Applications of dual-color confocal fluorescence spectroscopy in biotechnology

Springer Series in Chemical Physics

Volumn 67, Issue , 2001, Pages 211-226

  Koltermann, Andre ^a   Kettling, U ^a   Stephan, J ^a   Rarbach, M ^a   Winkler, T ^a   Eigen, M ^a  

^a Direvo Biotech AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; COLOR; CONFERENCE PAPER; CONFORMATION; COVALENT BOND; DYNAMICS; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; ILLUMINATION; KINETICS; MATHEMATICAL ANALYSIS; MEASUREMENT;

EID: 0035775727     PISSN: 01726218     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-3-642-56544-1_12     Document Type: Article

References (19)

1. M. Auer, K. J. Moore, F. J. Meyer-Almes, R. Günther, A. J. Pope, and K. A. Stoeckli, "Fluorescence correlation spectroscopy - Lead discovery by miniaturized HTS," Drug Discovery Today 3(10), 457-465 (1998)
2. J. Bieschke and P. Schwille, "Aggregation of prion protein investigated by dual-color fluorescence cross-correlation spectroscopy," Fluorescence Microscopy and Fluorescent Probes 2, 81-86 (1998)
3. C. Eggeling, J. R. Fries, L. Brand, R. Günther, and C. A. M. Seidel, "Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy," Proc. Natl. Acad. Sci. USA 95, 1556-1561 (1998)
4. M. Ehrenberg and R. Rigler, "Rotational brownian motion and fluorescence intensity fluctuations," Chem. Phys. 4, 390-401 (1974)
5. M. Ehrenberg and R. Rigler, "Fluorescence correlation spectroscopy applied to rotational diffusion of macromolecules," Quart. Rev. Biophys. 9, 69-81 (1976)
6. M. Eigen and W. Gardiner, "Evolutionary molecular engineering based on RNA replication," Pure. Appl. Chem. 56, 967-978 (1984)
7. M. Eigen and R. Rigler, "Sorting single molecules: Application to diagnostics and evolutionary biotechnology," Proc. Natl. Acad. Sci. USA 91, 5740-5747 (1994)
8. E. L. Elson, D. Magde, and W. W. Webb, "Fluorescence correlation spectroscopy. II. An experimental realization," Biopolymers 13, 29-61 (1974)
9. U. Kettling, A. Koltermann, P. Schwille, and M. Eigen, "Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy," Proc. Natl. Acad. Sci. USA 95, 1416-1420 (1998)
10. U. Kettling, A. Koltermann, and M. Eigen, "Evolutionary biotechnology - Reflections and perspectives," Curr. Top. Microb. Immun. 243, 173-185. (1999)
11. A. Koltermann, U. Kettling, J. Bieschke, T. Winkler, and M. Eigen, "Rapid assay processing by integration of dual-color fluorescence cross-correlation spectroscopy: High throughput screening for enzyme activity," Proc. Natl. Acad. Sci. USA 95, 1421-1426 (1998)
12. A. Koltermann and U. Kettling, "Principles and methods of evolutionary biotechnology," Biophys. Chem. 66, 159-177 (1997)
13. D. Magde, E. L. Elson, and W. W. Webb, "Thermodynamic fluctuations in a reacting system - Measurement by fluorescence correlation spectroscopy," Phys. Rev. Lett. 29, 705-708 (1972)
14. R. Rigler, U. Mets, J. Widengren, and P. Kask, "Fluorescence correlation spectroscopy with high count rate and low background analysis of translational diffusion," Eur. Biophys. J. 22, 169-175 (1993)
15. P. Schwille, Fluoreszenz-Korrelations-Spektroskopie: Analyse Biochemischer Systeme auf Einzelmolekülebene, Thesis, Technische Universität Braunschweig (1996)
16. P. Schwille, U. Haupts, S. Maiti, and W. W. Webb, "Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation," Biophys. J. 77, 2251-2265 (1999)
17. P. Schwille, F. J. Meyer-Almes, and R. Rigler, "Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution," Biophys. J. 72, 1878-1886 (1997)
18. S. Sterrer and K. Henco, Fluorescence correlation spectroscopy (FCS) - A highly sensitive method to analyze drug/target interactions'," J. Recept. Signal. Transduct. Res. 17(1-3), 511-520 (1997)
19. T. Winkler, U. Kettling, A. Koltermann, and M. Eigen, "Confocal fluorescence coincidence analysis: An approach to ultra high-throughput screening," Proc. Natl. Acad. Sci. USA 96, 1375-1378 (1999)