Correlation between Catalytic Efficiency and the Transcription Read-Out in Chemical Complementation: A General Assay for Enzyme Catalysis

Biochemistry

Volumn 43, Issue 12, 2004, Pages 3570-3581

  Sengupta, Debleena ^a   Lin, Hening ^a   Goldberg, Shalom D ^a   Mahal, Jacqueline J ^a   Cornish, Virginia W ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CATALYSIS; CORRELATION METHODS; PURIFICATION;

CATALYTIC EFFICIENCY; TRANSCRIPTION READ-OUT;

ENZYMES;

BETA GALACTOSIDASE; BETA LACTAMASE; DEXAMETHASONE CEPHEM METHOTREXATE DIMER; DIMER; ENZYME VARIANT; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CHEMICAL COMPLEMENTATION; CHEMICAL REACTION; CORRELATION ANALYSIS; DIRECTED MOLECULAR EVOLUTION; ENZYME ASSAY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE MUTATION; GENETIC COMPLEMENTATION; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; HYBRIDIZATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEOMICS; REPORTER GENE; TRANSCRIPTION REGULATION; YEAST;

BETA-LACTAMASES; CATALYSIS; CEPHALOSPORINS; DEXAMETHASONE; DIRECTED MOLECULAR EVOLUTION; ENTEROBACTER CLOACAE; GENES, REPORTER; HYDROLYSIS; KINETICS; LAC OPERON; METHOTREXATE; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC; TWO-HYBRID SYSTEM TECHNIQUES; VARIATION (GENETICS);

FELIS CATUS;

EID: 1642275075     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035810c     Document Type: Article

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