Stabilization of α-chymotrypsin by chemical modification with monoamine cyclodextrin

Process Biochemistry

Volumn 40, Issue 6, 2005, Pages 2091-2094

  Fernández, Michael ^a   Fragoso, Alex ^b   Cao, Roberto ^b   Villalonga, Reynaldo ^a  

^a UNIVERSITY OF MATANZAS   (Cuba)

^b UNIVERSITY OF HAVANA   (Cuba)

Author keywords

Chymotrypsin; Aminated cyclodextrin; Enzyme stability; Modified enzyme

Indexed keywords

CHEMICAL MODIFICATION; ESTERS; POLYSACCHARIDES; STABILIZERS (AGENTS); THERMAL EFFECTS;

CHYMOTRYPSIN; CYCLODEXTRIN (CD); ESTERASIC ACTIVITY; OLIGOSACCHARIDE; PROTEOLYTIC ACTIVITY;

ENZYMES;

BOVINAE;

EID: 13844321001     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2004.07.023     Document Type: Article

References (28)

1. R. Villalonga, L. Gómez, H.L. Ramírez, and M.L. Villalonga Stabilization of α-amylase by chemical modification with carboxymethylcellulose J Chem Technol Biotechnol 74 1999 635 638
2. R.A.K. Srivastava Studies on stabilization of amylase by covalent coupling to soluble polysaccharides Enzyme Microb Technol 13 1991 164 170
3. P.V. Sundaram, and R. Venkatesh Retardation of thermal and urea induced inactivation of α-chymotrypsin by modification with carbohydrate polymers Protein Eng 11 1998 691 698
4. L. Gómez, and R. Villalonga Functional stabilization of invertase by covalent modification with pectin Biotechnol Lett 22 2000 1191 1195
5. R. Darias, and R. Villalonga Functional stabilization of cellulase by covalent modification with chitosan J Chem Technol Biotechnol 76 2001 489 493
6. L. Gómez, H.L. Ramírez, and R. Villalonga Chemical modification of α-amylase by sodium alginate Acta Biotechnol 21 2001 265 273
7. C. Wang, M. Eufemi, C. Turano, and A. Giartosio Influence of the carbohydrate moiety on the stability of glycoproteins Biochemistry 35 1996 7299 7307
8. J. Masárová, D. Mislovičová, P. Gemeiner, and E. Michalková Stability enhancement of Escherichia coli penicillin G acylase by glycosylation with yeast mannan Biotechnol Appl Biochem 34 2001 127 133
9. L. Gómez, H.L. Ramírez, and R. Villalonga Stabilization of invertase by modification of sugar chains with chitosan Biotechnol Lett 22 2000 347 350
10. H.L. Ramírez, B. Chico, K. Hoste, E.H. Schacht, and R. Villalonga Invertase stabilization by chemical modification of sugar chains with carboxymethylcellulose J Bioactive Compat Polym 17 2002 161 172
11. R. Villalonga, M.L. Villalonga, and L. Gómez Preparation and functional properties of trypsin modified by carboxymethylcellulose J Mol Catal B Enzymatic 10 2000 483 490
12. N. Rajalakshmi, and P.V. Sundaram Stability of native and covalently modified papain Protein Eng 8 1995 1039 1047
13. M. Fernández, A. Fragoso, R. Cao, M. Baños, and R. Villalonga Chemical conjugation of trypsin with monoamine derivatives of cyclodextrins. Catalytic and stability properties Enzyme Microb Technol 31 2002 543 548
14. R. Villalonga, M. Fernández, A. Fragoso, R. Cao, P. Di Pierro, and L. Mariniello Transglutaminase-catalyzed synthesis of trypsin-cyclodextrin conjugates. Kinetics and stability properties Biotechnol Bioeng 81 2003 732 737
15. M. Fernández, A. Fragoso, R. Cao, and R. Villalonga Improved functional properties of trypsin modified by monosubstituted amino-β-cyclodextrins J Mol Catal B Enzymatic 21 2003 133 141
16. J. Szejtli Introduction and general overview of cyclodextrins Chem Rev 98 1998 1743 1754
17. M. Fernández, M.L. Villalonga, J. Caballero, A. Fragoso, R. Cao, and R. Villalonga Effects of β-cyclodextrin-dextran polymer on stability properties of trypsin Biotechnol Bioeng 83 2003 743 747
18. M. Laskowski Trypsinogen and trypsin Methods Enzymol 2 1955 26 36
19. G.B. Schwert, and Y. Takenaka A spectrophotometric determination of trypsin and chymotrypsin Biochim Biophys Acta 16 1955 570 575
20. A. Fragoso, R. Cao, and R. Villalonga Superoxide dismutase mimetic activity of the metal (II) complexes of a dithiocarbamate derivative of β-cyclodextrin J Carbohydr Chem 14 1995 1389 1396
21. N. Zhong, H.S. Byun, and R. Bittman A convenient method for the monotosylation of β-cyclodextrin on the primary side Tetrahedron Lett 38 1998 2919 2920
22. M.K. Dubois, A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith Colorimetric method for determination of sugars and related substances Anal Chem 28 1956 350 356
23. O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R. Randall Protein measurement with the Folin phenol reagent J Biol Chem 193 1951 265 275
24. M. Fernández, M.L. Villalonga, A. Fragoso, R. Cao, and R. Villalonga Stabilization of α-chymotrypsin by modification with β-cyclodextrin derivatives Biotechnol Appl Biochem 36 2002 235 239
25. B. Wroblowski, J.F. Diaz, J. Schlitter, and Y. Engelborghs Modelling patways of α-chymotrypsin activation and deactivation Protein Eng 10 1997 1163 1174
26. V.V. Mozhaev, V.A. Šikšnis, N.S. Melik-Nubarov, N.Z. Galkantaite, G.J. Denis, and E.P. Butkus Protein stabilization via hydrophilization. Covalent modification of trypsin and α-chymotrypsin Eur J Biochem 173 1988 147 154
27. M. Fernández, A. Fragoso, R. Cao, M. Baños, M.L. Villalonga, and R. Villalonga Stabilization of trypsin by chemical modification with β-cyclodextrin monoaldehyde Biotechnol Lett 24 2002 1455 1459
28. R. Darias, I. Herrera, A. Fragoso, R. Cao, and R. Villalonga Supramolecular interactions mediated thermal stabilization for α-amylase modified with a β-cyclodextrin-carboxymethylcellulose polymer Biotechnol Lett 24 2002 1665 1668