Noninvasive Imaging of Transgene Expression by Use of Positron Emission Tomography in a Pig Model of Myocardial Gene Transfer

Circulation

Volumn 108, Issue 17, 2003, Pages 2127-2133

  Bengel, Frank M ^b   Anton, Martina ^c   Richter, Thomas ^a   Simoes, Marcus V ^c   Haubner, Roland ^c   Henke, Julia ^c   Erhardt, Wolf ^c   Reder, Sybille ^c   Lehner, Terry ^c   Brandau, Wolfgang ^c   Boekstegers, Peter ^c   Nekolla, Stephan G ^c   Gansbacher, Bernd ^c   Schwaiger, Markus ^c  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c NONE

Author keywords

Gene therapy; Genes; Imaging; Radioisotopes

Indexed keywords

2' FLUORO 2' DEOXY 5 IODO 1 BETA DEXTRO ARABINOFURANOSYLURACIL I 124; ADENOVIRUS VECTOR; AMMONIA; GENE PRODUCT; NITROGEN 13; RADIOISOTOPE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; COMPARTMENT MODEL; CONTROLLED STUDY; DIAGNOSTIC IMAGING; EUTHANASIA; GENE; GENE EXPRESSION; GENE TRANSFER; HEART MUSCLE; HEART MUSCLE BLOOD FLOW; HERPESVIRAL THYMIDINE KINASE REPORTER GENE; IMMUNOHISTOCHEMISTRY; INFLAMMATION; INTRAMYOCARDIAL VIRUS INJECTION; NON INVASIVE MEASUREMENT; NONHUMAN; NUCLEOSIDE METABOLISM; POSITRON EMISSION TOMOGRAPHY; PRIORITY JOURNAL; PROTEIN METABOLISM; RADIOACTIVITY; REPORTER GENE; SWINE; TRANSGENE; VIRAL GENE DELIVERY SYSTEM; VIRUS INFECTION;

ANIMALS; ARABINOFURANOSYLURACIL; BLOOD FLOW VELOCITY; CORONARY CIRCULATION; FEASIBILITY STUDIES; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; HEART; IODINE RADIOISOTOPES; MODELS, ANIMAL; MYOCARDIUM; SWINE; THYMIDINE KINASE; TOMOGRAPHY, EMISSION-COMPUTED; TRANSGENES;

EID: 0242349749     PISSN: 00097322     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.CIR.0000091401.26280.A0     Document Type: Article

References (24)

