Endoreplication and polyploidy in primary culture of rat hepatic stellate cells

Cell and Tissue Research

Volumn 313, Issue 3, 2003, Pages 301-311

  Dudas, Jozsef ^a   Saile, Bernhard ^a   El Armouche, Hamudi ^a   Aprigliano, Isabella ^a   Ramadori, Giuliano ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Cyclin D1; Endoreplication; HSC; Myofibroblast; pRb; Rat (Wistar)

Indexed keywords

CELL DNA; CYCLIN D1; CYCLIN E1; CYCLINE; RETINOBLASTOMA PROTEIN; SCLEROPROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL LEVEL; CELL PROLIFERATION; CELL VOLUME; CONTROLLED STUDY; DNA CONTENT; DNA SYNTHESIS; ENDOREDUPLICATION; LIVER CELL; MITOSIS; MULTINUCLEAR CELL; MYOFIBROBLAST; NONHUMAN; POLYPLOIDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STELLATE CELL;

ANIMALS; APOPTOSIS; BLOTTING, NORTHERN; CELL COUNT; CELL DIVISION; CELL SIZE; CELLS, CULTURED; CYCLIN D1; DNA; HEPATOCYTES; IMMUNOHISTOCHEMISTRY; POLYPLOIDY; RATS; RATS, WISTAR; RNA, MESSENGER; THYMIDINE; TIME FACTORS; TRITIUM;

ANIMALIA;

EID: 0141887403     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00441-003-0768-3     Document Type: Article

References (47)

