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Volumn 80, Issue 6, 2001, Pages 2954-2967

Visualization and tracking of single protein molecules in the cell nucleus

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BLEACHING; CELL NUCLEUS; CELL STRAIN 3T3; CHROMATOPHORE; CHROMOSOME STRUCTURE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN TRANSPORT; QUANTITATIVE ASSAY;

EID: 0035008139     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)76261-3     Document Type: Article
Times cited : (133)

References (55)
  • 1
    • 0031009827 scopus 로고    scopus 로고
    • Chromatin dynamics in interphase nuclei and its implications for nuclear structure
    • Abney, J. R., B. Cutler, M. L. Fillbach, D. Axelrod, and B. A. Scalettar. 1997. Chromatin dynamics in interphase nuclei and its implications for nuclear structure. J. Cell Biol. 137:1459-1468.
    • (1997) J. Cell Biol. , vol.137 , pp. 1459-1468
    • Abney, J.R.1    Cutler, B.2    Fillbach, M.L.3    Axelrod, D.4    Scalettar, B.A.5
  • 2
    • 0025083331 scopus 로고
    • Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors
    • Adam, S., R. Sterne-Marr, and L. Gerace. 1990. Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell Biol. 111:807-816.
    • (1990) J. Cell Biol. , vol.111 , pp. 807-816
    • Adam, S.1    Sterne-Marr, R.2    Gerace, L.3
  • 3
    • 0026528045 scopus 로고
    • Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4°C
    • Anderson, C. M., G. N. Georgiou, I. E. G. Morrison, G. V. W. Stevenson, and R. J. Cherry. 1992. Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4°C. J. Cell Sci. 101:415-425.
    • (1992) J. Cell Sci. , vol.101 , pp. 415-425
    • Anderson, C.M.1    Georgiou, G.N.2    Morrison, I.E.G.3    Stevenson, G.V.W.4    Cherry, R.J.5
  • 4
    • 0017192686 scopus 로고
    • Mobility measurement by analysis of fluorescence photobleaching recovery kinetics
    • Axelrod, D., D. E. Koppel, J. Schlessinger, E. Elson, and W. W. Webb. 1976. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16:1055-1069.
    • (1976) Biophys. J. , vol.16 , pp. 1055-1069
    • Axelrod, D.1    Koppel, D.E.2    Schlessinger, J.3    Elson, E.4    Webb, W.W.5
  • 5
    • 0004232671 scopus 로고
    • Princeton University Press, Princeton, New Jersey
    • Berg, H. C. 1983. Random Walks in Biology. Princeton University Press, Princeton, New Jersey.
    • (1983) Random Walks in Biology
    • Berg, H.C.1
  • 6
    • 0030964113 scopus 로고    scopus 로고
    • The dynamic nuclear redistribution of an hnRNP K-homologous protein during Drosophila embryo development and heat-shock. Flexibility of transcription sites in vivo
    • Buchenau, P., H. Saumweber, and D. J. Jovin. 1997. The dynamic nuclear redistribution of an hnRNP K-homologous protein during Drosophila embryo development and heat-shock. Flexibility of transcription sites in vivo. J. Cell Biol. 137:291-303.
    • (1997) J. Cell Biol. , vol.137 , pp. 291-303
    • Buchenau, P.1    Saumweber, H.2    Jovin, D.J.3
  • 7
    • 0000534160 scopus 로고
    • Position measurement with a resolution and noise-limited instrument
    • Bobroff, N. 1986. Position measurement with a resolution and noise-limited instrument. Rev. Sci. Instrum. 57:1152-1157.
    • (1986) Rev. Sci. Instrum. , vol.57 , pp. 1152-1157
    • Bobroff, N.1
  • 8
    • 0040307478 scopus 로고
    • Anomalous diffusion in disordered media: Statistical mechanisms, models, and physical applications
    • Bouchaud, J.-P., and A. Georges. 1990. Anomalous diffusion in disordered media: statistical mechanisms, models, and physical applications. Phys. Rep. 195:127-193.
    • (1990) Phys. Rep. , vol.195 , pp. 127-193
    • Bouchaud, J.-P.1    Georges, A.2
  • 9
    • 0027619735 scopus 로고
    • How to detect nonrandom motion of proteins in membranes
    • Cherry, R. 1993. How to detect nonrandom motion of proteins in membranes. Biophys. J. 64:1651-1652.
