Optical single transporter recording: Transport kinetics in microarrays of membrane patches

Annual Review of Biophysics and Biomolecular Structure

Volumn 32, Issue , 2003, Pages 47-67

  Peters, Reiner ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Confocal microscopy; Membrane transport kinetics; Membrane transporters; Nuclear transport; Photobleaching

Indexed keywords

ABC TRANSPORTER; CARRIER PROTEIN;

ANALYTIC METHOD; BLEACHING; CONFOCAL MICROSCOPY; ENDOPLASMIC RETICULUM; FLUORESCENCE MICROSCOPY; MATHEMATICAL ANALYSIS; MEMBRANE TRANSPORT; NONHUMAN; NUCLEAR PORE; NUCLEOCYTOPLASMIC TRANSPORT; OPTICAL SINGLE TRANSPORTER RECORDING; PATCH CLAMP; PHOTOACTIVATION; PRIORITY JOURNAL; RECORDING; REVIEW; TRANSPORT KINETICS;

ACTIVE TRANSPORT, CELL NUCLEUS; CELL MEMBRANE; CELL NUCLEUS; DIFFUSION; EQUIPMENT DESIGN; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MOTION; NANOTECHNOLOGY; NUCLEAR ENVELOPE; NUCLEAR PORE; NUCLEAR PROTEINS; OPTICS; PATCH-CLAMP TECHNIQUES; PHOTOBLEACHING; PHOTOLYSIS;

EID: 0042855028     PISSN: 10568700     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.biophys.32.110601.142429     Document Type: Review

References (99)

