An improved PCR-based method for site directed mutagenesis using megaprimers

Molecular and Cellular Probes

Volumn 12, Issue 6, 1998, Pages 345-348

  Brons Poulsen J ^a,b   Petersen, N E ^b   Horder M ^b   Kristiansen, K ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b ODENSE UNIVERSITY HOSPITAL   (Denmark)

Author keywords

PCR; Site directed mutagenesis; Structure function relationship

Indexed keywords

DNA POLYMERASE;

ARTICLE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 0032420269     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1998.0187     Document Type: Article

References (5)

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3. Jones D. H., Winistorfer S. C. Recombinant circle PCR and recombination PCR for site-specific mutagenesis without PCR product purification. BioTechniques. 12:1992;528-535.
4. Sambrook J., Fritsch E., Maniatis T. Molecular Cloning. A laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press. 1989.
5. Lundberg, K. S. Shoemaker, D. D. Adams, M. W. W. Short, J. M. Sorge, J. A. Mathur, E. J. 1991, High fidelity amplification using a thermostable DNA polymerase isolated from.