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Volumn 41, Issue 20, 2002, Pages 6226-6236

Optimizing an artificial metabolic pathway: Engineering the cofactor specificity of Corynebacterium 2,5-diketo-D-gluconic acid reductase for use in vitamin C biosynthesis

(5) Banta, Scott a,b Swanson, Barbara A c,d Wu, Shan c Jarnagin, Alisha c Anderson, Stephen b,e

a The State University of New Jersey (United States)

b RUTGERS UNIVERSITY (United States)

c GENENCOR INTERNATIONAL INC (United States)

d Applied Molecular Evolution (United States)

e RUTGERS UNIVERSITY (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COFACTOR;

ASSAYS; BIOSYNTHESIS; ENZYME KINETICS; ENZYMES; GELS; MUTAGENESIS; MUTAGENS; OPTIMIZATION;

VITAMINS;

2,5 DIKETO DEXTRO GLUCONIC ACID REDUCTASE; ASCORBIC ACID; OXIDOREDUCTASE; POLYACRYLAMIDE GEL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; BIOSYNTHESIS; CATALYST; CORYNEBACTERIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; KINETICS; METABOLISM; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION;

ALANINE; AMINO ACID SUBSTITUTION; ASCORBIC ACID; BINDING SITES; CORYNEBACTERIUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; GLUCONATES; GLYCINE; KINETICS; MUTAGENESIS, SITE-DIRECTED; NAD; NADP; PHENYLALANINE; RECOMBINANT FUSION PROTEINS; SUGAR ALCOHOL DEHYDROGENASES; THERMODYNAMICS; TYROSINE;

ACTINOBACTERIA (CLASS); CORYNEBACTERIUM; UNCULTURED ACTINOMYCETE;

EID: 0037150104 PISSN: 00062960 EISSN: None Source Type: Journal
DOI: 10.1021/bi015987b Document Type: Article

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