Autolytic activation and localization in Schneider cells (S2) of calpain B from Drosophila

Biochemical Journal

Volumn 378, Issue 2, 2004, Pages 299-305

  Farkas, Attila ^a   Tompa, Peter ^a   Schád, Éva ^a   Sinka, Rita ^a   Jékely, Gáspár ^a   Friedrich, Peter ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

Autolysis; Calpain; Drosophila; Expression pattern; Translocation

Indexed keywords

CELLS; ENZYMES; IMMUNOLOGY; MUTAGENESIS; RNA;

AUTOLYSIS; HYBRIDIZATION;

BIOCHEMISTRY;

CALPAIN; MESSENGER RNA;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOLYSIS; CELL ACTIVATION; DEVELOPMENTAL STAGE; DROSOPHILA MELANOGASTER; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LARVA; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SALIVARY GLAND; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; CALPAIN; CELL LINE; CYTOPLASM; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ENZYME ACTIVATION; INTRACELLULAR MEMBRANES; MOLECULAR SEQUENCE DATA; RNA, MESSENGER;

ARACHNIDA; DROSOPHILA MELANOGASTER; HEXAPODA; MELANOGASTER;

EID: 1642406178     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031310     Document Type: Article

References (32)

1. Ono, Y., Sorimachi, H. and Suzuki, K. (1998) Structure and physiology of calpain, an enigmatic protease. Biochem. Biophys. Res. Commun. 245, 289-294
2. Sorimachi, H. and Suzuki, K. (2001 ) The structure of calpain. J. Biochem. (Tokyo) 129, 653-664
3. Margis, R. and Margis-Pinheiro, M. (2003) Phytocalpains: orthologous calcium-dependent cysteine proteases. Trends Plant Sci. 8, 58-62
4. Hosfield, C. M., Elce, J. S., Davies, P. L. and Jia, Z. (1999) Crystal structure of calpain reveals the structural basis for Ca(2+)-dependent protease activity and a novel mode of enzyme activation. EMBO J. 18, 6880-6889
5. Strobl, S., Fernandez-Catalan, C., Braun, M., Huber, R., Masumoto, H., Nakagawa, K., Irie, A., Sorimachi, H., Bourenkow, G., Bartunik, H., Suzuki, K. and Bode, W. (2000) The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc. Natl. Acad. Sci. U.S.A. 97, 588-592
6. Yoshizawa, T., Sorimachi, H., Tomioka, S., Ishiura, S. and Suzuki, K. (1995) A catalytic subunit of calpain possesses full proteolytic activity. FEBS Lett. 358, 101-103
7. Schád, E., Farkas, A., Jékely, G., Tompa, P. and Friedrich, P. (2002) A novel human small subunit of calpains. Biochem. J. 362, 383-388
8. Todd, B., Moore, D., Deivanayagam, C. C., Lin, G. D., Chattopadhyay, D., Maki, M., Wang, K. K. and Narayana, S. V. (2003) A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor. J. Mol. Biol. 328, 131-146
9. Horikawa, Y., Oda, N., Cox, N. J., Li, X., Orho-Melander, M., Hara, M., Hinokio, Y., Lindner, T. H., Mashima, H., Schwarz, P. E. et al. (2000) Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus. Nat. Genet. 26, 163-175
10. Richard, I., Broux, O., Allamand, V., Fougerousse, F., Chianniikulchai, N., Bourg, N., Brenguier, L., Devaud, C., Pasturaud, P. and Roudaut, C. (1995) Mutations in the proteolytic enzyme calpain 3 cause limb-girdle muscular dystrophy type 2A. Cell 81, 27-40
11. Pintér, M. and Friedrich, P. (1988) The calcium-dependent proteolytic system calpain-calpastatin in Drosophila melanogaster. Biochem. J. 253, 467-473
12. Pintér, M., Stierandova, A. and Friedrich, P. (1992) Purification and characterization of a Ca(2+)-activated thiol protease from Drosophila melanogaster. Biochemistry 31, 8201-8206
13. Theopold, U., Pintér, M., Daffre, S., Tryselius, Y., Friedrich, P., Nassel, D. R. and Hultmark, D. (1995) CalpA, a Drosophila calpain homolog specifically expressed in a small set of nerve, midgut, and blood cells. Mol. Cell. Biol. 15, 824-834
14. Jékely, G. and Friedrich, P. (1999) Characterization of two recombinant Drosophila calpains. CALPA and a novel homolog, CALPB. J. Biol. Chem. 274, 23893-23900
15. Spadoni, C., Farkas, A., Sinka, R., Tompa, P. and Friedrich, P. (2003) Molecular cloning and RNA expression of a novel Drosophila calpain, Calpain C. Biochem. Biophys. Res. Commun. 303, 343-349
16. Delaney, S. J., Hayward, D. C., Barleben, F., Fischbach, K. F. and Miklos, G. L. (1991) Molecular cloning and analysis of small optic lobes, a structural brain gene of Drosophila melanogaster. Proc. Natl. Acad. Sci. U.S.A. 88, 7214-7218
17. Brons-Poulsen, J., Petersen, N. E., Horder, M. and Kristiansen, K. (1998) An improved PCR-based method for site directed mutagenesis using megaprimers. Mol. Cell. Probes 12, 345-348
18. Ephrussi, A., Dickinson, L. K. and Lehmann, R. (1991) Oskar organizes the germ plasm and directs localization of the posterior determinant nanos. Cell 66, 37-50
19. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685
20. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354
21. Tompa, P. (2002) Intrinsically unstructured proteins. Trends Biochem. Sci. 27, 527-533
22. Reiersen, H. and Rees, A. R. (2001) The hunchback and its neighbours: proline as an environmental modulator. Trends Biochem. Sci. 26, 679-684
23. Saido, T. C., Nagao, S., Shiramine, M., Tsukaguchi, M., Sorimachi, H., Murofushi, H., Tsuchiya, T., Ito, H. and Suzuki, K. (1992) Autolytic transition of mu-calpain upon activation as resolved by antibodies distinguishing between the pre- and post-autolysis forms. J. Biochem. (Tokyo) 111, 81-86
24. 2+ ions. J. Biochem. (Tokyo) 90, 275-278
25. DeMartino, G. N., Huff, C. A. and Croall, D. E. (1986) Autoproteolysis of the small subunit of calcium-dependent protease II activates and regulates protease activity. J. Biol. Chem. 261, 12047-12052
26. 2+ requirement, and heterodimer stability in m-calpain. J. Biol. Chem. 272, 11268-11275
27. Baki, A., Tompa, P., Alexa, A., Molnár, O. and Friedrich, P. (1996) Autolysis parallels activation of mu-calpain. Biochem. J. 318, 897-901
28. Glading, A., Lauffenburger, D. A. and Wells, A. (2002) Cutting to the chase: calpain proteases in cell motility. Trends Cell. Biol. 12, 46-54
29. Matus, A. (2001) GFP moves on. Trends Cell. Biol. 11, 183
30. Jockusch, H., Voigt, S. and Eberhard, D. (2003) Localization of GFP in frozen sections from unfixed mouse tissues: immobilization of a highly soluble marker protein by formaldehyde vapor. J. Histochem. Cytochem. 51, 401-404
31. Denning, D. P., Patel, S. S., Uversky, V., Fink, A. L. and Rexach, M. (2003) Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded. Proc. Natl. Acad. Sci. U.S.A. 100, 2450-2455
32. Mellgren, R. L. and Lu, Q. (1994) Selective nuclear transport of mu-calpain. Biochem. Biophys. Res. Commun. 204, 544-550