1. Isner JM. Myocardial gene therapy. Nature. 2002;415:234-239.
2. Losordo DW, Vale PR, Hendel RC, et al. Phase 1/2 placebo-controlled, double-blind, dose-escalating trial of myocardial vascular endothelial growth factor 2 gene transfer by catheter delivery in patients with chronic myocardial ischemia. Circulation. 2002;105:2012-2018.
3. Wu JC, Inubushi M, Sundaresan G, et al. Optical imaging of cardiac reporter gene expression in living rats. Circulation. 2002;105:1631-1634.
4. Wu JC, Inubushi M, Sundaresan G, et al. Positron emission tomography imaging of cardiac reporter gene expression in living rats. Circulation. 2002;106:180-183.
5. Inubushi M, Wu JC, Gambhir SS, et al. Positron-emission tomography reporter gene expression imaging in rat myocardium. Circulation. 2003;107:326-333.
6. Brust P, Haubner R, Friedrich A, et al. Comparison of [18F]FHPG and [124/125I]FIAU for imaging herpes simplex virus type 1 thymidine kinase gene expression. Eur J Nucl Med. 2001;28:721-729.
7. Nekolla SG, Miethaner C, Nguyen N, et al. Reproducibility of polar map generation and assessment of defect severity and extent assessment in myocardial perfusion imaging using positron emission tomography. Eur J Nucl Med. 1998;25:1313-1321.
8. Hutchins GD, Schwaiger M, Rosenspire KC, et al. Noninvasive quantification of regional blood flow in the human heart using N-13 ammonia and dynamic positron emission tomographic imaging. J Am Coll Cardiol. 1990;15:1032-1042.
9. Hackett NR, El Sawy T, Lee LY, et al. Use of quantitative TaqMan real-time PCR to track the time-dependent distribution of gene transfer vectors in vivo. Mol Ther. 2000;2:649-656.
10. Haberkorn U, Altmann A, Morr I, et al. Monitoring gene therapy with herpes simplex virus thymidine kinase in hepatoma cells: uptake of specific substrates. J Nucl Med. 1997;38:287-294.
11. Haubner R, Avril N, Hantzopoulos PA, et al. In vivo imaging of herpes simplex virus type 1 thymidine kinase gene expression: early kinetics of radiolabelled FIAU. Eur J Nucl Med. 2000;27:283-291.
12. Gambhir SS, Barrio JR, Wu L, et al. Imaging of adenoviral-directed herpes simplex virus type 1 thymidine kinase reporter gene expression in mice with radiolabeled ganciclovir. J Nucl Med. 1998;39:2003-2011.
13. Tjuvajev JG, Stockhammer G, Desai R, et al. Imaging the expression of transfected genes in vivo. Cancer Res. 1995;55:6126-6132.
14. Jacobs A, Voges J, Reszka R, et al. Positron-emission tomography of vector-mediated gene expression in gene therapy for gliomas. Lancet. 2001;358:727-729.
15. Bengel FM, Anton M, Avril N, et al. Uptake of radiolabeled 22′-fluoro-2′-deoxy-5-iodo-1-beta-D-arabinofuranosyluracil in cardiac cells after adenoviral transfer of the herpesvirus thymidine kinase gene: the cellular basis for cardiac gene imaging. Circulation. 2000;102:948-950.
16. Tjuvajev JG, Doubrovin M, Akhurst T, et al. Comparison of radiolabeled nucleoside probes (FIAU, FHBG, and FHPG) for PET imaging of HSV1-tk gene expression. J Nucl Med. 2002;43:1072-1083.
17. Muhlhauser J, Jones M, Yamada I, et al. Safety and efficacy of in vivo gene transfer into the porcine heart with replication-deficient, recombinant adenovirus vectors. Gene Ther. 1996;3:145-153.
18. Tjuvajev JG, Chen SH, Joshi A, et al. Imaging adenoviral-mediated herpes virus thymidine kinase gene transfer and expression in vivo. Cancer Res. 1999;59:5186-5193.
19. Hunsucker SA, Spychala J, Mitchell BS. Human cytosolic 5′-nucleotidase I: characterization and role in nucleoside analog resistance. J Biol Chem. 2001;276:10498-10504.
20. Rampazzo C, Gallinaro L, Milanesi E, et al. A deoxyribonucleotidase in mitochondria: involvement in regulation of dNTP pools and possible link to genetic disease. Proc Natl Acad Sci U S A. 2000;97:8239-8244.
21. McKenzie R, Fried MW, Sallie R, et al. Hepatic failure and lactic acidosis due to fialuridine (FIAU), an investigational nucleoside analogue for chronic hepatitis B. N Engl J Med. 1995;333:1099-1105.
22. Avril N, Bengel FM. Defining the success of cardiac gene therapy: how can nuclear imaging contribute? Eur J Nucl Med Mol Imaging. 2003;30:757-771.
23. Iyer M, Wu L, Carey M, et al. Two-step transcriptional amplification as a method for imaging reporter gene expression using weak promoters. Proc Natl Acad Sci U S A. 2001;98:14595-14600.
24. Doubrovin M, Ponomarev V, Beresten T, et al. Imaging transcriptional regulation of p53-dependent genes with positron emission tomography in vivo. Proc Natl Acad Sci U S A. 2001;98:9300-9305.