Optical single-channel analysis of the aerolysin pore in erythrocyte membranes

Biophysical Journal

Volumn 70, Issue 2 I, 1996, Pages 723-732

  Tschödrich Rotter, Mathias ^a   Kubitscheck, Ulrich ^a   Ugochukwu, Gabriele ^a   Thomas Buckley, J ^b   Peters, Reiner ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b UNIVERSITY OF VICTORIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AEROLYSIN; BACTERIAL TOXIN; UNCLASSIFIED DRUG;

AEROMONAS; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL DEATH; CHANNEL GATING; ERYTHROCYTE MEMBRANE; EXTRACELLULAR CALCIUM; MATHEMATICAL ANALYSIS; TOXICITY TESTING; TRANSPORT KINETICS;

AEROMONAS; BACTERIA (MICROORGANISMS); MYCTEROPERCA PHENAX;

EID: 0030032367     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79612-1     Document Type: Article

References (31)

1. Allbriton, N. L., T. Meyer, and L Stryer. 1992 Range of messenger action of calcium ion and inositol 1,4,5-triphosphate. Science. 258:1812-1815.
2. Axelrod, D., D. E. Koppel, J. Schlessinger, E. Elson, and W. W. Webb. 1976. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16:1055-1069.
3. Blonk, J. C. G., A. Don, H. van Alst. and J. J. Birmingham. 1993. Fluorescence photobleaching recovery in the confocal scanning microscope. J. Microsc. 169:363-374.
4. Buckley, J. T. 1990. Purification of cloned proaerolysin released by a low protease mutant of Aeromonas salmonicida, Biochem. Cell Biol. 68: 221-224.
5. Dennert, G., and E. R. Podack. 1983. Cytolysis by H-2-specific T killer cells. J. Exp. Med. 157:1483-1495.
6. Howard, S. P., and J. T. Buckley. 1982. Membrane glycoprotein receptor and hole-forming properties of a cytolytic protein toxin. Biochemistry. 21:1662-1667.
7. Jacobson, K., E. Wu, and G. Poste. 1976. Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching. Biochim. Biophys. Acta. 433:215-222.
8. Kaplan, J. H., B. Forbush, and J. F. Hoffman. 1978. Rapid photolytic release of adenosine 5′-triphosphate from a protected analogue: utilization by the Na:K pump of human red blood cell ghosts. Biochemistry. 17:1929-1935.
9. Kubitscheck, U., L. Pratsch, H. Passow, and R. Peters. 1995. Calcium pump kinetics determined in single erythrocyte ghosts by microphotolysis and confocal imaging. Biophys. J. 69:30-41.
10. Menestrina, G. 1994. Pore-forming toxins and antimicrobial polypeptides. Toxicol. Appl. Pharmacol. 87:1-268.
11. Nicotera, P., B. Zhivotovsky, and S. Orrenius. 1994. Nuclear calcium transport and the role of calcium in apoptosis. Cell Calcium. 16: 279-288.
12. Orrenius, S., D. J. McConkey, G. Bellomo, and P. Nicotera. 1989. Role of Ca2+ in toxic cell killing. Trends Biochem. Sci. 10:281-285.
13. Paine, P. L., and P. Scherr. 1975. Drag coefficients for the movement of rigid spheres through liquid-filled cylindrical pores. Biophys. J. 15: 1087-1091.
14. Pappenheimer, J. R., E. M. Renkin, and L. M. Borrero. 1951. Filtration, diffusion and molecular sieving through peripheral capillary membranes. Am. J. Physiol. 167:13-23.
15. Parker, M. W., J. T. Buckley, J. P. M. Postma, A. D. Tucker, K. Leonard, F. Pattus, and D. Tsernoglou. 1994. Structure of Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states. Nature. 367:292-295.
16. Peters, R. 1983. Nuclear envelope permeability measured by fluorescence microphotoiysis of single liver cell nuclei. J. Cell Biol. 258: 11427-11429.
17. Peters, R. 1984. Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis. EMBO J. 3:1831-1836.
18. Peters, R . J. Peters, K. H. Tews, and W. Bähr. 1974. A microfluorimetric study of translational diffusion in erythrocyte membranes. Biochim Biophys. Acta. 367:282-294.
19. Peters, R., H. Sauer, J- Tschopp, and G. Fritzsch. 1990. Transients of perform pore formation observed by fluorescence microscopic single channel recording. EMBO J. 9:2447-2451.
20. Peters, R., and M. Scholz. 1991. Fluorescence photobleaching techniques. In New Techniques of Optical Microscopy and Microspectroscopy. R. J. Cherry, editor. Macmillan Press, Houndsmill. 199-228.
21. Petersen, O. H., C. C. H. Petersen. and H. Kasai. 1994. Calcium and hormone action. Annu. Rev. Physiol. 56:297-319.
22. Renkin, E. M. 1954. Filtration, diffusion, and molecular sieving through porous cellulose membrane. J. Gen. Physiol. 38:225-243.
23. Sakmann, B., and E. Neher. 1991. Single-Channel Recording. Plenum Press, New York and London.
24. Sauer, H., L. Pratsch, G. Fritzsch, S. Bhakdi, and R. Peters. 1991. Complement pore genesis observed in erythrocyte membranes by fluorescence microscopic single-channel recording. Biochem. J. 276:395-399.
25. Schwoch, G., and H. Passow. 1973. Preparation and properties of human erythrocyte ghosts. Mol. Cell. Biochem. 2:197-218.
26. 2+ with trapped indicators. Trends Biochem. Sci. 263-266.
27. van der Goot, F. G., F. Pattus, M. Parker, and J. T. Buckley. 1994. The cytolytic toxin aerolysin. from the soluble form to the transmembrane channel. Toxicology. 87:19-28.
28. Wedekind, P., U. Kubitscheck, and R. Peters. 1994. Scanning microphotolysis: a new photobleaching technique based on fast intensity modulation of a scanned laser beam and confocal imaging. J. Microsc 176:23-33
29. Wilmsen, H. U., J. T. Buckley, and F. Pattus. 1991. Site-directed mutagenesis at histidines of aerolysin from Aeromonas hydrophila: a lipid planar bilayer study. Mol. Microbiol. 5:2745-2751.
30. Wilmsen, H. U., K. R. Leonard, W. Tichelaar, J. T. Buckley, and F. Pattus. 1992. The aerolysin membrane channel is formed by heptamerization of the monomer. EMBO J. 11:2457-2463.
31. Wilmsen, H. U., F. Pattus, and J. T. Buckley. 1990. Aerolysin. a hemolysin from Aeromonas hydrophilia, forms voltage-gated channels in planar lipid bilayers. J. Membr. Biol. 115:71-81.