A subfamily of P-type ATPases with aminophospholipid transporting activity

Science

Volumn 272, Issue 5267, 1996, Pages 1495-1497

  Tang, Xiaojing ^a   Halleck, Margaret S ^b   Schlegel, Robert A ^b   Williamson, Patrick ^a  

^a AMHERST COLLEGE   (United States)

^b PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; AMINOPHOSPHOLIPID; CARRIER PROTEIN; MEMBRANE ENZYME; PHOSPHATIDYLSERINE;

ARTICLE; BLOOD CLOTTING; CAENORHABDITIS ELEGANS; CATTLE; ENZYME ACTIVITY; NONHUMAN; PHAGOCYTOSIS; PHOSPHOLIPID TRANSFER; PRIORITY JOURNAL; PROTEIN FAMILY; SACCHAROMYCES CEREVISIAE;

EID: 0029992825     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5267.1495     Document Type: Article

References (33)

1. A. J. Schroit and R. F. A. Zwaal, Biochim. Biophys. Acta 1071, 313 (1991); P. Williamson and R. A. Schlegel, Mol. Membr. Biol. 11, 199 (1994).
2. A. J. Schroit and R. F. A. Zwaal, Biochim. Biophys. Acta 1071, 313 (1991); P. Williamson and R. A. Schlegel, Mol. Membr. Biol. 11, 199 (1994).
3. L. McEvoy, P. Williamson, R. A. Schlegel, Proc. Natl. Acad. Sci. U.S.A. 83, 3311 (1986); T. M. Allen, P. Williamson, R. A. Schlegel, ibid. 85, 8067 (1988); D. Pradhan, P. Williamson, R. A. Schlegel, Mol. Membr. Biol. 11, 181 (1994).
4. L. McEvoy, P. Williamson, R. A. Schlegel, Proc. Natl. Acad. Sci. U.S.A. 83, 3311 (1986); T. M. Allen, P. Williamson, R. A. Schlegel, ibid. 85, 8067 (1988); D. Pradhan, P. Williamson, R. A. Schlegel, Mol. Membr. Biol. 11, 181 (1994).
5. L. McEvoy, P. Williamson, R. A. Schlegel, Proc. Natl. Acad. Sci. U.S.A. 83, 3311 (1986); T. M. Allen, P. Williamson, R. A. Schlegel, ibid. 85, 8067 (1988); D. Pradhan, P. Williamson, R. A. Schlegel, Mol. Membr. Biol. 11, 181 (1994).
6. V. A. Fadok et al., J. Immunol. 148, 2207 (1992); V. A. Fadok et al., ibid. 149, 4029 (1992); R. A. Schlegel, M. Callahan, S. Krahling, P. Williamson, in Proceedings of the Sixth International Conference on Lymphocyte Activation and Immune Regulation: Cell Cycle and Programmed Cell Death in the Immune System, S. Gupta and J. J. Cohen, Eds. (Plenum, New York, in press).
7. V. A. Fadok et al., J. Immunol. 148, 2207 (1992); V. A. Fadok et al., ibid. 149, 4029 (1992); R. A. Schlegel, M. Callahan, S. Krahling, P. Williamson, in Proceedings of the Sixth International Conference on Lymphocyte Activation and Immune Regulation: Cell Cycle and Programmed Cell Death in the Immune System, S. Gupta and J. J. Cohen, Eds. (Plenum, New York, in press).
8. V. A. Fadok et al., J. Immunol. 148, 2207 (1992); V. A. Fadok et al., ibid. 149, 4029 (1992); R. A. Schlegel, M. Callahan, S. Krahling, P. Williamson, in Proceedings of the Sixth International Conference on Lymphocyte Activation and Immune Regulation: Cell Cycle and Programmed Cell Death in the Immune System, S. Gupta and J. J. Cohen, Eds. (Plenum, New York, in press).
9. M. Seigneuret and P. F. Devaux, Proc. Natl. Acad. Sci. U.S.A. 81, 3751 (1984); A. Zachowski, A. Herrmann, A. Paraf, P. F. Devaux, Biochim. Biophys. Acta 897, 197 (1987); O. C. Martin and R. E. Pagano, J. Biol. Chem. 262, 5890 (1987); A. Bogdanov, B. Verhoven, R. A. Schlegel, P. Williamson, Biochem. Soc. Trans. 21, 271 (1993).
10. M. Seigneuret and P. F. Devaux, Proc. Natl. Acad. Sci. U.S.A. 81, 3751 (1984); A. Zachowski, A. Herrmann, A. Paraf, P. F. Devaux, Biochim. Biophys. Acta 897, 197 (1987); O. C. Martin and R. E. Pagano, J. Biol. Chem. 262, 5890 (1987); A. Bogdanov, B. Verhoven, R. A. Schlegel, P. Williamson, Biochem. Soc. Trans. 21, 271 (1993).