1. Adam SA, Stern-Marr R, Gerace L. 1990. Nuclear protein import in permeablized mammalian cells requires soluble cytoplasmic factors. J. Cell Biol. 111:807-16
2. Agarrabes FA, Dice JF. 2001. Protein translocation across membranes. Biochim. Biophys. Acta 1513:1-24
3. André B. 1995. An overview of membrane transport proteins in Saccharomyces cerevisiae. Yeast 11:1575-611
4. Axelrod D, Koppel DE, Schlessinger J, Elson E, Webb WW. 1976. Mobility measurement by analysis of fluorescence photobleaching recovery. Biophys. J. 16:1055-69
5. Baker PF, Willis JS. 1962. Binding of the cardiac glycoside ouabain to intact cell. J. Physiol. 224:441-62
6. Basché T, Nie S, Fernandez JM. 2001. Single molecules. Proc. Natl. Acad. Sci. USA 98:10527-28
7. Bayliss B, Corbett AH, Stewart M. 2000. The molecular mechanism of transport of macromolecules through nuclear pore complexes. Traffic 1:448-56
8. Bill RM. 2001. Yeats - a panacea for the structure-function analysis of membrane proteins? Curr. Genet. 40:157-71
9. Bischoff FR, Ponstingl H. 1995. Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1. Methods Enzymol. 257:135-44
10. Carslaw FS, Jaeger JC. 1959. Conduction of Heat in Solids, pp. 99-102. Oxford: Clarendon. 2nd ed.
11. Cha A, Zerangue N, Kavanaugh M, Bezanilla F. 1998. Fluorescence techniques for studying cloned channels and transporters expressed in Xenopus oocytes. Methods Enzymol. 296:566-78
12. Chen Y, Pépin A. 2001. Nanofabrication: conventional and nonconventional methods. Electrophoresis 22:187-207
13. Crook YM, Blobel G. 2001. Karyopherins and nuclear transport. Curr. Opin. Struct. Biol. 11:703-15
14. Dean M, Hamin Y, Chimini G. 2001. The human ATP-binding cassette (ABC) transporter superfamily. J. Lipid Res. 42:1007-17
15. Dietrich TR, Ehrfeld W, Lacher M, Krämer M, Speot B. 1996. Fabrication technologies for microsystems utilizing photoetchable glass. Microelectron. Eng. 30:497-504
16. Edidin M, Zagyansky Y, Lardner TJ. 1976. Measurement of membrane protein lateral diffusion in single cells. Science 191:466-68
17. Deleted in proof
18. Englmeier L, Fornerod M, Bishoff FR, Petosa C, Mattaj IW, Kutay U. 2001. RanBP3 influences interactions between CRM1 and its nuclear protein export substrates. EMBO Rep. 2:926-32
19. Englmeier L, Olivo JC, Mattaj IW. 1999. Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis. Curr. Biol. 9:1-41
20. Fertig N, Meyer C, Blick RH, Trautmann C, Behrends JC. 2001. Microstructured glass chips for ion-channel electrophysiology. Phys. Rev. E 64, 040901-1-4
21. Focht DC, Spector DL, Goldman RD, Leinwand LA. 1998. Observation of live cells on the light microscope. In Cells: A Laboratory Manual, ed. DL Spector, RD Goldman, LA Leinwand, pp. 75:1-13. New York: Cold Spring Harbor Press
22. Görlich D, Pante N, Kutay U, Aebi U, Bischoff FR. 1996. Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15:5584-94
23. ++ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260:3440-50
24. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfluegers Arch. 391:85-100
25. Hassel I, Cezanne V, Trevino C, Schlatter H, Romero-Matuschek I, et al. 1966. Export of ribosomal subunits from resealed rat liver nuclear envelopes. Eur. J. Biochem. 241:32-37
26. Haupts U, Tittor J, Oesterhelt D. 1999. Closing in on bacteriorhodopsin: progress in understanding the molecule. Annu. Rev. Biophys. Biomol. Struct. 28:367-99
27. Hill K, Model K, Ryan M, Dietmeier K, Martin F, et al. 1998. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature 395:516-21
28. Holland B, Blight MA. 1999. ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans. J. Mol. Biol. 293:381-99
29. Jacobson K, Wu E, Poste G. 1976. Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching. Biochim. Biophys. Acta 433:215-22
30. Jarvis SM. 2000. Assay of membrane transport in cells and membrane vesicles. In Membrane Transport, ed. SA Baldwin, pp. 1-20. Oxford: Oxford Univ. Press
31. Kahya N, Pécheur EI, de Boeji WP, Wierma DA, Hoekstra D. 2001. Reconstitution of membrane proteins into giant unilamellar vesicles via peptide-induced fusion. Biophys. J. 81:1464-74
32. Keminer O, Peters R. 1999. Permeability of single nuclear pores. Biophys. J. 77:217-28
33. Keminer O, Siebrasse JP, Zerf K, Peters R. 1999. Optical recording of signal-mediated protein transport through single nuclear pore complexes. Proc. Natl. Acad. Sci. USA 96:11842-47
