Effects of β-cyclodextrin-dextran polymer on stability properties of trypsin

Biotechnology and Bioengineering

Volumn 83, Issue 6, 2003, Pages 743-747

  Fernández, Michael ^a   Villalonga, Maria L ^a   Caballero, Julio ^a   Fragoso, Alex ^b   Cao, Roberto ^b   Villalonga, Reynaldo ^a  

^a UNIVERSITY OF MATANZAS   (Cuba)

^b UNIVERSITY OF HAVANA   (Cuba)

Author keywords

cyclodextrin; Dextran; Enzyme stability; Trypsin

Indexed keywords

ACTIVATION ENERGY; ALKALINITY; FREE ENERGY; PH EFFECTS; POLYSACCHARIDES; THERMODYNAMIC STABILITY;

THERMOINACTIVATION;

ENZYMES;

6 PENTYLENEDIAMINO 6 DEOXY BETA CYCLODEXTRIN; BETA CYCLODEXTRIN DERIVATIVE; DEXTRAN; POLYMER; TRYPSIN; UNCLASSIFIED DRUG;

ALKALINITY; ARTICLE; CHEMICAL ANALYSIS; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STABILITY; NONHUMAN; TEMPERATURE; THERMOSTABILITY;

ANIMALS; CATTLE; CELLULOSE; CYCLODEXTRINS; DEXTRANS; ENZYME ACTIVATION; ENZYME STABILITY; KINETICS; PANCREAS; TEMPERATURE; TRYPSIN;

EID: 0042522510     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10707     Document Type: Article

References (24)

1. Back JB, Oakenfull D, Smith MB, 1979. Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry 18:5191-5196.
2. Cooper A. 1992. Effect of cyclodextrins on the thermal stability of globular proteins. J Am Chem Soc 114:9208-9209.
3. Cromwell WC, Byström K, Eftink MR. 1985. Cyclodextrin-adamantane-carboxylate inclusion complexes: studies of the variation in cavity size. J Phys Chem 89:326-333.
4. Darias R, Villalonga R. 2001. Functional stabilization of cellulase by covalent modification with chitosan. J Chem Technol Biotechnol 76:489-493.
5. Fernández M, Fragoso A, Cao R, Baños M, Villalonga ML, Villalonga R. 2002a. Stabilization of trypsin by chemical modification with β-cyclodextrin monoaldehyde. Biotechnol Lett 24:1455-1459.
6. Fernández M, Fragoso A, Cao R, Baños M, Villalonga R. 2002b. Stabilization of trypsin by chemical conjugation with monoamine derivatives of cyclodextrins. Enzyme Microb Technol 31:543-548.
7. Gekko K, Timasheff SN. 1981. Thermodynamic and kinetic examination of protein stabilization by glycerol. Biochemistry 20:4677-4686.
8. Gómez L, Villalonga R. 2000. Functional stabilization of invertase by covalent modification with pectin. Biotechnol Lett 22:1191-1195.
9. Kaneko T, Hamamoto T, Horikoshi K. 1988. Molecular cloning and nucleotide sequence of the cyclomaltodextrin glucanotransferase gene from alkalophilic Bacillus sp. strain no. 38-2. J Gen Microbiol 139:97-105.
10. Kazan D, Erarslan A. 1999. Effect of dextran polymers on the stability of soluble Escherichia coli penicillin G acylase. J Chem Technol Biotechnol 74:1157-1164.
11. Laskowski M. 1955. Trypsinogen and trypsin. Methods Enzymol 2:26-36.
12. Longo MA, Combes D. 1999. Thermostability of modified enzymes: a detailed study. J Chem Technol Biotechnol 74:25-32.
13. Lozano P, Combes D, Iborra JL. 1994. Effect of polyols on α-chymotrypsin thermostability: a mechanistic analysis of the enzyme stabilization. J Biotechnol 35:9-18.
14. Mateo C, Abian O, Fernandez-Lafuente R, Guisan JM. 2000. Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment. Enzyme Microb Technol 26:509-515.
15. Minagawa H, Kaneko H. 2000. Effect of double mutation on thermostability of lactate oxidase. Biotechnol Lett 22:1131-1133.
16. Pace NC, Barrett A. 1984. Kinetics of tryptic hydrolysis of the arginine-valine bond in folded and unfolded ribonuclease TI. Biochem J 219:411-417.
17. Rajalakshmi N, Sundaram PV. 1995. Stability of native and covalently modified papain. Protein Eng 8:1039-1047.
18. Sadana A, Henley JP. 1987. Single-step unimolecular non-first-order enzyme deactivation. Biotechnol Bioeng 30:717-723.
19. Schneider HJ, Xiao F. 1992. Binding and catalysis with a metal-induced ternary complex of an ethylenediamine-substituted cyclodextrin. J Chem Soc Perkin Trans 2 387-391.
20. Schwert GB, Takenaka Y. 1955. A spectrophotometric determination of trypsin and chymotrypsin. Biochim Biophys Acta 16:570-575.
21. Szejtli J. 1998. Introduction and general overview of cyclodextrins. Chem Rev 98:1743-1754.
22. Villalonga R, Fernández M, Fragoso A, Cao R, Di Pierro P, Mariniello L, Porta R. 2003. Transglutaminase-catalyzed synthesis of trypsin-cyclodextrin conjugates. Kinetics and stability properties. Biotechnol Bioeng 81:732-737.
23. Villalonga R, Villalonga ML, Gómez L. 2000. Preparation and functional properties of trypsin modified by carboxymethylcellulose. J Mol Catal B Enzymatic 10:483-490.
24. Zhong N, Byun HS, Bittman R. 1998. A convenient method for the monotosylation of β-cyclodextrin on the primary side. Tetrahedron Lett 38:2919-2920.