Continuous scanning microphotolysis: A simple laser scanning microscopic method for lateral transport measurements employing single- or two-photon excitation

Bioimaging

Volumn 4, Issue 3, 1996, Pages 158-167

  Kubitscheck, Ulrich ^a   Heinrich, Oliver ^a   Peters, Reiner ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

confocal microscopy; continuous fluorescence microphotolysis; fluorescence photobleaching; lateral diffusion; two photon excitation

Indexed keywords

ARTICLE; BLEACHING; CONFOCAL MICROSCOPY; FLUORESCENCE; PRIORITY JOURNAL;

MYCTEROPERCA PHENAX;

EID: 0030497874     PISSN: 09669051     EISSN: None     Source Type: Journal    
DOI: 10.1002/1361-6374(199609)4:3<158::AID-BIO6>3.0.CO;2-C     Document Type: Article

References (13)

1. Axelrod D, Koppel D E, Schlessinger J, Elson E and Webb W W 1976 Mobility measurement by analysis of fluorescence photobleaching recovery kinetics Biophys. J. 16 1055-69
2. Berg H C 1983 Random Walks in Biology (Princeton, NJ: Princeton University Press)
3. Berland K M, So P T C and Gratton E 1995 Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment Biophys. J. 68 694-701
4. Born M and Wolf E 1986 Principles of Optics 6th edn (New York: Pergamon)
5. Brünger A, Peters R and Schulten K 1985 Continuous fluorescence microphotolysis to observe lateral diffusion in membranes: Theoretical methods and applications J. Chem. Phys. 82 2147-61
6. Denk W 1994 Two-photon scanning photochemical microscopy: Mapping ligand-gated ion channel distributions Proc. Natl Acad. Sci. USA 91 6629-33
7. Denk W, Strickler J H and Webb W W 1990 Two-photon laser scanning microscopy Science 248 445-58
8. Edidin M 1992 Translational diffusion of membrane proteins The Structure of Biological Membranes ed P Yeagle (Buffalo, NY: CRC Press) pp 539-72
9. Ficner R and Huber R 1993 Refined crystal structure of phycoerythrin from Porphyridium cruentum at 023-nm resolution and localization of the γ subunit Eur. J. Biochem. 218 103-6
10. Hell S W, Bahlmann K, Schrader M, Soini A, Malak H, Gryczynski I and Lakowicz J R 1996 Three-photon excitation in fluorescence microscopy J. Biomed. Opt. 1 71-4
11. Hell S and Stelzer E H K 1992 Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation Opt. Commun. 93 277-82
12. Ishihara A and Jacobson K 1993 A closer look at how membrane proteins move Biophys. J. 65 1754-55
13. Wedekind P, Kubitscheck U, Heinrich O and Peters R 1996 Line scanning microphotolysis for diffraction limited measurements of lateral diffusion Biophys. J. 71 1621-32