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Volumn 13, Issue 20, 2003, Pages 3587-3592

Adenosine-anchored triphosphate subsite probing: Distinguishing between HER-2 and HER-4 tyrosine protein kinases

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE; EPIDERMAL GROWTH FACTOR RECEPTOR; HETEROCYCLIC COMPOUND; PHOSPHATE; PROTEIN TYROSINE KINASE; TRIPHOSPHATE; UNCLASSIFIED DRUG;

EID: 0141516623     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(03)00761-3     Document Type: Article
Times cited : (4)

References (48)
  • 22
    • 0141599428 scopus 로고    scopus 로고
    • The X-ray structure of EGFR kinase domain has recently become available
    • The X-ray structure of EGFR kinase domain has recently become available Stamos J., Silwkowski M.X., Eigenbrot C. J. Biol. Chem. 48:2002;46265.
    • (2002) J. Biol. Chem. , vol.48 , pp. 46265
    • Stamos, J.1    Silwkowski, M.X.2    Eigenbrot, C.3
  • 33
    • 85031084544 scopus 로고    scopus 로고
    • The accession codes for proteins analysed are: P04626(HER-2); Q15303(HER-4); 1ATP_E(PKA); and 1BYG_A (Src).
    • Sequence analyses were performed using the Blast program ( http://www.ncbi.nlm.nih.gov ). The accession codes for proteins analysed are: P04626(HER-2); Q15303(HER-4); 1ATP_E(PKA); and 1BYG_A (Src).
  • 45
    • 85031067515 scopus 로고    scopus 로고
    • 20 -42.9° (c 0.20, MeOH).
  • 46
    • 0034663983 scopus 로고    scopus 로고
    • note
    • m is 5 μM, F is the ratio between the initial rates of phosphorylated peptide formation without or with the presence of the inhibitor, and [S] is the ATP concentration. See: Jan A.Y., Johnson E.F., Diamonti J.A., Carraway K.L. III, Anderson K.S. Biochemistry. 39:2000;9786.
    • (2000) Biochemistry , vol.39 , pp. 9786
    • Jan, A.Y.1    Johnson, E.F.2    Diamonti, J.A.3    Carraway K.L. III4    Anderson, K.S.5
  • 47
    • 85031085373 scopus 로고    scopus 로고
    • HER-2 and phosphorylated HER-2 have different kinase activities. Autophosphorylation of HER-2 results in heterogeneity in enzyme population during the course of assay. The prephosphorylation of HER-2 was performed in excess of ATP for 30 min on ice and quenched with 0.5 M EDTA. The phosphorylated HER-2 was purified by using Bio-Spin™ columns.
  • 48
    • 85031075547 scopus 로고    scopus 로고
    • The complex kinetic characteristics may arise from the small population of HER-2 still remaining from the prephosphorylation reaction of HER-2. See ref 18a.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.