Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export

Science

Volumn 276, Issue 5320, 1997, Pages 1845-1848

  Richards, Stephanie A ^a,c   Carey, Kimberly L ^a   Macara, Ian G ^b  

^a UNIVERSITY OF VERMONT   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; MUTANT PROTEIN; NUCLEAR PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL STRAIN BHK; HYDROLYSIS; INTRACELLULAR TRANSPORT; MICROINJECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT;

EID: 0030879014     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5320.1845     Document Type: Article

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11. Fluorescence distribution within cells was quantitated with Comos software on the Bio-Rad MC1000 confocal system. Pixel values were gathered by accumulation to peak intensity. Percentage of nuclear fluorescence was calculated as [Σnuclear pixels/ Σ(nuclear + cytoplasmic pixels)] × 100.
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23. We thank T. Nishimoto for antiserum to RCC1 and J. DeGregori for Fug1 cDNA. Supported by Department of Health and Human Services grant GM 50526 and NIH environmental pathology training grant EST3207122 (S.A.R.).