Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres

Nature Cell Biology

Volumn 7, Issue 1, 2005, Pages 42-47

  Maiato, Helder ^a   Khodjakov, Alexey ^a,b,c   Rieder, Conly L ^a,b,c  

^a WADSWORTH CENTER   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TUBULIN; DROSOPHILA PROTEIN; GREEN FLUORESCENT PROTEIN; PROTEIN CLASP; PROTEIN SUBUNIT; RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BLEACHING; CELL TRANSPORT; CENTROMERE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; INSECT CELL; LASER SURGERY; MICROSURGERY; MICROTUBULE; NONHUMAN; ORTHOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TRANSPORT; RNA INTERFERENCE;

ANIMALS; CELL LINE; CHROMOSOME SEGREGATION; DOWN-REGULATION; DROSOPHILA; DROSOPHILA PROTEINS; GREEN FLUORESCENT PROTEINS; KINETOCHORES; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MITOSIS; MITOTIC SPINDLE APPARATUS; MODELS, BIOLOGICAL; PHOTOBLEACHING; RNA INTERFERENCE; TUBULIN;

ANIMALIA; DROSOPHILA MELANOGASTER; INSECTA; MELANOGASTER;

EID: 12344300350     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb1207     Document Type: Article

References (29)

1. Mitchison, T. J. Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J. Cell Biol. 109, 637-652 (1989).
2. Rogers, G. C. et al. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature 427, 364-370 (2004).
3. Carvalho, P., Tirnauer, J. S. Pellman, D. Surfing on microtubule ends. Trends Cell Biol. 13, 229-237 (2003).
4. Lemos, C. L. et al. Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organi ation. EMBO J. 19, 3668-3682 (2000).
5. Maiato, et al. uman CLASP1 is an outer kinetochore component that regulates spindle microtubule dyanmics. Cell 113, 891-904 (2003).
6. Maiato, et al. MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. J. Cell Biol. 157, 749-760 (2002).
7. Goshima, G. ale, R. D. The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line. J. Cell Biol. 162, 1003-1016 (2003).
8. Maiato, Rieder, C. L. Khodjakov, A. Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during mitosis in animals. J. Cell Biol. DOI: 10.1083/jcb.200407090.
9. Ganem, N.J. Compton, D.A. The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK. J. Cell Biol. 166, 473-478 (2004).
10. Lawrence, C. J. et al. A standardi ed kinesin nomenclature. J. Cell Biol. 167, 19-22 (2004).
11. Yin, You, L., Pasqualone, D., Kopski, K. M. uffaker, T. C. Stu1p is physically associated with β-tubulin and is required for structural integrity of the mitotic spindle. Mol. Biol. Cell 13, 1881-1892 (2002).
12. Sharp, D. J. MAST sails through mitosis. Curr. Biol. 12, R585-R587 (2002).
13. Desai, A. et al. KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev. 17, 2421-2435 (2003).
14. offman, D. B., Pearson, C. G., Yen, T. J., owell, B. J. Salmon, E. D. Microtubule dependent changes in the assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at kinetochores. Mol. Biol. Cell 12, 1995-2009 (2001).
15. Tirnauer, J. S., Canman, J. C., Salmon, E. D. Mitchison, T. J. EB1 targets to kinetochores with attached, polymeri ing microtubules. Mol. Biol. Cell 13, 4308-4316 (2002).
16. Rogers, S. L., Rogers, G. C., Sharp, D. J. ale, R. D. Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle. J. Cell Biol. 158, 873-884 (2002).
17. Waters, J. C., Mitchison, T. J., Rieder, C. L. Salmon, E. D. The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work. Mol. Biol. Cell 7, 1547-1558 (1996).
18. Caban, B. B., Forer, A. Bajer, A. S. Ultraviolet microbeam irradiation of chromosomal spindle fibres in Haemanthus katherinae endosperm. I. Behaviour of the irradiated region. J. Cell Sci. 105, 571-578 (1993).
19. Forer, A., Spurck, T. Pickett-eaps, J. Ultaviolet microbeam irradiations of spindle fibers in crane-fly spermatocytes and newt epithelial cells: resolution of previously conflicting observations. Protoplasma 197, 230-240 (1997).
20. Gordon, G. W. The Control of Mitotic Motility as Influenced by Ultraviolet Microbeam Irradiation of Kinetochore Fibers. Thesis, Univ. Pennsylvania (1980).
21. Wadsworth, P. Khodjakov, A. E pluribus unum: towards a universal mechanism for spindle assembly. Trends Cell Biol. 14, 413-419 (2004).
22. Chen, W. hang, D. Kinetochore fibre dynamics outside the contex of the spindle during anaphase. Nature Cell Biol. 6, 227-231 (2004).
23. LaFountain, J. R. Jr., Cohan, C. S., Siegel, A. J. LaFountain, D. J. Direct visualiation of microtubule flux during metaphase and anaphase in crane-fly spermatocytes. Mol. Biol. Cell 15, 5724-5732 (2004).
24. Mitchison, T. J. Salmon, E. D. Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J. Cell Biol. 119, 569-582 (1992).
25. Brust-Mascher, I. Scholey, J. M. Microtubule flux and sliding in mitotic spindles of Drosophila embryos. Mol. Biol. Cell 13, 3967-3975 (2002).
26. Maddox, P., Desai, A., Oegema, K., Mitchison, T. J. Salmon, E. D. Poleward microtubule flux is a major component of spindle dynamics and anaphase A in mitotic Drosophila embryos. Curr. Biol. 12, 1670-1674 (2002).
27. Skibbens, R., Skeen, P. Salmon, E. D. Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism. J. Cell Biol. 122, 859-875 (1993).
28. Khodjakov, A. Rieder, C. L. Kinetochores moving away from their associated pole do not exert a significant pushing force on the chromosome. J. Cell Biol. 135, 315-327 (1996).
29. Maiato, Sunkel, C. E. Earnshaw, W. C. Dissecting mitosis by RNAi in Drosophila tissue culture cells. Biol. Proced. 5, 153-161 (2003).