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Volumn 75, Issue 21, 2003, Pages 5936-5943

Femtomolar Detection of Prostate-Specific Antigen: An Immunoassay Based on Surface-Enhanced Raman Scattering and Immunogold Labels

Author keywords

[No Author keywords available]

Indexed keywords

BIOSELECTIVITY;

EID: 0242354089     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac034356f     Document Type: Article
Times cited : (785)

References (65)
  • 1
    • 0004213320 scopus 로고    scopus 로고
    • Diamandis, E. P., Christopoulos, T. K., Eds.; Academic Press: New York
    • Immunoassay; Diamandis, E. P., Christopoulos, T. K., Eds.; Academic Press: New York, 1996.
    • (1996) Immunoassay
  • 53
    • 0242273847 scopus 로고    scopus 로고
    • McCreery, R. L. http://chemistry.ohio-state.edu/-rmccreer/freqcorr/images/nap.html.
    • McCreery, R.L.1
  • 62
    • 0242368651 scopus 로고    scopus 로고
    • note
    • Recall that the capture antibody was anti-free PSA while the antigen was serum PSA containing only 10-30% free PSA. The projected detection limit estimates are, however, based on the total PSA concentration of the antigen standard and therefore the detection limits for free PSA should be somewhat lower.
  • 63
    • 0242337184 scopus 로고    scopus 로고
    • note
    • Our past work16 reported a limit of detection of ∼30 ng/mL for an assay using polyclonal antibodies. The ∼30000× improvement described herein reflects a combination of several factors. These factors include a doubling in the label surface concentration on each particle, a larger Raman scattering cross section for the new label (∼5×), and the use of monoclonal vs polyclonal antibodies (∼1000× higher affinity). We have also employed a different Raman spectrometer. This instrument uses a slightly shorter excitation wavelength for greater enhancement and has an improved collection efficiency (>4× improvement in signal-to-noise ratio). While only rough estimates, it is the combination of these improvements that lead to the markedly lower limit of detection in our PSA assay.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.