Nuclear transport kinetics in microarrays of nuclear envelope patches

Journal of Structural Biology

Volumn 140, Issue 1-3, 2002, Pages 268-278

  Peters, Reiner ^a   Coutavas, Elias ^b   Siebrasse, Jan Peter ^a  

^a INST OF REPRO MED OF THE UNIV   (Germany)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Confocal microscopy; Membrane transport kinetics; Microarray analysis; Nuclear pore complex; Nuclear translocation; Nucleocytoplasmic transport; Optical single transporter recording; OSTR; Photobleaching recovery

Indexed keywords

ACID PROTEIN;

ANIMAL CELL; CELL NUCLEUS MEMBRANE; CONFERENCE PAPER; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DNA MICROARRAY; NONHUMAN; PRIORITY JOURNAL; RIBOSOME; TRANSPORT KINETICS;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; BIOCHEMISTRY; CELL NUCLEUS; KINETICS; MICROSCOPY, CONFOCAL; MODELS, BIOLOGICAL; NUCLEAR ENVELOPE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OOCYTES; TIME FACTORS; XENOPUS;

EID: 12244311861     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(02)00525-7     Document Type: Conference Paper

References (72)

1. Adam, S.A., Stern-Marr, R., Gerace, L., 1990. Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell Biol. 111, 807-816.
2. Bayliss, B., Corbett, A.H., Stewart, M., 2000. The molecular mechanism of transport of macromolecules through nuclear pore complexes. Traffic 1, 448-456.
3. Ben-Efraim, I., Gerace, L., 2001. Gradient of increasing affinity of importin β for nucleoporins along the pathway of nuclear import. J. Cell Biol. 152, 411-417.
4. Bischoff, F.R., Ponstingl, H., 1995. Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1. Methods Enzymol. 257, 135-144.
5. Blobel, G., Potter, V.R., 1966. Nuclei from rat liver: Isolation method that combines purity with high yield. Science 154, 1662-1665.
6. Chook, Y.M., Blobel, G., 2001. Karyopherins and nuclear transport. Curr. Opin. Struct. Biol. 11, 703-715.
7. Englmeir, L., Olivo, J.C., Mattaj, I.W., 1999. Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis. Curr. Biol. 9, 0-41.
8. Finlay, D.R., Newmeyer, D.D., Price, T.M., Forbes, D.J., 1987. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores. J. Cell Biol. 104, 189-200.
9. Finlay, D.R., Forbes, D.J., 1990. Reconstitution of biochemically altered nuclear pores: Transport can be eliminated and restored. Cell 60, 17-29.
10. Finlay, D.R., Meier, E., Bradley, P., Horecka, J., Forbes, D.J., 1991. A complex of nuclear pore proteins required for pore function. J. Cell Biol. 114, 169-183.
11. Forbes, D.J., Kirschner, M.W., Newport, J.W., 1983. Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs. Cell 34, 13-23.
12. Grandi, P., Dang, T., Pane, N., Shevchenko, A., Mann, M., Forbes, D.J., Hurt, E., 1997. Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Mol. Biol. Cell 8, 2017-2038.
13. Greber, U.F., Gerace, L., 1995. Depletion of calcium from the lumen of the endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus. J. Cell Biol. 128, 5-14.
14. Görlich, D., Pante, N., Kutay, U., Aebi, U., Bischoff, F.R., 1996. Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15, 5584-5594.
15. Hassel, I., Cezanne, V., Trevino, C., Schlatter, H., Romero-Matuschek, I., Schmidt, A., Fasold, H., 1966. Export of ribosomal subunits from resealed rat liver nuclear envelopes. Eur. J. Biochem. 241, 32-37.
16. Hinshaw, J.E., Carragher, B.O., Milligan, R.A., 1992. Architecture and design of the nuclear pore complex. Cell 69, 1133-1144.
17. Holaska, J.M., Paschal, B.M., 1998. A cytosolic activity distinct from crm1 mediates nuclear export of protein kinase inhibitor in permeabilized cells. Proc. Natl. Acad. Sci. USA 95, 14739-14744.
18. Holaska, J.M., Wilson, K.L., Mansharamani, M., 2002. The nuclear envelope, lamins and nuclear assembly. Curr. Opin. Cell Biol. 14, 357-364.
