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85031084544
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The accession codes for proteins analysed are: P04626(HER-2); Q15303(HER-4); 1ATP_E(PKA); and 1BYG_A (Src).
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Sequence analyses were performed using the Blast program ( http://www.ncbi.nlm.nih.gov ). The accession codes for proteins analysed are: P04626(HER-2); Q15303(HER-4); 1ATP_E(PKA); and 1BYG_A (Src).
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36
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0033596738
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45
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85031067515
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20 -42.9° (c 0.20, MeOH).
-
-
-
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46
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0034663983
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note
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m is 5 μM, F is the ratio between the initial rates of phosphorylated peptide formation without or with the presence of the inhibitor, and [S] is the ATP concentration. See: Jan A.Y., Johnson E.F., Diamonti J.A., Carraway K.L. III, Anderson K.S. Biochemistry. 39:2000;9786.
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Jan, A.Y.1
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Carraway K.L. III4
Anderson, K.S.5
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47
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-
85031085373
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HER-2 and phosphorylated HER-2 have different kinase activities. Autophosphorylation of HER-2 results in heterogeneity in enzyme population during the course of assay. The prephosphorylation of HER-2 was performed in excess of ATP for 30 min on ice and quenched with 0.5 M EDTA. The phosphorylated HER-2 was purified by using Bio-Spin™ columns.
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-
-
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48
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85031075547
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The complex kinetic characteristics may arise from the small population of HER-2 still remaining from the prephosphorylation reaction of HER-2. See ref 18a.
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