1. Bissell DM (1992) Lipocyte activation and hepatic fibrosis. Gastroenterology 102:1803-1805
2. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299
3. Crowe WE, Altamirano J, Huerto L, Alvarez-Leefmans FJ (1995) Volume changes in single N1E-115 neuroblastoma cells measured with a fluorescent probe. Neuroscience 69:283-296
4. Darzynkiewicz Z, Williamson B, Carswell EA, Old LJ (1984) Cell cycle-specific effects of tumor necrosis factor. Cancer Res 44:83-90
5. de Leeuw AM, Brouwer A, Barelds RJ, Knook DL (1983) Maintenance cultures of Kupffer cells isolated from rats of various ages: ultrastructure, enzyme cytochemistry, and endocytosis. Hepatology 3:497-506
6. de Leeuw AM, McCarthy SP, Geerts A, Knook DL (1984) Purified rat liver fat-storing cells in culture divide and contain collagen. Hepatology 4:392-403
7. Dignam JD, Lebovitz RM, Roeder RG (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11:1475-1489
8. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Liskens M, Rublelj I, Pereira-Smith O, Peacocke M (1995) A biomarker that identifies senescent human cells in culture and aging skin in vivo. Proc Natl Acad Sci U S A 92:9363-9367
9. Edgar BA, Orr-Weaver TL (2001) Endoreplication cell cycles: more for less. Cell 105:297-306
10. Fehrenbach H, Weiskirchen R, Kasper M, Gressner AM (2001) Up-regulated expression of the receptor for advanced glycation end products in cultured rat hepatic stellate cells during transdifferentiation to myofibroblasts. Hepatology 34:943-952
11. Gandarillas A, Davies D, Blanchard JM (2000) Normal and c-Myc-promoted human keratinocyte differentiation both occur via a novel cell cycle involving cellular growth and endoreplication. Oncogene 19:3278-3289
12. Garcia P, Cales C (1996) Endoreplication in megakaryoblastic cell lines is accompanied by sustained expression of G1/S cyclins and downregulation of cdc25C. Oncogene 15:695-703
13. Gupta S (2000) Hepatic polyploidy and liver growth control. Semin Cancer Biol 10:161-171
14. Hanselmann RG, Oberringer M (2001) Polyploidization: a Janus-faced mechanism. Med Hypotheses 56:58-64
15. Hautekeete ML, Geerts A (1997) The hepatic stellate (Ito) cell: its role in human liver disease. Virchows Arch 430:195-207
16. Iredale JP (2001) Hepatic stellate cell behavior during resolution of liver injury. Semin Liver Dis 21:427-436
17. Jack EM, Bentley P, Bieri F, Muakkassah-Kelly SF, Staubli W, Suter J, Waechter F, Cruz-Orive LM (1990) Increase in hepatocyte and nuclear volume and decrease in the population of binucleated cells in preneoplastic foci of rat liver: a stereological study using the nucleator method. Hepatology 11:286-297
18. Jiang Z, Liang P, Leng R, Guo Z, Liu Y, Liu X, Bubnic S, Keating A, Murray D, Goss P Zacksenhaus E (2000) E2F1 and p53 are dispensable, whereas p21(Waf1/Cip1) cooperates with Rb to restrict endoreduplication and apoptosis during skeletal myogenesis. Dev Biol 227:8-41
19. Knittel T, Dinter C, Kobold D, Neubauer K, Mehde M, Eichhorst S, Ramadori G (1999a) Expression and regulation of cell adhesion molecules by hepatic stellate cells (HSC) of rat liver: involvement of HSC in recruitment of inflammatory cells during hepatic tissue repair. Am J Pathol 154:153-167
20. Knittel T, Kobold D, Piscaglia F, Saile B, Neubauer K, Mehde M, Timpl R, Ramadori G (1999b) Localization of liver myofibroblasts and hepatic stellate cells in normal and diseased rat livers: distinct roles of (myo-)fibroblast subpopulations in hepatic tissue repair. Histochem Cell Biol 112:387-401
21. Knittel T, Kobold D, Saile B, Grundmann A, Neubauer K, Piscaglia F, Ramadori G (1999c) Rat myofibroblasts and hepatic stellate cells: different cell populations of the fibroblast lineage with fibrogenic potential. Gastroenterology 117:1205-1222
22. Knook DL, Blansjaar N, Sleyster EC (1977) Isolation and characterization of Kupffer and endothelial cells from the rat liver. Exp Cell Res 109:317-329
23. Kobold D, Grundmann A, Piscaglia F, Eisenbach C, Neubauer K, Steffgen J, Ramadori G, Knittel T (2002) Expression of reelin in hepatic stellate cells and during hepatic tissue repair: a novel marker for the differentiation of HSC from other liver myofibroblasts. J Hepatol 36:607-613
24. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
25. Larkins BA, Dilkes BP, Dante RA, Coelho CM, Woo YM, Liu Y (2001) Investigating the hows and whys of DNA endoreduplication. J Exp Bot 52:183-192
26. Li X, Darzynkiewicz Z (1995) Labeling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif 28:571-579
27. Mak KM, Lieber CS (1988) Lipocytes and transitional cells in alcoholic liver disease: a morphometric study. Hepatology 8:1027-1033
28. Mendoza-Figueroa T, Arguello C, Kuri-Harcuch W (1988) Culture of proliferating and differentiating fat-storing cells in 3T3-conditioned medium. Biol Cell 64:29-38
29. Murakami K, Abe T, Miyazawa M, Yamaguchi M, Masuda T, Matsuura T, Nagamori S, Takeuchi K, Abe K, Kyogoku M (1995) Establishment of a new human cell line LI90, exhibiting characteristics of hepatic Ito (fat-storing) cells. Lab Invest 72:731-739
30. Nakano M, Lieber CS (1982) Ultrastructure of initial stages of perivenular fibrosis in alcohol-fed baboons. Am J Pathol 106:145-155
31. Neubauer K, Knittel T, Aurisch S, Fellmer P, Ramadori G (1996) Glial fibrillary acidic protein - a cell type specific marker for Ito cells in vivo and in vitro. J Hepatol 24:719-730
32. Niculescu AB 3rd, Chen X, Smeets M, Hengst L, Prives C, Reed SI (1998) Effects of p21(Cip1/Waf1) at both the G1/S and the G2/ M cell cycle transitions: pRb is a critical determinant in blocking DNA replication and in preventing endoreduplication. Mol Cell Biol 18:629-643
33. Obaya AJ, Sedivy JM (2002) Regulation of cyclin-Cdk activity in mammalian cells. Cell Mol Life Sci 59:126-142
34. Paunesku T, Mittal S, Protic M, Oryhon J, Korolev SV, Joachimiak A, Woloschak GE (2001) Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol 77:1007-1021
35. Ramadori G, Saile B (2002) Mesenchymal cells in the liver - one cell type or two? Liver 22:283-294
36. Ramadori G, Veit T, Schwogler S, Dienes HP, Knittel T, Rieder H, Meyer zum Büschenfelde KH (1990) Expression of the gene of the alpha-smooth muscle-actin isoform in rat liver and in rat fat-storing (ITO) cells. Virchows Arch B Cell Pathol Mol Pathol 59:349-357
37. Saile B, Knittel T, Matthes N, Ramadori G (1997) CD95/CD95L mediated apoptosis of the hepatic stellate cell. A mechanism terminating uncontrolled HSC proliferation during hepatic tissue repair. Am J Pathol 151:1265-1272
38. Saile B, Matthes N, Knittel T, Ramadori G (1999) Transforming growth factor beta and tumor necrosis factor alpha inhibit both apoptosis and proliferation of activated rat hepatic stellate cells. Hepatology 30:196-202
39. Saile B, Matthes N, El Armouche H, Neubauer K, Ramadori G (2001) The be1, NFkappaB and p53/p21WAF1 systems are involved in spontaneous apoptosis and in the anti-apoptotic effect of TGF-beta or TNF-alpha on activated hepatic stellate cells. Eur J Cell Biol 80:554-561
40. Saile B, Matthes N, Neubauer K, Eisenbach C, El-Armouche H, Ramadori G (2002) Rat liver myofibroblasts (rMF) and "activated" hepatic stellate cells (HSC) differ in gene expression of components of the CD95-system as well as in response on TGF-beta stimulation. Am J Physiol Gastrointest Liver Physiol 283:0435-444
41. Sasaki T, Wiedemann H, Matzner M, Chu ML, Timpl R (1996) Expression of fibulin-2 by fibroblasts and deposition with fibronectin into a fibrillar matrix. J Cell Sci 109:2895-2904
42. Schnabl B, Purbeck CA, Choi YH, Hagedorn CH, Brenner DA (2003) Replicative senescence of activated human hepatic stellate cells is accompanied by a pronounced inflammatory but less fibrogenic phenotype. Hepatology 37:653-664
43. Scott FW, Forsdyke DR (1978) The rate of deoxyribonucleic acid synthesis by cultured Chinese-hamster ovary cells. An application of isotope-dilution analysis. Biochem J 170:545-549
44. Senoo H, Hata R (1994) Extracellular matrix regulates and L-ascorbic acid 2-phosphate further modulates morphology, proliferation, and collagen synthesis of perisinusoidal stellate cells. Biochem Biophys Res Commun 200:999-1006
45. I moderately increases ploidy in megakaryocytes. Haematologica 86:17-23
46. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76:4350-4354
47. Wagner M, Hampel B, Bernhard D, Hala M, Zwerschke W, Jansen-Durr P (2001) Replicative senescence of human endothelial cells in vitro involves Gl arrest, polyploidization and senescence-associated apoptosis. Exp Gerontol 36:1327-1347