Stabilization of trypsin by chemical modification with β-cyclodextrin monoaldehyde

Biotechnology Letters

Volumn 24, Issue 17, 2002, Pages 1455-1459

  Fernández, Michael ^a   Fragoso, Alex ^b   Cao, Roberto ^b   Baños, Maysa ^b   Villalonga, Maria L ^a   Villalonga, Reynaldo ^a  

^a UNIVERSITY OF MATANZAS   (Cuba)

^b UNIVERSITY OF HAVANA   (Cuba)

Author keywords

Cyclodextrin; Enzyme stability; Modified enzyme; Trypsin

Indexed keywords

BETA CYCLODEXTRIN ALDEHYDE; BETA CYCLODEXTRIN DERIVATIVE; BORATE SODIUM; TRYPSIN; UNCLASSIFIED DRUG;

ALKYLATION; ARTICLE; CONJUGATION; ENERGY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME STABILITY; INCUBATION TIME; PH; REDUCTION; TEMPERATURE; THERMOSTABILITY;

EID: 0036735690     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1019835522979     Document Type: Article

References (20)

1. Chemical modification of pullulan: I. Periodate oxidation
2. Cyclodextrinadamantanecarboxylate inclusion complexes: Studies of the variation in cavity size
3. Functional stabilization of cellulase by covalent modification with chitosan
4. Functional stabilization of invertase by covalent modification with pectin
5. Stabilization of invertase by modification of sugar chains with chitosan
6. Trypsinogen and trypsin
7. A novel chemoenzymatic glycosylation strategy: Application to lysozyme modification
8. Thermostability of modified enzymes: A detailed study
9. Enzyme stabilization by covalent attachment of carbohydrate
10. Modification of α-amylase from Bacillus licheniformis by the polyaldehyde derived from β-cyclodextrine and α-amylase thermostability
11. Protein stabilization via hydrophilization. Covalent modification of trypsin and α-chymotrypsin
12. Conformational stability of globular proteins
13. Perspectives for chemical modifications of enzymes
14. A spectrophotometric determination of trypsin and chymotrypsin
15. Studies on stabilization of amylase by covalent coupling to soluble polysaccharidcs
16. Retardation of thermal and urea induced inactivation of α-chymotrypsin by moditication with carbohydrate polymers
17. Introduction and general overwiew of cyclodextrins
18. Increased thermal stability of glucose dehydrogenase by cross-linking chemical modification
19. A general method for the synthesis of cyclodextrinyl aldehydes and carboxylic acids
20. A convenient method for the monotosylation of β-cyclodextrin on the primary side