34. Klein I, Sarkadi B, Varadi A. 1999. An inventory of the human ABC proteins. Biochim. Biophys. Acta 1461:237-62
35. Kose S, Imamato N, Tachibana T, Shimamoto T, Yoneda Y. 1997. Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex. J. Cell Biol. 139:841-49
36. Kubitscheck U, Heinrich O, Peters R. 1996. Continuous scanning microphotolysis: a simple laser scanning microscopic method for lateral transport measurements employing single- or two-photon excitation. Bioimaging 4:158-67
37. Kubitscheck U, Kückmann O, Kues T, Peters R. 2000. Imaging and tracking of single GFP molecules in solution. Biophys. J. 78:2170-79
38. Kubitscheck U, Kues T, Peters R. 1999. Visualization of nuclear pore complex and its distribution by confocal laser scanning microscopy. Methods Enzymol. 307:207-30
39. Deleted in proof
40. Kubitscheck U, Tschödrich-Rotter M, Wedekind P, Peters R. 1996. Two-photon scanning microphotolysis for three-dimensional data storage and biological transport measurements. J. Microsc. 182:225-33
41. Kubitscheck U, Wedekind P, Peters R. 1994. Lateral diffusion measurement at high spatial resolution by scanning microphotolysis in a confocal microscope. Biophys. J. 67:948-56
42. Kubitscheck U, Wedekind P, Zeidler O, Grote M, Peters R. 1996. Single nuclear pores visualized by confocal microscopy and image processing. Biophys. J. 70:2067-77
43. Kues T, Dickmanns A, Lührmann R, Peters R, Kubitscheck U. 2001. High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking. Proc. Natl. Acad. Sci. USA 98:12021-26
44. Kues T, Peters R, Kubitscheck U. 2001. Visualization and tracking of single protein molecules in the cell nucleus. Biophys. J. 80:2954-67
45. Laage R, Langosch D. 2001. Stragtegies for prokaryotic expression of eukaryotic membrane proteins. Traffic 2:99-104
46. Lang I, Scholz M, Peters R. 1986. Molecular mobility and nucleocytoplasmic flux in hepatoma cells. J. Cell Biol. 102:1183-90
47. Lange F de, Cambi A, Huijbens R, Bakker B de, Rensen W, et al. Cell biology beyond the diffraction limit: near-field scanning optical microscopy. J. Cell Sci. 114:4153-60
48. Deleted in proof
49. Macara IG. 2001. Transport into and out of the nucleus. Microbiol. Mol. Rev. 65:570-94
50. Maul GG. 1977. The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution. Int. Rev. Cytol. (Suppl. 6):75-186
51. Mazzanti M, Bustamante JO, Oberleithner H. 2001. Electrical dimension of the nuclear envelope. Physiol. Rev. 81:1-19
52. Nachury MV, Weis K. 1999. The direction of transport through the nuclear pore can be inverted. Proc. Natl. Acad. Sci. USA 96:9622-27
53. Nakielny S, Dreyfuss G. 1998. Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis. Curr. Biol. 8:89-95
54. Neher E, Sakmann B. 1976. Single-channel currents recorded from membrane of denervated frog fibres. Nature 260:779-802
55. Nelissen B, De Warchter R. 1997. Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccharomyces cervisiae. FEMS Microbiol. Rev. 21:113-34
56. Nemergut ME, Macara IG. 2000. Nuclear import of the Ran exchange factor, RCC1, is mediated by at least two distinct mechanisms. J. Cell Biol. 140:835-50
57. Nolte S, Momma C, Chivkov BN, Welling H. 1999. Mikrostrukturierung mit ultrakurzen Laserpulsen. Physikal. Blätter 55:41-44
58. Paine PL, Moore LC, Horowitz SB. 1975. Nuclear envelope permeability. Nature 254:109-14
59. Palmieri L, Larosa FM, Vozza A, Agrimi G, Fiermonte G, et al. 2000. Identification and functions of new transporters in yeast mitochondria. Biochim. Biophys. Acta 1459:363-69
60. Paulsen IT, Sliwinski MK, Nelissen B, Goffeau A, Saier MH. 1998. Unified inventory of established and putative transporters encoded within the complete genome of Saccharomyces cerevisiae. FEBS Lett. 430:116-25
61. Deleted in proof
62. Peters R. 1983. Nuclear envelope permeability measured by fluorescence microphotolysis of single liver cell nuclei. J. Biol. Chem. 258:11427-29
63. Peters R. 1984. Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis. EMBO J. 3:1831-36
64. Peters R. 1986. Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility. Biochim. Biophys. Acta 864:305-59
65. Peters R, Brünger A, Schulten K. 1981. Continuous fluorescence microphotolysis: a sensitive method for study of diffusion processes in single cells. Proc. Natl. Acad. Sci. USA 78:962-66
66. Peters R, Coutavas E, Siebrasse JP. 2002. Nuclear transport kinetics in microarrays of nuclear envelope patches. J. Struct. Biol. 140:268-78
