Chronology of Cellular Alterations during 7-Ketocholesterol-Induced Cell Death on A7R5 Rat Smooth Muscle Cells: Analysis by Time Lapse-Video Microscopy and Conventional Fluorescence Microscopy

Cytometry Part A

Volumn 52, Issue 2, 2003, Pages 57-69

  Zahm, Jean Marie ^a   Baconnais, Sonia ^a   Monier, Serge ^b   Bonnet, Noël ^a   Bessède, Ginette ^b   Gambert, Philippe ^b   Puchelle, Edith ^a   Lizard, Gérard ^b  

^a INSERM   (France)

^b HÔPITAL DU BOCAGE   (France)

Author keywords

7 ketocholesterol; A7R5 rat smooth muscle cells; Actin; Cell death; Mitochondria; Time lapse video microscopy

Indexed keywords

7 OXOCHOLESTEROL; ACTIN; FLUORESCEIN; HOE 33342; PHALLOIDIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL DEATH; CELL SHAPE; CONTROLLED STUDY; CYTOSKELETON; EMBRYO; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; FLUORESCENCE TIME LAPSE VIDEO MICROSCOPY; MEMBRANE POTENTIAL; MICROSCOPY; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT; THORACIC AORTA; TIME LAPSE VIDEO MICROSCOPY; VASCULAR SMOOTH MUSCLE; VIDEORECORDING;

EID: 0346244108     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10027     Document Type: Article

References (68)

