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Volumn 4, Issue 1, 2004, Pages 51-54

Direct Ultrasensitive Electrical Detection of DNA and DNA Sequence Variations Using Nanowire Nanosensors

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PEPTIDE NUCLEIC ACID; RECEPTOR; SILICON;

EID: 0842308906     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl034853b     Document Type: Article
Times cited : (1267)

References (27)
  • 8
    • 0141521706 scopus 로고    scopus 로고
    • Chen, R. J.; Bangsaruntip, S.; Drouvalakis, K. A.; Kam, N. W. S.; Shim, M.; Li, Y.; Kim, W.; Utz, P. J.; Dai, H. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 4984. Star, A.; Gabriel, J.-C. P.; Bradley, K.; Gruner, G. Nano Lett. 2003, 3, 459.
    • (2003) Nano Lett. , vol.3 , pp. 459
    • Star, A.1    Gabriel, J.-C.P.2    Bradley, K.3    Gruner, G.4
  • 17
    • 0842319624 scopus 로고    scopus 로고
    • Cystic Fibrosis Foundation, http://www.cff.org.
  • 20
    • 0842276466 scopus 로고    scopus 로고
    • note
    • 6 ratio of 4000:1 during growth. SiNWs with an average diameter of 20 nm were suspended in ethanol and deposited on oxidized Si substrates. Electrical contacts were made to NW ends using electron-beam lithography, followed by Ti/Au (50/50 nm) metallization. Linear current (I) versus voltage (V) behavior was observed for all devices, and these devices had conductance values between 200 and 1700 nS.
  • 21
    • 0842319626 scopus 로고    scopus 로고
    • note
    • 2 flow rate of 250 sccm, Anatech Ltd., SP100, Springfield, VA). A PDMS microfluidic channel was fabricated using a standard photolithography with channel dimensions of 500 μm x 3 mm. Biotinyl p-nitrophenyl ester, pyridine (99.9%), and 4-(dimethylamino)pyridine (DMAP) were purchased from Aldrich (St. Louis, MO). Immediately after the plasma cleaning, SiNWs were incubated overnight at room temperature in 10 mL pyridine solution containing 10 mg of biotinyl p-nitrophenyl ester and 5 mg of DMAP. SiNWs were subsequently rinsed three times with pyridine and the microfluidic channel was placed on the device for further NW surface modification and sample introduction. A 30 μg/mL avidin solution in PBS, pH 7, was introduced for 30 min to terminate the NW surface with avidin. The avidin-modified NW was further linked with biotinylated PNA probes by adding 100 μmol of PNA capture probes for 30 min.
  • 24
    • 0842319623 scopus 로고    scopus 로고
    • note
    • PNA probes with WT base sequence of ΔF508 in the CFTR gene, 5′-ATCATCTTTG-3′, were purchased from PE Applied Biosystems (Branchburg, NJ) and resuspended in distilled water. The capture PNA probes were biotinylated at the 5′ end. Single-stranded DNA pieces containing WT and MU ΔF508 in the CFTR gene, 5′-CATAGGAAACACCAAAGATGATATTTTCTTT- 3′ and 5′-CATAGGAAACACCAATGATATTTTCTTT-3′ respectively, were obtained from Operon Technologies Inc. (Qiagen, San Francisco, CA). The oligonucleotides were reconstituted in nuclease-free water (Promega, Milwaukee, WI) at pH 7.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.