Stu1p is physically associated with β-tubulin and is required for structural integrity of the mitotic spindle

Molecular Biology of the Cell

Volumn 13, Issue 6, 2002, Pages 1881-1892

  Yin, Hongwei ^a   You, Liru ^a   Pasqualone, Danielle ^a   Kopski, Kristen M ^a   Huffaker, Tim C ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN; DNA; KINESIN; MICROTUBULE ASSOCIATED PROTEIN; PRIMER DNA; REPRESSOR PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; STU1 PROTEIN, S CEREVISIAE; TUBULIN;

ARTICLE; BINDING AFFINITY; GENE OVEREXPRESSION; MITOSIS SPINDLE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE SENSITIVE MUTANT; YEAST; CHEMICAL STRUCTURE; CHEMISTRY; CYTOLOGY; GENETIC TRANSCRIPTION; GENETICS; GENOTYPE; METABOLISM; MUTAGENESIS; PHYSIOLOGY; PROTEIN CONFORMATION; PROTEIN SYNTHESIS; ULTRASTRUCTURE;

BASE SEQUENCE; DNA PRIMERS; GENOTYPE; MICROTUBULE-ASSOCIATED PROTEINS; MITOTIC SPINDLE APPARATUS; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN CONFORMATION; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC; TRANSLATION, GENETIC; TUBULIN; PROTEIN BIOSYNTHESIS;

EID: 0035985166     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.01-09-0458     Document Type: Article

References (45)

1. Dominant suppressors of yeast actin mutations that are reciprocally suppressed
2. Clasps are clip-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts
3. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
4. It's a kar9ochore to capture microtubules
5. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
6. Dynein and dynactin are localized to astral microtubules and at cortical sites in mitotic epithelial cells
7. Preparation of yeast cells for thin-section electron microscopy
8. Randomization of genes by PCR mutagenesis
9. The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly
10. Yeast-enhanced green fluorescent protein (yEGFP) a reporter of gene expression in Candida albicans
11. Three-dimensional reconstruction and analysis of mitotic spindles from the yeast Schizosaccharomyces pombe
12. Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin
13. The structure of an actin-crosslinking domain from human fimbrin
14. Identification of a novel microtubule binding and assembly domain in the developmentally regulated inter-repeat region of tau
15. Flow cytometric analysis of DNA content in budding yeast
16. The kinesin-like protein KLP61F is essential for mitosis in Drosophila
17. Mapping actin surfaces required for functional interactions in vivo
18. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly
19. Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster
20. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5
21. A bipolar kinesin
22. Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization
23. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
24. The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
25. Interpolar spindle microtubules in PTK cells
26. Kinetochore microtubules in Ptk cells
27. Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset
28. A rapid method for localized mutagenesis of yeast genes
29. STU1, a suppressor of a β-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle
30. Systematic mutational analysis of the yeast β-tubulin gene
31. Structure-function relationships in yeast tubulins
32. Allele-specific suppression by formation of new protein-protein interactions in yeast
33. Kinesin-related proteins required for structural integrity of the mitotic spindle
34. RASMOL: Biomolecular graphics for all
35. The bipolar kinesin, K.L.P61F., cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles
36. Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos
37. Getting started with yeast
38. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
39. The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos
40. Motoring to the finish: Kinesin and dynein work together to orient the yeast mitotic spindle [comment]
41. Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body components
42. Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body
43. Systematic mutational analysis of the yeast ACT1 gene
44. Three- dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle
45. The spindle: A dynamic assembly of microtubules and motors