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Volumn 70, Issue 7, 1998, Pages 1233-1240

Microminiaturized Immunoassays Using Atomic Force Microscopy and Compositionally Patterned Antigen Arrays

Author keywords

[No Author keywords available]

Indexed keywords

11,11' DITHIO BIS(SUCCINIMIDYLUNDECANOATE); 11,11'-DITHIO-BIS(SUCCINIMIDYLUNDECANOATE); DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; GOLD; IMMUNOGLOBULIN G; N OCTADECYL MERCAPTAN; N-OCTADECYL MERCAPTAN; POLYSORBATE; SUCCINIMIDE DERIVATIVE; THIOL DERIVATIVE;

EID: 0032040253     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac971125y     Document Type: Article
Times cited : (129)

References (67)
  • 1
    • 0010685337 scopus 로고    scopus 로고
    • Immunochemical Technology for Environmental Applications
    • American Chemical Society: Washington, DC
    • Aga, D. S.; Thurman, E. M. Immunochemical Technology for Environmental Applications; ACS Symposium Series 657; American Chemical Society: Washington, DC, 1996.
    • (1996) ACS Symposium Series 657
    • Aga, D.S.1    Thurman, E.M.2
  • 38
    • 1542638303 scopus 로고    scopus 로고
    • note
    • -1 following UV irradiation. This band, in the S-O stretching region, is consistent with the presence of oxygenated forms of sulfur.
  • 46
    • 1542533332 scopus 로고    scopus 로고
    • note
    • 39,40 Such a situation may compound the difference in the structural ordering of the two different adlayers and further heighten the difference in compressibility. Third, the attractive interactions between neighboring succinimidyl groups in the DSU adlayer are greater than those between the neighboring methyl groups of the ODT adlayer. In this case, the ODT adlayer may be more compressible than the DSU adlayer. The extent of the contributions of each of these interactions is, however, unknown.
  • 53
    • 85088076694 scopus 로고    scopus 로고
    • note
    • 47
  • 61
    • 1542428559 scopus 로고    scopus 로고
    • note
    • We also have evidence from characterizations using IRRAS which, though less defined, supports the covalent immobilization of IgG to DSU. The presence of new amide stretches that would arise from the covalent attachment of IgG to DSU is overwhelmed by the amide bands present in the native antibody. However, we have seen a clear decrease in the carbonyl stretches associated with the succinimidyl moiety, which in turn indicates the cleavage of the succinimidyl ring from the chain. This observation, in turn, suggests that a covalent linkage is formed between the antibody and DSU since we do not detect any hydrolysis of the end group upon exposure to solutions devoid of antibody.
  • 63
    • 85088076029 scopus 로고    scopus 로고
    • note
    • N ∼40 nN). Under these scanning forces, the bound protein regions remain viable. This observation further supports the concept that the antibodies are covalently linked to the surface.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.