Validation of IKKβ as therapeutic target in airway inflammatory disease by adenoviral-mediated delivery of dominant-negative IKKβ to pulmonary epithelial cells

British Journal of Pharmacology

Volumn 145, Issue 1, 2005, Pages 114-122

  Catley, Matthew C ^a   Chivers, Joanna E ^a   Holden, Neil S ^b   Barnes, Peter J ^a   Newton, Robert ^c  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

^c UNIVERSITY OF CALGARY   (Canada)

Author keywords

Apoptosis; Cytokines; GM CSF; I kappa B kinase; IL 8; Inflammation; NF kappa B; Prostaglandin GH synthase; Pulmonary epithelium; Signal transduction

Indexed keywords

CASPASE 3; CYCLOOXYGENASE 2; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; I KAPPA B ALPHA; I KAPPA B BETA; I KAPPA B KINASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERCELLULAR ADHESION MOLECULE 1; INTERLEUKIN 1BETA; INTERLEUKIN 8; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROSTAGLANDIN SYNTHASE; SYNAPTOTAGMIN; TUMOR NECROSIS FACTOR ALPHA;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIINFLAMMATORY ACTIVITY; APOPTOSIS; ARTICLE; ASTHMA; BRONCHUS HYPERREACTIVITY; CELL VIABILITY; CONTROLLED STUDY; DNA BINDING; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; GENE OVEREXPRESSION; GENE TARGETING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; KNOCKOUT MOUSE; LUNG ALVEOLUS EPITHELIUM; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; VIRUS GENE;

ADENOVIRIDAE; APOPTOSIS; ASTHMA; CELL LINE; EPITHELIAL CELLS; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; I-KAPPA B KINASE; NF-KAPPA B; PROTEIN-SERINE-THREONINE KINASES; RESPIRATORY MUCOSA; TRANSCRIPTION, GENETIC;

EID: 18744412122     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0706170     Document Type: Article

References (29)