19. Kehlenbach, R.H., Dickmanns, A., Gerace, L., 1998. Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT in vitro. J. Cell Biol. 141, 863-874.
20. Keminer, O., Peters, R., 1999. Permeability of single nuclear pores. Biophys. J. 77, 217-228.
21. Keminer, O., Siebrasse, J.P., Zerf, K., Peters, R., 1999. Optical recording of signal-mediated protein transport through single nuclear pore complexes. Proc. Natl. Acad. Sci. USA. 96, 11842-11847.
22. Kose, S., Imamato, N., Tachibana, T., Shimamoto, T., Yoneda, Y., 1997. Ran-unassisted nuclear migration of a 97-kDa component of nuclear pore-targeting complex. J. Cell Biol. 139, 841-849.
23. Kutay, U., Bischoff, F.R., Kostka, S., Kraft, R., Görlich, D., 1997. Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell 90, 1061-1071.
24. Lang, I., Scholz, M., Peters, R., 1986. Molecular mobility and nucleocytoplasmic flux in hepatoma cells. J. Cell Biol. 102, 1183-1190.
25. Lohka, M.J., Masui, Y., 1983. Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. Science 220, 719-721.
26. Macara, I.G., 2001. Transport into and out of the nucleus. Microbiol. Mol. Rev. 65, 570-594.
27. Middeler, G., Zerf, K., Jenovai, S., Thülig, A., Tschödrich-Rotter, M., Kubitscheck, U., Peters, R., 1997. The tumor suppressor p53 is subject to both nuclear import and export, and both are fast, energy-dependent and lectin-inhibited. Oncogene 14, 1407-1417.
28. Moore, M.S., Blobel, G., 1994. Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Natl. Acad. Sci. USA 91, 10212-10216.
29. Nachury, M.V., Weis, K., 1999. The direction of transport through the nuclear pore can be inverted. Proc. Natl. Acad. Sci. USA 96, 9622-9627.
30. Nakielny, S., Dreyfuss, G., 1998. Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis. Curr. Biol. 8, 89-95.
31. Nakielny, S., Dreyfuss, G., 1999. Transport of proteins and RNAs in and out of the nucleus. Cell 99, 677-690.
32. Nemergut, M.E., Macara, I.G., 2000. Nuclear import of the Ran exchange factor, RCC1, is mediated by at least two distinct mechanisms. J. Cell Biol. 140, 835-850.
33. Nevo, R., Markiewicz, P., Kapon, R., Elbaum, M., Reich, Z., 2000. High-resolution imaging of the nuclear pore complex by AC scanning force microscopy. Single Mol. 1, 109-114.
34. Newmeyer, D.D., Lucocq, J.M., Burglin, T.R., De Robertis, E.M., 1986a. Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation. EMBO J. 5, 501-510.
35. Newmeyer, D.D., Finlay, D.R., Forbes, D.J., 1986b. In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins. J. Cell Biol. 103, 2091-2102.
36. Newmeyer, D.D., Forbes, D.J., 1988. Nuclear import can be separated into distinct steps in vitro: Nuclear pore binding and translocation. Cell 52, 641-653.
37. Newmeyer, D.D., Wilson, K.L., 1991. Egg extracts for nuclear import and nuclear assembly reactions. Methods Cell Biol. 36, 607-634.
38. Ossareh-Nazari, B., Bachelerie, F., Dargemont, C., 1997. Evidence for a role of Crm1 in signal-mediated nuclear protein export. Science 278, 141-144.
39. Ossareh-Nazari, B., Maison, C., Black, B.E., Levesque, L., Pascal, B.M., Dargemont, C., 2000. RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro. Mol. Cell. Biol. 20, 4562-4571.
40. Paine, P.L., Moore, L.C., Horowitz, S.B., 1975. Nuclear envelope permeability. Nature 254, 109-114.
41. Paine, P.L., Austerberry, C.F., Desjarlais, L.J., Horowitz, S.B., 1983. Protein loss during nuclear isolation. J. Cell Biol. 97, 1240-1242.
42. Paschal, B.M., Gerace, L., 1995. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore protein p62. J. Cell Biol. 129, 925-937.
43. Peters, R., 1983. Nuclear envelope permeability measured by fluorescence microphotolysis of single liver cell nuclei. J. Biol. Chem. 258, 11427-11429.
44. Peters, R., 1984. Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis. EMBO J. 3, 1831-1836.
45. Peters, R., 1986. Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility. Biochim. Biophys. Acta 864, 305-359.
