I. Angelova Lipid swelling and liposome electroformation mediated by electric fields Bioelectrochem. Bioenerg. 19 1988 323 333
    • (1988) Bioelectrochem. Bioenerg. , vol.19 , pp. 323-333
    • Dimitrov, D.S.1    Angelova, M.I.2
  • 51
    • 0041461861 scopus 로고    scopus 로고
    • Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopy
    • N. Kahya, D. Scherfeld, K. Bacia, B. Poolman, and P. Schwille Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopy J. Biol. Chem. 278 2003 28109 28115
    • (2003) J. Biol. Chem. , vol.278 , pp. 28109-28115
    • Kahya, N.1    Scherfeld, D.2    Bacia, K.3    Poolman, B.4    Schwille, P.5
  • 52
    • 0343729418 scopus 로고    scopus 로고
    • Imaging and tracking of single GFP molecules in solution
    • U. Kubitscheck, O. Kouckmann, T. Kues, and R. Peters Imaging and tracking of single GFP molecules in solution Biophys. J. 78 2000 2170 2179
    • (2000) Biophys. J. , vol.78 , pp. 2170-2179
    • Kubitscheck, U.1    Kouckmann, O.2    Kues, T.3    Peters, R.4
  • 53
    • 0031026824 scopus 로고    scopus 로고
    • Membrane fusion with cationic liposomes: Effects of target membrane lipid composition
    • A.L. Bailey, and P.R. Cullis Membrane fusion with cationic liposomes: effects of target membrane lipid composition Biochemistry 36 1997 1628 1634
    • (1997) Biochemistry , vol.36 , pp. 1628-1634
    • Bailey, A.L.1    Cullis, P.R.2
  • 54
    • 0035807773 scopus 로고    scopus 로고
    • Mass spectrometric analysis reveals an increase in plasma membrane polyunsaturated phospholipid species upon cellular cholesterol loading
    • T.S. Blom, M. Koivusalo, E. Kuismanen, R. Kostiainen, P. Somerharju, and E. Ikonen Mass spectrometric analysis reveals an increase in plasma membrane polyunsaturated phospholipid species upon cellular cholesterol loading Biochemistry 40 2001 14635 14644
    • (2001) Biochemistry , vol.40 , pp. 14635-14644
    • Blom, T.S.1    Koivusalo, M.2    Kuismanen, E.3    Kostiainen, R.4    Somerharju, P.5    Ikonen, E.6
  • 57
    • 0033702974 scopus 로고    scopus 로고
    • Characterization of photo-induced isomerization and back-isomerization of the cyanine dye Cy-5 by fluorescence correlation spectroscopy
    • J. Widengren, and P. Schwille Characterization of photo-induced isomerization and back-isomerization of the cyanine dye Cy-5 by fluorescence correlation spectroscopy J. Chem. Phys. 104 2000 6416 6428
    • (2000) J. Chem. Phys. , vol.104 , pp. 6416-6428
    • Widengren, J.1    Schwille, P.2
  • 58
  • 63
    • 0024474697 scopus 로고
    • KMnO4 as a probe for lac promoter DNA melting and mechanism in vivo
    • S. Sasse-Dwight, and J.D. Gralla KMnO4 as a probe for lac promoter DNA melting and mechanism in vivo J. Biol. Chem. 264 1989 8074 8081
    • (1989) J. Biol. Chem. , vol.264 , pp. 8074-8081
    • Sasse-Dwight, S.1    Gralla, J.D.2


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