    • (1993) Biophys. J. , vol.64 , pp. 1651-1652
    • Cherry, R.1
  • 10
    • 0033519680 scopus 로고    scopus 로고
    • Pre-mRNP particles: From gene to nuclear pore
    • Daneholt, B. 1999. Pre-mRNP particles: from gene to nuclear pore. Curr. Biol. 9:R412-R415.
    • (1999) Curr. Biol. , vol.9
    • Daneholt, B.1
  • 12
    • 0017236539 scopus 로고
    • Measurement of membrane protein lateral diffusion in single cells
    • Edidin, M., Y. Zagyansky, and T. J. Lardner. 1976. Measurement of membrane protein lateral diffusion in single cells. Science. 191: 466-468.
    • (1976) Science , vol.191 , pp. 466-468
    • Edidin, M.1    Zagyansky, Y.2    Lardner, T.J.3
  • 13
    • 0016379116 scopus 로고
    • Fluorescence correlation spectroscopy. I. Conceptual basis and theory
    • Elson, E. L., and D. Magde. 1974. Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers. 13:1-27.
    • (1974) Biopolymers , vol.13 , pp. 1-27
    • Elson, E.L.1    Magde, D.2
  • 14
    • 0029946890 scopus 로고    scopus 로고
    • Constrained diffusion or immobile fraction on cell surfaces: A new interpretation
    • Feder, T. J., I. Brust-Mascher, J. P. Slattery, B. Baird, and W. W. Webb. 1996. Constrained diffusion or immobile fraction on cell surfaces: a new interpretation. Biophys. J. 70:2767-2773.
    • (1996) Biophys. J. , vol.70 , pp. 2767-2773
    • Feder, T.J.1    Brust-Mascher, I.2    Slattery, J.P.3    Baird, B.4    Webb, W.W.5
  • 15
    • 0027503192 scopus 로고
    • Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index
    • Hell, S., G. Reiner, C. Cremer, and E. H. K. Stelzer. 1993. Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J. Microsc. 169:391-405.
    • (1993) J. Microsc. , vol.169 , pp. 391-405
    • Hell, S.1    Reiner, G.2    Cremer, C.3    Stelzer, E.H.K.4
  • 16
    • 0003061529 scopus 로고    scopus 로고
    • Single molecule detection in life science
    • Ishii, Y., and T. Yanagida. 2000. Single molecule detection in life science. Single Mol. 1:5-16.
    • (2000) Single Mol. , vol.1 , pp. 5-16
    • Ishii, Y.1    Yanagida, T.2
  • 17
    • 0017641594 scopus 로고
    • Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching
    • Jacobson, K., Z. Derzko, E. S. Wu, Y. Hou, and G. Poste. 1976. Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching. J. Supramol. Struct. 5:565-576.
    • (1976) J. Supramol. Struct. , vol.5 , pp. 565-576
    • Jacobson, K.1    Derzko, Z.2    Wu, E.S.3    Hou, Y.4    Poste, G.5
  • 18
    • 0028178377 scopus 로고
    • Three-dimensional structure of β-galactosidase from E. coli
    • Jacobson, R. H., X-J. Zhang, R. F. DuBose, and B. W. Matthews. 1994. Three-dimensional structure of β-galactosidase from E. coli. Nature. 369:761-766.
    • (1994) Nature , vol.369 , pp. 761-766
    • Jacobson, R.H.1    Zhang, X.-J.2    Dubose, R.F.3    Matthews, B.W.4
  • 19
    • 0343729418 scopus 로고    scopus 로고
    • Imaging and tracking of single GFP molecules in solution
    • Kubitscheck, U., O. Kückmann, T. Kues, and R. Peters. 2000. Imaging and tracking of single GFP molecules in solution. Biophys. J. 78:2170-2179.
    • (2000) Biophys. J. , vol.78 , pp. 2170-2179
    • Kubitscheck, U.1    Kückmann, O.2    Kues, T.3    Peters, R.4
  • 20
    • 0032830913 scopus 로고    scopus 로고
    • Visualization of the nuclear pore complex and its distribution by confocal laser scanning microscopy
    • Kubitscheck, U., T. Kues, and R. Peters. 1999. Visualization of the nuclear pore complex and its distribution by confocal laser scanning microscopy. Methods Enzymol. 307:207-230.
