Improved efficiency of inverse PCR mutagenesis

BioTechniques

Volumn 21, Issue 4, 1996, Pages 604-608

  Dorrell, N ^a   Gyselman, V G ^a   Foynes, S ^a   Li, S R ^a   Wren, B W ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; PLASMID DNA;

ARTICLE; DNA DETERMINATION; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029766881     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214bm07     Document Type: Article

References (4)

1. Du, Z., D.A. Regier and R.C. Desrosiers. 1995. Improved recombination PCR mutagenesis procedure that uses alkaline-denatured plasmid template. BioTechniques 18:376-378.
2. Fleischmann, R.D., M.D. Adams, O. White, R.A. Clayton, E.F. Kirkness, A.R. Kerlavage, C.J. Bult, J.-F. Tomb et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
3. Gatlin, J., L.H. Campbell, M.G. Schmidt and S.J. Arrigo. 1995. Direct-rapid (DR) mutagenesis of large plasmids using PCR. BioTechniques 19:559-564.
4. Wren, B.W., J. Henderson and J.M. Ketley. 1994. A PCR-based strategy for the rapid construction of defined bacterial deletion mutants. BioTechniques 16:994-996.