Determinants of cofactor specificity in isocitrate dehydrogenase: Structure of an engineered NADP+ → NAD+ specificity-reversal mutant

Biochemistry

Volumn 35, Issue 18, 1996, Pages 5670-5678

  Hurley, James H ^a   Chen, Ridong ^b   Dean, Antony M ^b  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ISOCITRATE DEHYDROGENASE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CRYSTALLOGRAPHY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENETIC ENGINEERING; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; STEREOCHEMISTRY;

AMINO ACID SEQUENCE; BINDING SITES; COENZYMES; DRUG DESIGN; ESCHERICHIA COLI; ISOCITRATE DEHYDROGENASE; KINETICS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; NAD; NADP; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI;

EID: 0029886080     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi953001q     Document Type: Article

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