11. M. Seigneuret and P. F. Devaux, Proc. Natl. Acad. Sci. U.S.A. 81, 3751 (1984); A. Zachowski, A. Herrmann, A. Paraf, P. F. Devaux, Biochim. Biophys. Acta 897, 197 (1987); O. C. Martin and R. E. Pagano, J. Biol. Chem. 262, 5890 (1987); A. Bogdanov, B. Verhoven, R. A. Schlegel, P. Williamson, Biochem. Soc. Trans. 21, 271 (1993).
12. M. Seigneuret and P. F. Devaux, Proc. Natl. Acad. Sci. U.S.A. 81, 3751 (1984); A. Zachowski, A. Herrmann, A. Paraf, P. F. Devaux, Biochim. Biophys. Acta 897, 197 (1987); O. C. Martin and R. E. Pagano, J. Biol. Chem. 262, 5890 (1987); A. Bogdanov, B. Verhoven, R. A. Schlegel, P. Williamson, Biochem. Soc. Trans. 21, 271 (1993).
13. A. Zachowski, J. P. Henry, P. F. Devaux, Nature 340, 75 (1989).
14. A. Zachowski and Y. Morot Gaudry-Talarmain, J. Neurochem. 55, 1352 (1990).
15. G. Morrot, A. Zachowski, P. F. Devaux, FEBS Lett. 266, 29 (1990); M. E. Auland, B. D. Roufogalis, P. F. Devaux, A. Zachowski, Proc. Natl. Acad. Sci. U.S.A. 91, 10938 (1994).
16. G. Morrot, A. Zachowski, P. F. Devaux, FEBS Lett. 266, 29 (1990); M. E. Auland, B. D. Roufogalis, P. F. Devaux, A. Zachowski, Proc. Natl. Acad. Sci. U.S.A. 91, 10938 (1994).
17. Y. Moriyama and N. Nelson, J. Biol. Chem. 263, 8521 (1988).
18. B. W. Hicks and S. M. Parsons, J. Neurochem. 58, 1211 (1992); X. Xie, D. K. Stone, E. Racker, J. Biol. Chem. 264, 1710 (1989).
19. B. W. Hicks and S. M. Parsons, J. Neurochem. 58, 1211 (1992); X. Xie, D. K. Stone, E. Racker, J. Biol. Chem. 264, 1710 (1989).
20. T. L. Ripmaster, G. P. Vaughn, J. L. Woolford, Mol. Cell. Biol. 13, 7901 (1993).
21. S. Krishna, G. M. Cowan, K. J. Robson, J. C. Meade, Exp. Parasitol. 78, 113 (1994); F. Trottein and A. Cowman, Eur. J. Biochem. 227, 214 (1995).
22. S. Krishna, G. M. Cowan, K. J. Robson, J. C. Meade, Exp. Parasitol. 78, 113 (1994); F. Trottein and A. Cowman, Eur. J. Biochem. 227, 214 (1995).
23. R. Wilson et al., Nature 368, 32 (1994).
24. H. Li, X. Cui, N. Amheim, Proc. Natl. Acad. Sci. U.S.A. 87, 4580 (1990).
25. D. Eisenberg, E. Swarz, M. Kamaromy, R. Wall, J. Mol. Biol. 179, 125 (1984).
26. M. J. Fagan and M. H. Saier Jr., J. Mol. Evol. 38, 57 (1994).
27. L. S. Kean, R. S. Fuller, J. W. Nichols, J. Cell Biol. 123, 1403 (1993).
28. D. M. Clarke, T. W. Loo, G. Inesi, D. H. MacLennan, Nature 339, 476 (1989).
29. B. Verhoven, R. A. Schlegel, P. Williamson, J. Exp. Med. 182, 1597 (1995).
30. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M. Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
31. S. B. Needleman and C. D. Wunsch, J. Mol. Biol. 48, 443 (1970).
32. 6-NBD-sn-glycero-3-phosphoserine or -phosphocholine; Avanti Polar Lipids) was added to a final concentration of 1 mM from a stock solution in dimethylsulfoxide. After 1 min on ice, the cells were centrifuged; the supernatant was discarded and the cells were resuspended in PBSSA. Immediately upon resuspension and at 1, 2, 5, and 9 min thereafter, four portions (50 μl) were withdrawn, two of which were each mixed with 5 μl of fatty acid-free BSA (Sigma, 10% w/v) in PBSSA, and two of which were mixed with 5 μl of PBSSA alone. After 1 min on ice, the cells were sedimented and 45 μl of the supernatant was transferred to 1500 μl of 1% Triton X-100 in water. Fluorescence intensity was measured at 530 nm during excitation at 470 nm.
33. We thank R. Goldsby for the calf thymus cDNA library, J. Woolford for yeast strains, D. Pradhan for sharing mouse ATPase sequence information, and the Microchemistry Facility at Harvard University for amino acid sequencing. Supported by Amherst College Faculty Research Award Program grant 3-134-794-029.