1. Addis PB. Occurence of lipid oxidation products in foods. Food Chem Toxicol 1986;24:1021-1030.
2. Smith L. Review of progress in sterol oxidations: 1987-1995. Lipids 1996;31:453-457.
3. Schroepfer GJ. Oxysterols: modulators of cholesterol metabolism and other processes. Physiol Rev 2000;80:361-554.
4. Wolf G. The role of oxysterols in cholesterol homeostasis. Nutr Rev 1999;57:196-198.
5. Best PJM, Hasdai D, Sangiorgi G, Schwartz RS, Holmes DR, Simari RD, Lerman A. Basic concepts and implications in coronary artery disease. Arterioscler Thromb Vasc Biol 1999;19:14-22.
6. Vaya J, Aviram M, Mahmood S, Hayek T, Grenadir E, Hoffman A, Milo S. Selective distribution of oxysterols in atherosclerotic lesions and human plasma lipoproteins. Free Radic Res 2001;34:485-497.
7. Ziedèn B, Kaminskas A, Kristenson M, Kucinskienenê Z, Vessby B, Olsson AG, Diczfalusy U. Increased plasma 7β-hydroxycholesterol concentrations in a population with a high risk for cardiovascular disease. Arterioscler Thromb Vasc Biol 1999;19:967-971.
8. Zhou Q, Vasowicz E, Handler B, Fleischer L, Kummerow FA. An excess concentration of oxysterols in the plasma is cytotoxic to cultured endothelial cells. Atherosclerosis 2000;149:191-197.
9. Lemaire S, Lizard G, Monier S, Miguet C, Gueldry S, Volot F, Gambert P, Néel D. Different patterns of IL-1β secretion, adhesion molecule expression and apoptosis in human endothelial cells treated with 7α, 7β-hydroxycholesterol, or 7-ketocholesterol. FEBS Lett 1998;440:434-439.
10. Lizard G, Miguet C, Bessède G, Monier S, Gueldry S, Néel D, Gambert P. Impairment with various antioxidants of the loss of mitochondrial transmembrane potential and of the cytosolic release of cytochrome c occurring during 7-ketocholesterol-induced apoptosis. Free Radic Biol Med 2000;28:743-753.
11. Miguet-Alfonsi C, Prunet C, Monier S, Bessède G, Lemaire-Ewing S, Berthier A, Ménétrier F, Néel D, Gambert P, Lizard G. Analysis of oxidative processes and of myelin figures formation before and after the loss of mitochondrial transmembrane potential during 7β-hydroxycholesterol- and 7-ketocholesterol-induced apoptosis: comparison with various pro-apoptotic chemicals. Biochem Pharmacol 2002; 64:527-541.
12. Mügge A. The role of reactive oxygen species in atherosclerosis. Z Kardiol 1998;87:851-864.
13. Kaul D. Molecular link between cholesterol, cytokines and atherosclerosis. Mol Cell Biol 2001;219:65-71.
14. Clare K, Hardwick SJ, Carpenter KLH, Weeratunge N, Mitchinson MJ. Toxicity of oxysterols to human monocyte-macrophages. Atherosclerosis 1995;118:67-75.
15. Lizard G, Monier S, Cordelet C, Gesquière L, Deckert V, Gueldry S, Lagrost L, Gambert P. Characterization and comparison of the mode of cell death, apoptosis versus necrosis, induced by 7β-hydroxycholesterol and 7-ketocholesterol on the cells of the vascular wall. Arterioscler Thromb Vasc Biol 1999;19:1190-2000.
16. Yuan XM, Li W, Brunk, UT, Dalen H, Chang YH, Sevanian A. Lysosomal destabilization during macrophage damage induced by cholesterol oxidation products. Free Radic Biol Med 2000;28:208-218.
17. Bursch W. The autophagosomal-lysosomal compartment in programmed cell death. Cell Death Differ 2001;8:569-581.
18. Miguet-Alphonsi C. Oxystérols et apoptose-étude des mécanismes post-mitochondriaux et de la production de radicaux oxygénés au cours de la mort cellulaire induite par le cétocholestérol et par le 7β-hydroxycholestérol [thesis]. Burgundy, France: Department of Biochemistry, Cellular and Molecular Biology, University of Burgundy; 2002.
19. Radosevic K, van Leeuwen AMT, Segers-Nolten IMJ, Figdor CG, de Grooth BG, Greve J. Changes in actin organization during the cytotoxic process. Cytometry 1994;15:320-326.
20. Kahn E, Lizard G, Frouin F, Gueldry S, Bessède G, Gambert P, Todd-Pokropek A. Changes at the mitochondrial level during oxysterol-induced apoptosis. Cytometry 2002;suppl 11:78.
21. Suarez-Huerta N, Mosselmans R, Dumont JE, Robaye B. Actin depolymerization and polymerization are required during apoptosis in endothelial cells. J Cell Physiol 2000;184:239-245.
22. Coleman ML, Sahai EA, Yeo M, Bosch M, Dewar A, Olson MF. Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I. Nat Cell Biol 2001;3:339-345.
