Tight Binding of Deoxyribonucleotide Triphosphates to Human Thymidine Kinase 2 Expressed in Escherichia coli. Purification and Partial Characterization of Its Dimeric and Tetrameric Forms

Biochemistry

Volumn 42, Issue 51, 2003, Pages 15158-15169

  Barroso, João Filipe ^a   Elholm, Morten ^a,b   Flatmark, Torgeir ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGGLOMERATION; DIMERS; ESCHERICHIA COLI; ETHANOL; PURIFICATION;

MOLECULAR DOCKING;

ENZYMES;

ALCOHOL; CHAPERONIN; DEOXYRIBONUCLEOTIDE TRIPHOSPHATE DERIVATIVE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; NUCLEOSIDE PHOSPHATE; NUCLEOSIDE TRIPHOSPHATE; PHOSPHATE; PYRIMIDINE DERIVATIVE; THYMIDINE KINASE; THYMIDINE KINASE 2; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CALCULATION; CONTROLLED STUDY; DIMERIZATION; ENERGY; ENZYME BINDING; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; ESCHERICHIA COLI; FEEDBACK SYSTEM; HEAT SHOCK RESPONSE; KINETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; STOICHIOMETRY; TEMPERATURE DEPENDENCE;

BINDING SITES; CELL LINE, TUMOR; CLONING, MOLECULAR; CULTURE MEDIA, CONDITIONED; DEOXYADENINE NUCLEOTIDES; DEOXYCYTOSINE NUCLEOTIDES; DIMERIZATION; ENZYME INHIBITORS; ESCHERICHIA COLI; ETHANOL; GROEL PROTEIN; GROES PROTEIN; HUMANS; ISOENZYMES; KINETICS; MODELS, MOLECULAR; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; THYMIDINE KINASE; THYMINE NUCLEOTIDES; TRYPTOPHAN;

ESCHERICHIA COLI;

EID: 0348224028     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035230f     Document Type: Article

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