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Volumn 102, Issue 47, 1998, Pages 9404-9413

Protein: Colloid conjugates for surface enhanced raman scattering: Stability and control of protein orientation

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Indexed keywords


EID: 4243998148     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp982723z     Document Type: Article
Times cited : (231)

References (113)
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    • note
    • 2 refers to the metal nanoparticle to which Cc has been previously conjugated. For example, Ag:Cc:Au refers to Cc:Au conjugates adsorbed at the surface of aggregated Ag nanoparticles. Ag: Cc refers to Cc added directly to aggregated colloidal Ag, without first adsorbing it to Au particles.
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    • note
    • 46
  • 87
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    • A slight shift in the position of the absorbance maximum is expected upon protein binding, due to the change in the dielectric at the surface of the particle (For example, see: Underwood, S.; Mulvaney, P. Langmuir 1994, 10, 3427-3430.).
    • (1994) Langmuir , vol.10 , pp. 3427-3430
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    • Stabilizers were observed in TEM images of the particles (data not shown)
    • Stabilizers were observed in TEM images of the particles (data not shown).
  • 91
    • 11744290830 scopus 로고    scopus 로고
    • note
    • No vibrations attributable to heme are observed for PEG-stabilized Cc:Au at 647.1- or 488.0-nm excitation (data not shown). For 568.2 nm excitation, however, weak heme vibrations are observable in the PEG-stabilized Cc: Au SERS at aggregated Ag (Supporting Information).
  • 92
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    • note
    • Actually, type A samples are expected to contain ∼10% more Cc than type B, since the excess Cc in Cc:Au conjugates is expected to have been removed in the centrifugation/resuspension step.
  • 93
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    • note
    • Ag:Cc and Ag:Cc:Au samples were freshly prepared and run one immediately after the other, with no change in beam steering or collection optics, so that they could be directly compared.
  • 94
    • 11744255101 scopus 로고    scopus 로고
    • note
    • ex = 568.2 nm spectra may arise from vibrations not observable with standard excitations, either because of its symmetry (e.g., nontotally symmetric), or its origin (e.g., perhaps it is a protein-based rather than a heme-based mode).
  • 95
    • 11744344487 scopus 로고    scopus 로고
    • note
    • All bulk SERS measurements, because they interrogate many molecules, report only on the average orientation of molecules. A notable exception in the SERS literature is recent work by Nie and Emory showing single molecule SERS for rhodamine 6G adsorbed to isolated Ag particles.55
  • 97
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    • Clark, R. J., Hester, R. P., Eds.; Wiley: New York
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    • note
    • 4, mode has both in-plane and out-of-plane components.
  • 107
    • 11744282428 scopus 로고    scopus 로고
    • note
    • 1g modes.
  • 113
    • 11744261771 scopus 로고    scopus 로고
    • note
    • ex = 514.5 nm).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.