Involvement of two basic residues (Lys-270 and Arg-276) of human liver 3α-hydroxysteroid dehydrogenase in NADP(H) binding and activation by sulphobromophthalein: Site-directed mutagenesis and kinetic analysis

Biochemical Journal

Volumn 322, Issue 1, 1997, Pages 89-93

  Matsuura, Kazuya ^a   Tamada, Yoshiyuki ^a   Sato, Kumiko ^a   Iwasa, Harunori ^a   Miwa, Gunpei ^a   Deyashiki, Yoshihiro ^a   Hara, Akira ^a  

^a GIFU PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

3ALPHA HYDROXYSTEROID DEHYDROGENASE; ALCOHOL; ARGININE; BROMSULFOPHTHALEIN; LIVER ENZYME; LYSINE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; ENERGY; ENZYME ACTIVATION; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; GENETIC CONSERVATION; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

3-HYDROXYSTEROID DEHYDROGENASES; ARGININE; BINDING SITES; ENZYME ACTIVATION; HUMANS; KINETICS; LIVER; LYSINE; MUTAGENESIS, SITE-DIRECTED; NADP; SULFOBROMOPHTHALEIN;

EID: 0031058232     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3220089     Document Type: Article

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