67. Peters R, Kubitscheck U. 1999. Scanning microphotolysis: three-dimensional diffusion measurement and optical single-transporter recording. Methods 18:508-17
68. Peters R, Passow H. 1984. Anion transport in single erythrocyte ghosts measured by fluorescence microphotolysis. Biochim. Biophys. Acta 777:334-38
69. Peters R, Peters J, Tews KH, Bähr W. 1974. A microfluorimetric study of translational diffusion in erythrocyte membranes. Biochim. Biophys. Acta 367:282-94
70. Peters R, Saner H, Tschopp J, Fritzsch G. 1990. Transients of perforin pore formation observed by fluorescence microscopic single channel recording. EMBO J. 9:2447-51
71. Deleted in proof
72. Pun YK, Lecar H. 1998. In Cell Physiology Source Book, ed. N Sperelakis, pp. 391-405. San Diego: Academic
73. Deleted in proof
74. Radu A, Moore MS, Blobel G. 1995. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81:215-22
75. Rexach M, Blobel G. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83:683-92
76. Ribbeck K, Görlich D. 2001. Kinetic analysis of translocation through nuclear pore complexes. EMBO J. 20:1320-30
77. Ribbeck K, Kutay U, Parskava E, Görlich D. 1999. The translocation of transportin-cargo complexes through the nuclear pores is independent of both Ran and energy. Curr. Biol. 9:47-50
78. Richards SA, Carey KL, Macara IG. 1997. Requirement of guanosine-triphosphate-bound Ran for signal-mediated nuclear protein export. Science 276:1842-44
79. Rigaud JL, Pitard B, Levy D. 1995. Reconstitution of membrane proteins into liposomes: application to energy-transducing membrane proteins. Biochim. Biophys. Acta 1231:223-46
80. Rout MP, Aitchison JD, Suprapto A, Hjertaas K, Zhao Y, Chait B. 2000. The yeast nuclear pore complex: composition, architecture and transport mechanism. J. Cell Biol. 148:635-51
81. Sakmann B, Neber E. 1995. Single-Channel Recording. New York: Plenum. 2nd ed.
82. Sauer H, Pratsch L, Fritzsch G, Bhakdi S, Peters R. 1991. Complement pore genesis observed in erythrocyte membranes by fluorescence microscopic single channel recording. Biochem. J. 276:395-99
83. Sharom FJ, Liu RL, Qin Q, Romsicki Y. 2001. Exploring the structure and function of the P-glycoprotein multidrug transporter using fluorescence spectroscopic tools. Cell Dev. Biol. 12:257-66
84. Siebrasse JP, Coutavas E, Peters R. 2002. Reconstitution of nuclear protein export in isolated nuclear envelopes. J. Cell Biol. 158:849-54
85. Siebrasse JP, Peters R. 2002. Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopes. EMBO Rep. 3:887-92
86. Simon SM, Blobel G, Zimmerberg J. 1989. Large aqueous channels in membrane vesicles derived from the rough endoplasmatic reticulum of canine pancreas or the plasma membrane of Escherichia coli. Proc. Natl. Acad. Sci. USA 86:6176-80
87. Talcott B, Moore MS. 1999. Getting across the nuclear pore complex. Trends Cell Biol. 9:312-18
88. Tschödrich-Rotter M, Kubitscheck U, Ugochukwu G, Buckley Jr, Peters R. 1996. Optical single-channel analysis of the aerolysin pore in erythrocyte membranes. Biophys. J. 70:723-32
89. Tschödrich-Rotter M, Peters R. 1998. An optical method for recording the activity of single transporters in membrane patches. J. Microsc. 192:114-25
90. Tsien RY, Miyawaki A. 1998. Seeing the machinery of live cells. Science 280:1954-55
91. Van Belle D, André B. 2001. A genomic view of yeast membrane transporters. Curr. Opin. Cell Biol. 13:389-98
92. Vancurova I, Lou W, Paine TM, Paine PL. 1993. Nucleoplasmin uptake by facilitated transport and intranuclear binding. Eur. J. Cell Biol. 62:22-33
93. Vasu SK, Forbes DJ. 2001. Nuclear pores and nuclear assembly. Curr. Opin. Cell Biol. 13:363-75
94. Venosa RA, Horowicz P. 1981. Density and apparent localization of the sodium pump in frog satorius muscle. J. Physiol. 138:119-39
95. Verkman AS. 1995. Optical methods to measure membrane transport processes. J. Membr. Biol. 148:99-110
96. Verkman AS. 2000. Water permeability measurement in living cells and complex tissues. J. Membr. Biol. 173:73-87
97. Wedekind P, Kubitscheck U, Peters R. 1994. Scanning microphotolysis: a new photobleaching technique based on fast intensity modulation of a scanned laser beam and confocal imaging. J. Microsc. 176:23-33
98. Weiss S. 1999. Fluorescence spectroscopy of single biomolecules. Science 283:1676-83
99. Wente S. 2000. Gatekeepers of the nucleus. Science 288:1374-77