23. Palladini G, Finardi G, Bellomo G. Disruption of actin microfilament organization by cholesterol oxides in 73/73 endotlielial cells. Exp Cell Res 1996;223:27-82.
24. Kimes WB, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp Cell Res 1976;98:349-366.
25. Lizard G, Gueldry S, Sordet O, Monier S, Athias A, Miguet C, Bessède G, Lemaire S, Solary E, Gambert P. Glutathione is implied in the control of 7-ketocholesterol-induced apoptosis, which is associated with radical oxygen species production. FASEB J 1998;12:1651-1663.
26. Chen LB. Mitochondrial membrane potential in living cells. Annu Rev Cell Biol 1988;4:155-181;1988.
27. Macho A, Decaudin D, Castedo M, Hirsch T, Susin SA, Zamzami N, Kroemer G. Chloromethyl-X-rosamine is an aldehyde-fixable potential-sensitive fluorochrome for the detection of early apoptosis. Cytometry 1996;25:333-340.
28. Terasaki M, Song J, Wong JR, Reiss MJ, Chen LB. Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell 1984;38:101-108.
29. Ramoino P, Diaspro A, Fato M, Beltrame F, Robello M. Changes in the endoplasmic reticulum structure of Paramecium primaurelia in relation to different cellular physiological states. J Photochem Photobiol B Biol 2000;54:35-42.
30. Suzuki A, Endo T. Ermelin, an endoplasmic reticulum transmembrane protein, contains the novel HELP domain conserved in eucaryotes. Gene 2002;284:31-40.
31. Bereiter-Hahn J, Voth M. Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria. Microsc Res Tech 1994;27:198-219.
32. Gilmore K, Wilson M. The use of chloromethyl-X-rosamine (Mitotracker red) to measure loss of mitochondrial membrane potential in apoptotic cells is incompatible with cell fixation. Cytometry 1999;36:355-358.
33. Lizard G, Fournel S, Genestier L, Dhedin N, Chaput C, Flacher M, Mutin M, Panaye G, Revillard JP. Kinetics of plasma membrane and mitochondrial alterations in cells undergoing apoptosis. Cytometry 1995;21:275-283.
34. Zahm JM, Kaplan H, Hérard AL, Doriot F, Pierrot D, Somelette P, Puchelle E. Cell migration and proliferation during the in vitro wound repair of the respiratory epithelium. Cell Motil Cytoskeleton 1997;37:33-43.
35. De La Cruz E, Pollard TD. Transient kinetic analysis of rhodamine phalloidin binding to actin filaments. Biochemistry 1994;33:14387-14392.
36. Minamikawa T, Sriratana A, Williams DA, Bowser DN, Hill JS, Nagley P. Chloromethyl-X-rosamine (MitoTracker Red) photosensitizes mitochondria and induces apoptosis in intact human cells. J Cell Sci 1999;112:2419-2430.
37. Bonnet N, Zahm JM. Analysis of image sequences in fluorescence videomicroscopy of stationary objects. Cytometry 1998;31:217-228.
38. Bonnet N, Raby B, Zahm JM. Dimensionality reduction, segmentation and quantification of multi-dimensional images: application to fluorescence microscopy. Prog Biomed Optics Imaging 2000;1:140-150.
39. Lizard G, Moisant M, Cordelet C, Monier S, Gambert P, Lagrost L. Induction of similar features of apoptosis in human and bovine vascular endothelial cells treated by 7-ketocholesterol. J Pathol 1997;183:330-338.
40. Miyashita Y, Ozaki H, Koide N, Otsuka M, Oyama T, Itoh Y, Mastuzaka T, Shirai K. Oxysterol-induced apoptosis of vascular smooth muscle cells is reduced by HMG-CoA reductase inhibitor, pravastatin. J Atheroscler Thromb 2002;9:65-71.
41. Loewenstein WR. Junctional intercellular communication and the control of cell growth. Biochim Biophys Acta 1979;560:1-65.
42. Zwijsen RM, Oudenhoven IM, de Haan LH. Effects of cholesterol and oxysterols on gap junctional communication between human smooth muscle cells. Eur J Pharmacol 1992;228:115-120.
43. Berliner JA, Heinecke JW. The role of oxidized lipoproteins in atherogenesis. Free Radic Biol Med 1996;20:707-727.
44. Rao JY, Jin YS, Zheng Q, Cheng J, Tai J, Hemstreet GP. Alterations of the actin polymerization status as an apoptotic morphological effector in HL-60 cells. J Cell Biochem 1999;75:686-697.
45. Lum H, Roebuck KA. Oxidative stress and endothelial cell dysfunction. Am J Physiol Cell Physiol 2001;280:C719-C741.
46. Kayalar C, Ord T, Testa MP, Zhong LT, Bredesen DE. Cleavage of actin by interleukin 1β-converting enzyme to reverse DNase I inhibition. Proc Natl Acad Sci USA 1996;93:2234-2238.
