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2 refers to the metal nanoparticle to which Cc has been previously conjugated. For example, Ag:Cc:Au refers to Cc:Au conjugates adsorbed at the surface of aggregated Ag nanoparticles. Ag: Cc refers to Cc added directly to aggregated colloidal Ag, without first adsorbing it to Au particles.
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A slight shift in the position of the absorbance maximum is expected upon protein binding, due to the change in the dielectric at the surface of the particle (For example, see: Underwood, S.; Mulvaney, P. Langmuir 1994, 10, 3427-3430.).
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Stabilizers were observed in TEM images of the particles (data not shown)
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Stabilizers were observed in TEM images of the particles (data not shown).
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No vibrations attributable to heme are observed for PEG-stabilized Cc:Au at 647.1- or 488.0-nm excitation (data not shown). For 568.2 nm excitation, however, weak heme vibrations are observable in the PEG-stabilized Cc: Au SERS at aggregated Ag (Supporting Information).
-
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note
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Actually, type A samples are expected to contain ∼10% more Cc than type B, since the excess Cc in Cc:Au conjugates is expected to have been removed in the centrifugation/resuspension step.
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93
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note
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Ag:Cc and Ag:Cc:Au samples were freshly prepared and run one immediately after the other, with no change in beam steering or collection optics, so that they could be directly compared.
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94
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11744255101
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note
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ex = 568.2 nm spectra may arise from vibrations not observable with standard excitations, either because of its symmetry (e.g., nontotally symmetric), or its origin (e.g., perhaps it is a protein-based rather than a heme-based mode).
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95
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11744344487
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note
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All bulk SERS measurements, because they interrogate many molecules, report only on the average orientation of molecules. A notable exception in the SERS literature is recent work by Nie and Emory showing single molecule SERS for rhodamine 6G adsorbed to isolated Ag particles.55
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Pemberton, J.E.1
Bryant, M.A.2
Sobocinski, R.L.3
Joa, S.L.4
-
100
-
-
0037658386
-
-
(b) Gao, X.; Davies, J. P.; Weaver, M. J. J. Phys. Chem. 1990, 94, 6858-6864.
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-
Gao, X.1
Davies, J.P.2
Weaver, M.J.3
-
103
-
-
11744383581
-
-
note
-
4, mode has both in-plane and out-of-plane components.
-
-
-
-
105
-
-
0001098625
-
-
(a) Kitagawa, T.; Abe, M.; Ogoshi, H. J. Chem. Phys. 1978, 69, 4516-4525.
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Kitagawa, T.1
Abe, M.2
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106
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0001098626
-
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(b) Abe, M.; Kitagawa, T.; Kyogoku, Y. J. Chem. Phys. 1978, 69, 4526-4534.
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-
Abe, M.1
Kitagawa, T.2
Kyogoku, Y.3
-
107
-
-
11744282428
-
-
note
-
1g modes.
-
-
-
-
108
-
-
0000998834
-
-
(a) Shelnutt, J. A.; Cheung, L. D.; Chang, R. C. C.; Yu, N.-T.; Felton, R. H. J. Chem. Phys. 1977, 66, 3387-3398.
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Shelnutt, J.A.1
Cheung, L.D.2
Chang, R.C.C.3
Yu, N.-T.4
Felton, R.H.5
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109
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-
36749105815
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-
(b) Shelnutt, J. A.; O'Shea, D. C; Yu, N.-T.; Cheung, L. D.; Felton, R. H. J. Chem. Phys. 1976, 64, 1156-1165.
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-
Shelnutt, J.A.1
O'Shea, D.C.2
Yu, N.-T.3
Cheung, L.D.4
Felton, R.H.5
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110
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-
1842365512
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(a) Wood, L. L.; Cheng, S.-S.; Edminston, P. L.; Saavedra, S. S. J. Am. Chem. Soc. 1997, 119, 571-576.
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Wood, L.L.1
Cheng, S.-S.2
Edminston, P.L.3
Saavedra, S.S.4
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111
-
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0029804670
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(b) Firestone, M. A.; Shank. M. L.; Sligar, S. G.; Bohn, P. W. J. Am. Chem. Soc. 1996, 118, 9033-9041.
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Firestone, M.A.1
Shank, M.L.2
Sligar, S.G.3
Bohn, P.W.4
-
112
-
-
0001514837
-
-
(c) Jiang, M.; Nolting, B.; Stayton, P. S.; Sligar, S. G. Langmuir 1996, 12, 1278-1283.
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Jiang, M.1
Nolting, B.2
Stayton, P.S.3
Sligar, S.G.4
-
113
-
-
11744261771
-
-
note
-
ex = 514.5 nm).
-
-
-
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