Effects of heat shock on the functional morphology of cell organelles observed by video-enhanced microscopy

Anatomical Record

Volumn 255, Issue 4, 1999, Pages 458-464

  Funk, R H W ^a,b   Nagel, F ^a   Wonka, F ^a   Krinke, H E ^a   Golfert F ^a   Hofer, A ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

Author keywords

Astrocytes; Electronic light microscopy; Heat shock; MDCK cells; Microperfusion chamber; Mitochondria; Video enhanced contrast microscopy

Indexed keywords

ANIMAL CELL; ARTICLE; CARBON DIOXIDE TENSION; CELL CULTURE; CELL STRUCTURE; ELECTRON MICROSCOPY; HEAT SHOCK; LASER MICROSCOPY; MITOCHONDRION SWELLING; NONHUMAN; OXYGEN TENSION; PH; PRIORITY JOURNAL; RAT; TEMPERATURE;

ANIMALS; ASTROCYTES; CELL LINE; CELLS, CULTURED; DOGS; HEAT; MEMBRANE POTENTIALS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; MICROSCOPY, VIDEO; MITOCHONDRIA; MITOCHONDRIAL SWELLING; ORGANELLES; PERFUSION; RATS; STAINING AND LABELING;

ANIMALIA;

EID: 0033179089     PISSN: 0003276X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0185(19990801)255:4<458::AID-AR11>3.0.CO;2-U     Document Type: Article

References (25)

1. Collier NC, Sheetz MP, Schlesinger MJ. 1993. Concomitant changes in mitochondria and intermediate filaments during heat shock and recovery of chicken embryo fibroblasts. J Cell Biochem 52:297-307.
2. Cossarizza A, Baccarani-Contri M, Kalashnikova G, Franceschi C. 1993. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′- tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem Biophys Res Commun 197:40-45.
3. Cossarizza A, Cooper EL, Quaglino D, Salvioli S, Kalachnikova G, Franceschi C. 1995. Mitochondrial mass and membrane potential in coelomocytes from the earthworm Eisenia foetida: studies with fluorescent probes in single intact cells. Biochem Biophys Res Commun 214:503-510.
4. Cossarizza A, Ceccarelli D, Masini A. 1996. Functional heterogeneity of an isolated mitochondrial population revealed by cytofluorometric analysis at the single organelle level. Exp Cell Res 222: 84-94.
5. Dermietzel R, Hertzberg EL, Kessler JA, Spray DC. 1991. Gap junctions between cultured astrocytes: immunocytochemical, molecular, and electrophysiological analysis. J Neurosci 11:1421-1432.
6. Di Lisa F, Blank PS, Colonna R, Gambassi G, Silverman HS, Stern MD, Hansford RG. 1995. Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition. J Physiol (Lond) 486:1-13.
7. Dubinsky JM, Levi Y. 1998. Calcium-induced activation of the mitochondrial permeability transition in hippocampal neurons [In Process Citation], J Neurosci Res 53:728-741.
8. L prevents cell death following growth factor withdrawal by facilitating mitochondrial ATP/ADP exchange. Mol Biol Cell 9 (Suppl):381a.
9. Hofer A, Nagel F, Wonka F, Krinke HE, Gšlfert F, Funk RHW. 1999. A new perfusable microchamber system for determination of cellular stress effects using video-enhanced microscopy during and after heat shock. Med Biol Eng Comput (in press).
10. Huckriede A, Heikema A, Sjollema K, Briones P, Agsteribbe E. 1995. Morphology of the mitochondria in heat shock protein 60 deficient fibroblasts from mitochondrial myopathy patients. Effects of stress conditions. Virchows Arch 427:159-165.
11. Huser J, Rechenmacher CE, Blatter LA. 1998. Imaging the permeability pore transition in single mitochondria. Biophys J 74:2129-2137.
12. Lai YK, Lee WC, Hu CH, Hammond GL. 1996. The mitochondria are recognition organelles of cell stress. J Surg Res 62:90-94.
13. Mancini M, Sedghinasab M, Knowlton K, Tam A, Hockenbery D, Anderson BO. 1998. Flow cytometric measurement of mitochondrial mass and function: a novel method for assessing chemoresistance. Ann Surg Oncol 5:287-295.
14. Matsuhashi T, Karbowski M, Liu X, Usukura J, Wozniak M, Wakabayashi T. 1998. Complete suppression of ethanol-induced formation of megamitochondria by 4-hydroxy-2,2,6,6-tetramethyl-piperidine-1-oxyl (4-OH-TEMPO). Free Radic Biol Med 24:139-147.
15. Mignotte B, Vayssière J-L. 1998. Mitochondria and apoptosis. Eur J Biochem 252:1-15.
16. Minamikawa T, Williams DA, Bowser DN, Nagley P. 1999. Mitochondrial permeability transition and swelling can occur reversibly without inducing cell death in intact human cells. Exp Cell Res 246:26-37.
17. Morara II, Mannella CA, Slepchenko B, Loew LM. 1998. Imaging pH in single mitochondria of living cells. Mol Biol Cell 9(Suppl):102a.
18. Murphy RC, Moodie A, Schneider E, Mannella CA, Campo ML, Kinnally KW. 1998. Investigations of the role of the protein translocating channels of mitochondria in apoptosis. Mol Biol Cell 9(Suppl): 244a.
19. Petit PX, Zamzami N, Vayssiere JL, Mignotte B, Kroemer G, Castedo M. 1997. Implication of mitochondria in apoptosis. Mol Cell Biochem 174:185-188.
20. Reers M, Smith TW, Chen LB. 1991. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 30:4480-4486.
21. Salvioli S, Ardizzoni A, Franceschi C, Cossarizza A. 1997. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett 411:77-82.
22. Tandler B, Horne WI, Brittenham GM, Tsukamoto H. 1996. Giant mitochondria induced in rat pancreatic exocrine cells by ethanol and iron. Anat Rec 245:65-75.
23. Vayssière JL, Petit PX, Risler Y, Mignotte B. 1994. Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40. Proc Natl Acad Sci USA 91:11752-11756.
24. Welch WJ, Suhan JP. 1985. Morphological study of the mammalian stress response: characterization of changes in cytoplasmic organelles, cytoskeleton, and nucleoli, and appearance of intranuclear actin filaments in rat fibroblasts after heat-shock treatment. J Cell Biol 101:1198-1211.
25. Zamzami N, Marchetti P, Castedo M, Zanin C, Vayssiere JL, Petit PX, Kroemer G. 1995. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J Exp Med 181:1661-1672.