47. Lemasters JJ, Nieminen AL, Qian T, Trost LC, Elmore SP, Nishimura Y, Crowe RA, Cascio WE, Bradham CA, Brenner DA, Herman B. The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy. Biochim Biophys Acta 1998;1366:177-196.
48. Sun XM, Snowden RT, Skilleter DN, Dindsdale D, Ormerod MG, Cohen GM. A flow cytometric method for the separation and quantitation of normal and apoptotic thymocytes. Anal Biochem 1992;204:351-356.
49. Kahn E, Lizard G, Monier S, Bessède G, Frouin F, Gambert P, Todd-Pokropek A. Evaluation by flow cytometry and by factor analysis of confocal image sequences of morphological and functional changes occurring at the mitochondrial level during 7-ketocholesterol-induced cell death. Anal Quant Cytol Histol 2002;24:355-362.
50. Farkas DL, Wei MD, Febbroriello P, Carson JH, Loew LM. Simultaneous imaging of cell and mitochondrial membrane potentials. Biophys J 1989;56:1053-1069.
51. Funk RH, Nagel F, Wonka F, Krinke HE, Golfert F, Hofer A. Effects of heat shock on the functional morphology of cell organelles observed by video-enhanced microscopy. Anat Rec 1999;255:458-464.
52. Rocchi E, Vigo J, Viallet P, Bonnard I, Salmon JM. Multiwavelength video microfluorometric study of cytotoxic effects of a marine peptide, didemnin B, on normal and MDR resistant CCRF-CEM cell lines. Anticancer Res 2000;20:987-996.
53. Margineantu D, Capaldi RA, Marcus AH. Dynamics of the mitochondrial reticulum in live cells using Fourier imaging correlation spectroscopy and digital video microscopy. Biophys J 2000;79:1833-1849.
54. Gao W, Pu Y, Luo KQ, Chang DC. Temporal relationship between cytochrome c release and mitochondrial swelling during UV-induced apoptosis in living HeLa cells. J Cell Sci 2001;114:2855-2862.
55. Desagher S, Martinou JC. Mitochondria as central control point of apoptosis. Trends Cell Biol 2000;10:369-377.
56. Narita M, Shimizu S, Ito T, Chittenden T, Lutz RJ, Matsuda H, Tsujimoto Y. Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria. Proc Natl Acad Sci USA 1998;95:14681-14686.
57. Vander Heiden MG, Chandel NS, Williamson EK, Schumacker PT, Thompson CB. Bcl-xL regulates the membrane potential and volume homeostasis. Cell 1997;91:627-637.
58. Karbowski M, Kurono C, Wozniak M, Ostrowski M, Teranishi M, Nishizawa Y, Usukura J, Soji T, Wakabayashi T. Free radical-induced megamitochondria formation and apoptosis. Free Radic Biol Med 1999;26:396-409.
59. Vander Heiden MG, Thompson CB. Bcl-2 proteins: regulators of apoptosis or of mitochondrial homeostasis? Nat Cell Biol 1999;1:E209-E216.
60. Desagher S, Martinou JC. Mitochondria as the central control point of apoptosis. Trends Cell Biol 2000;10:369-377.
61. Métivier D, Dallaporta B, Zamzami N, Larochette N, Susin SA, Marzo I, Kroemer G. Cytofluorimetric detection of mitochondrial alterations in early CD95/Fas/APO-1-triggered apoptosis of Juckat T lymphoma cells. Comparison of seven mitochondrion-specific fluorochromes. Immunol Lett 1998;61:157-163.
62. Kroemer G, Reed JC. Mitochondrial control of cell death. Nat Med 2000;6:513-519.
63. Sanchez-Alcazar JA, Ault JG, Khodjakov A, Schneider E. Increased mitochondrial cytochrome c levels and mitochondrial hyperpolarization precede camptothecin-induced apoptosis in Jurkat cells. Cell Death Differ 2000;7:1090-1100.
64. Poppe M, Reimertz C, Dussmann H, Krohn AJ, Luetjens CM, Bockelmann D, Nieminen AL, Kogel D, Prehn JH. Dissipation of potassium and proton gradient inhibits mitochondrial hyperpolarization and cytochrome c release during neural apoptosis. J Neurosci 2001;21:4551-4563.
65. Pham NA, Hedley DW. Respiratory chain-generated oxidative stress following treatment of leukemic blasts with DNA-damaging agents. Exp Cell Res 2001;264:345-352.
66. Krysko DV, Roels F, Leybaert L, D'Herde K. Mitochondrial transmembrane potential changes support the concept of mitochondrial heterogeneity during apoptosis. J Histochem Cytochem 2001;49:1277-1284.
67. Hengartner MO. The biochemistry of apoptosis. Nature 2000;407:770-776.
68. Green D, Kroemer G. The central execution of apoptosis: caspases or mitochondria? Trends Cell Biol 1998;8:267-271.