1. ANEST, V., HANSON, J.L., COGSWELL, P.C., STEINBRECHER, K.A., STRAHL, B.D. & BALDWIN, A.S. (2003). A nucleosomal function for IkappaB kinase-alpha in NF-kappaB-dependent gene expression. Nature, 423, 659-663.
2. BARNES, P.J. (1999). Therapeutic strategies for allergic diseases. Nature, 402 (suppl), B31-B38.
3. BERGMANN, M., BARNES, P.J. & NEWTON, R. (2000). Molecular regulation of granulocyte-macrophage colony-stimulating factor in human lung epithelial cells by IL-1, IL-4 and IL-13 involves both transcriptional and post-transcriptional mechanisms. Am. J. Respir. Cell Mol. Biol., 22, 582-589.
4. BOUSQUET, J., JEFFERY, P.K., BUSSE, W.W., JOHNSON, M. & VIGNOLA, A.M. (2000). Asthma. From bronchoconstriction to airways inflammation and remodeling. Am. J. Respir. Crit. Care Med., 161, 1720-1745.
5. CATLEY, M.C., CHIVERS, J.E., CHIVERS, J.E., CAMBRIDGE, L.M., HOLDEN, N., SLATER, D.M., STAPLES, K.J., BERGMANN, M.W., LOSER, P., BARNES, P.J. & NEWTON, R. (2003). IL-1beta-dependent activation of NF-kappaB mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. FEBS Lett., 547, 75-79.
6. CONRON, M., ANDREAKOS, E., PANTELIDIS, P., SMITH, C, BEYNON, H.L., DUBOIS, R.M. & FOXWELL, B.M. (2002). Nuclear factor-kappaB activation in alveolar macrophages requires IkappaB kinase-beta, but not nuclear factor-kappaB inducing kinase. Am. J. Respir. Crit. Care Med., 165, 996-1004.
7. FERNANDES-ALNEMRI, T., ARMSTRONG, R.C., KREBS, J., SRINIVASULA, S.M., WANG, L., BULLRICH, F., FRITZ, L.C., TRAPANI, J.A., TOMASELLI, K.J., LITWACK, G. & ALNEMRI, E.S. (1996). In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. U.S.A., 93, 7464-7469.
8. FERNANDES-ALNEMRI, T., LITWACK, G. & ALNEMRI, E.S. (1994). CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem., 269, 30761-30764.
9. GHOSH, S. & KARIN, M. (2002). Missing pieces in the NF-kappaB puzzle. Cell, 109 (Suppl), S81-S96.
10. IMLER, J.L. & HOFFMANN, J.A. (2001). Toll receptors in innate immunity. Trends Cell Biol., 11, 304-311.
11. KARIN, M. & LIN, A. (2002). NF-kappaB at the crossroads of life and death. Nat. Immunol., 3, 221-227.
12. KRAPPMANN, D., WULCZYN, F.G. & SCHEIDEREIT, C. (1996). Different mechanisms control signal-induced degradation and basal turnover of the NF-kappaB inhibitor IkappaB alpha in vivo. EMBO J., 15, 6716-6726.
13. LI, Q., VAN ANTWERP, D., MERCURIO, F., LEE, K.F. & VERMA, I.M. (1999a). Severe liver degeneration in mice lacking the IkappaB kinase 2 gene. Science, 284, 321-325.
14. LI, X., MASSA, P.E., HANIDU, A., PEET, G.W., ARO, P., SAVITT, A., MISCHE, S., LI, J. & MARCU, K.B. (2002). IKKalpha, IKKbeta, and NEMO/IKKgamma are each required for the NF-kappa B-mediated inflammatory response program. J. Biol. Chem., 277, 45129-45140.
15. LI, Z.W., CHU, W., HU, Y., DELHASE, M., DEERINCK, T., ELLISMAN, M., JOHNSON, R. & KARIN, M. (1999b). The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis. J. Exp. Med., 189, 1839-1845.
16. MATSUSHIMA, A., KAISHO, T., RENNERT, P.D., NAKANO, H., KUROSAWA, K., UCHIDA, D., TAKEDA, K., AKIRA, S. & MATSUMOTO, M. (2001). Essential role of nuclear factor (NF)-kappaB-inducing kinase and inhibitor of kappaB (IkappaB) kinase alpha in NF-kappaB activation through lymphotoxin beta receptor, but not through tumor necrosis factor receptor I. J. Exp. Med., 193, 631-636.
17. MCKAY, R.A., MIRAGLIA, L.J., CUMMINS, L.L., OWENS, S.R., SASMOR, H. & DEAN, N.M. (1999). Characterization of a potent and specific class of antisense oligonucleotide inhibitor of human protein kinase C-alpha expression. J. Biol. Chem., 274, 1715-1722.
18. MERCURIO, F., ZHU, H., MURRAY, B.W., SHEVCHENKO, A., BENNETT, B.L., LI, J., YOUNG, D.B., BARBOSA, M., MANN, M., MANNING, A. & RAO, A. (1997). IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science, 278, 860-866.
19. MITCHELL, J.A., BELVISI, M.G., AKARASEREENONT, P., ROBBINS, R.A., KWON, O.J., CROXTALL, J., BARNES, P.J. & VANE, J.R. (1994). Induction of cyclo-oxygenase-2 by cytokines in human pulmonary epithelial cells: regulation by dexamethasone. Br. J. Pharmacol., 113, 1008-1014.
20. NASUHARA, Y., ADCOCK, I.M., CATLEY, M., BARNES, P.J. & NEWTON, R. (1999). Differential IKK activation and IkappaBalpha degradation by interleukin-1beta and tumor necrosis factor-alpha in human U937 monocytic cells: evidence for additional regulatory steps in kappaB-dependent transcription. J. Biol. Chem., 274, 19965-19972.
21. NEWTON, R., ADCOCK, I.M. & BARNES, P.J. (1996). Superinduction of NF-kappa B by actinomycin D and cycloheximide in epithelial cells. Biochem. Biophys. Res. Commun., 218, 518-523.
22. OLIVER, F.J., DE LA, R.G., ROLLI, V., RUIZ-RUIZ, M.C., DE MURCIA, G. & MURCIA, J.M. (1998). Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant. J. Biol. Chem., 273, 33533-33539.
23. ORLOWSKI, R.Z. & BALDWIN Jr, A.S. (2002). NF-kappaB as a therapeutic target in cancer. Trends Mol. Med., 8, 385-389.
24. PASPARAKIS, M., COURTOIS, G., HAFNER, M., SCHMIDT-SUPPRIAN, M., NENCI, A., TOKSOY, A., KRAMPERT, M., GOEBELER, M., GILLITZER, R., ISRAEL, A., KRIEG, T., RAJEWSKY, K. & HAASE, I. (2002). TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2. Nature, 417, 861-866.
25. SENFTLEBEN, U., LI, Z.W., BAUD, V. & KARIN, M. (2001). IKKbeta is essential for protecting T cells from TNFalpha-induced apoptosis. Immunity, 14, 217-230.
26. SOLDANI, C. & SCOVASSI, A.I. (2002). Poly(ADP-ribose) polymerase-1 cleavage during apoptosis: an update. Apoptosis, 7, 321-328.
27. TANG, G., YANG, J., MINEMOTO, Y. & LIN, A. (2001). Blocking caspase-3-mediated proteolysis of IKKbeta suppresses TNF-alpha-induced apoptosis. Mol. Cell, 8, 1005-1016.
28. YAMAMOTO, Y., VERMA, U.N., PRAJAPATI, S., KWAK, Y.T. & GAYNOR, R.B. (2003). Histone H3 phosphorylation by IKK-alpha is critical for cytokine-induced gene expression. Nature, 423, 655-659.
29. ZANDI, E., ROTHWARF, D.M., DELHASE, M., HAYAKAWA, M. & KARIN, M. (1997). The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell, 91, 243-252.