46. Peters, R., Passow, H., 1984. Anion transport in single erythrocyte ghosts measured by fluorescence microphotolysis. Biochim. Biophys. Acta 777, 334-338.
47. Peters, R., Peters, J., Tews, K.H., Bähr, W., 1974. A microfluorimetric study of translational diffusion in erythrocyte membranes. Biochim. Biophys. Acta 367, 282-294.
48. Peters, R., Sauer, H., Tschopp, J., Fritzsch, G., 1990. Transients of perforin pore formation observed by fluorescence microscopic single channel recording. EMBO J. 9, 2447-2451.
49. 2+ stores. Science 273, 1875-1877.
50. Powers, M.A., Macaulay, C., Masiarz, F.R., Forbes, D.J., 1995. Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication. J Cell Biol. 128, 721-736.
51. Rexach, M., Blobel, G., 1995. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83, 683-692.
52. Radtke, T., Schmalz, D., Coutavas, E., Soliman, T.M., Peters, R., 2001. Kinetics of protein import into isolated Xenopus oocyte nuclei. Proc. Natl. Acad. Sci. USA. 98, 2407-2412.
53. Radu, A., Moore, M.S., Blobel, G., 1995. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81, 215-222.
54. Rakowska, A., Danker, T., Schneider, S.W., Oberleithner, H., 1998. ATP-Induced shape change of nuclear pores visualized with the atomic force microscope. J. Membr. Biol. 163, 129-136.
55. Ribbeck, K., Lipowsky, G., Kent, H.M., Stewart, M., Görlich, D., 1998. NTF2 mediates nuclear import of Ran. EMBO J. 17, 6587-6598.
56. Ribbeck, K., Kutay, U., Parskava, E., Görlich, D., 1999. The translocation of transportin-cargo complexes through the nuclear pores is independent of both Ran and energy. Curr. Biol. 9, 47-50.
57. Ribbeck, K., Görlich, D., 2001. Kinetic analysis of translocation through nuclear pore complexes. EMBO J. 20, 1320-1330.
58. Rout, M.P., Aitchison, J.D., Suprapto, A., Hjertaas, K., Zhao, Y., Chait, B., 2000. The yeast nuclear pore complex: composition, Architecture and transport mechanism. J. Cell Biol. 148, 635-651.
59. Siebrasse, J.P., Peters, R., 2002. Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopes. EMBO Rep. 3, 887-892.
60. Siebrasse, J.P., Coutavas, E., Peters, R., 2002. Reconstitution of nuclear protein export in isolated nuclear envelopes. J. Cell Biol. 158, 849-854.
61. Smith, A., Brownawell, A., Macara, I., 1988. Nuclear import of Ran is mediated by the transport factor NTF2. Curr. Biol. 8, 1403-1406.
62. 2+ store. Science 270, 1835-1838.
63. Stoffler, D., Goldie, K.N., Feja, B., Aebi, U., 1999. Calcium-mediated structural changes of native nuclear pore complexes monitored by time-lapse atomic force microscopy. J. Mol. Biol. 287, 741-752.
64. Talcott, B., Moore, M.S., 1999. Getting across the nuclear pore complex. Trends Cell Biol. 9, 312-318.
65. Tschödrich-Rotter, M., Peters, R., 1998. An optical method for recording the activity of single transporters in membrane patches. J. Microsc. 192, 114-125.
66. Vancurova, I., Lou, W., Paine, T.M., Paine, P.L., 1993. Nucleoplasmin uptake by facilitated transport and intranuclear binding. Eur. J. Cell Biol. 62, 22-33.
67. Vasu, S.K., Forbes, D.J., 2001. Nuclear pores and nuclear assembly. Curr. Opin. Cell Biol. 13, 363-375.
68. Walther, T.C., Fornerod, M., Pickersgill, H., Goldberg, M., Allen, T.D., Mattaj, I.W., 2001. The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. EMBO J. 20, 5703-5714.
69. Walther, T.C., Pickersgill, H., Cordes, V.C., Goldberg, M., Allen, T.D., Mattaj, I.W., Fornerod, M., 2002. The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import. J. Cell Biol. 158, 63-67.
70. Wang, H., Clapham, D.E., 1999. Conformational changes of the in situ nuclear pore complex. Biophys. J. 77, 241-247.
71. Wente, S., 2000. Gatekeepers of the nucleus. Science 288, 1374-1377.
72. Zhang, C., Clarke, P.R., 2000. Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts. Science 288, 1429-1432.