    • (1999) Methods Enzymol , vol.307 , pp. 207-230
    • Kubitscheck, U.1    Kues, T.2    Peters, R.3
  • 21
    • 0029930929 scopus 로고    scopus 로고
    • Single nuclear pores visualized by confocal microscopy and image processing
    • Kubitscheck, U., P. Wedekind, O. Zeidler, M. Grote, and R. Peters. 1996. Single nuclear pores visualized by confocal microscopy and image processing. Biophys. J. 70:2067-2077.
    • (1996) Biophys. J. , vol.70 , pp. 2067-2077
    • Kubitscheck, U.1    Wedekind, P.2    Zeidler, O.3    Grote, M.4    Peters, R.5
  • 22
    • 0032562608 scopus 로고    scopus 로고
    • Structure and function of the nucleus
    • Lamond, A. I., and W. C. Earnshaw. 1998. Structure and function of the nucleus. Science 280:547-553.
    • (1998) Science , vol.280 , pp. 547-553
    • Lamond, A.I.1    Earnshaw, W.C.2
  • 23
    • 0022549772 scopus 로고
    • Molecular mobility and nucleocytoplasmic flux of macromolecules in hepatoma cells
    • Lang, I., M. Scholz, and R. Peters. 1986. Molecular mobility and nucleocytoplasmic flux of macromolecules in hepatoma cells. J. Cell Biol. 102:1183-1190.
    • (1986) J. Cell Biol. , vol.102 , pp. 1183-1190
    • Lang, I.1    Scholz, M.2    Peters, R.3
  • 24
    • 0025649160 scopus 로고
    • A view of interphase chromosomes
    • Manuelidis, L. 1990. A view of interphase chromosomes. Science. 250: 1533-1540.
    • (1990) Science , vol.250 , pp. 1533-1540
    • Manuelidis, L.1
  • 26
    • 0031707505 scopus 로고    scopus 로고
    • Nucleocytoplasmic transport: The soluble phase
    • Mattaj, I. W., and L. Englmeier. 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67:265-306.
    • (1998) Annu. Rev. Biochem. , vol.67 , pp. 265-306
    • Mattaj, I.W.1    Englmeier, L.2
  • 28
    • 0033021689 scopus 로고    scopus 로고
    • Resolution of fluorescence correlation measurements
    • Meseth, U., T. Wohland, R. Rigler, and H. Vogel. 1999. Resolution of fluorescence correlation measurements. Biophys. J. 76:1619-1631.
    • (1999) Biophys. J. , vol.76 , pp. 1619-1631
    • Meseth, U.1    Wohland, T.2    Rigler, R.3    Vogel, H.4
  • 29
    • 0026271470 scopus 로고
    • Egg extracts for nuclear import and nuclear assembly reactions
    • Newmeyer, D. D., and K. L. Wilson. 1991. Egg extracts for nuclear import and nuclear assembly reactions. Meth. Cell Biol. 36:607-635.
    • (1991) Meth. Cell Biol. , vol.36 , pp. 607-635
    • Newmeyer, D.D.1    Wilson, K.L.2
  • 31
    • 0033779678 scopus 로고    scopus 로고
    • Diffusional protein transport within the nucleus: A message in the medium
    • Pederson, T. 2000. Diffusional protein transport within the nucleus: a message in the medium. Nat. Cell Biol. 2:E73-E74.
    • (2000) Nat. Cell Biol. , vol.2
    • Pederson, T.1
  • 32
    • 0023055373 scopus 로고
    • Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility
    • Peters, R. 1986. Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility. Biochim. Biophys. Acta. 864: 305-359.
    • (1986) Biochim. Biophys. Acta. , vol.864 , pp. 305-359
    • Peters, R.1
  • 33
    • 0016272796 scopus 로고
    • A microfluorimetric study of translational diffusion in erythrocyte membranes
    • Peters, R., J. Peters, K. H. Tews, and W. Bähr. 1974. A microfluorimetric study of translational diffusion in erythrocyte membranes. Biochim. Biophys. Acta. 367:282-294.
    • (1974) Biochim. Biophys. Acta. , vol.367 , pp. 282-294
    • Peters, R.1    Peters, J.2    Tews, K.H.3    Bähr, W.4
  • 35
    • 0034611785 scopus 로고    scopus 로고
    • High mobility of proteins in the mammalian cell nucleus
    • Phair, R. D., and T. Misteli. 2000. High mobility of proteins in the mammalian cell nucleus. Nature. 404:604-609.
    • (2000) Nature , vol.404 , pp. 604-609
    • Phair, R.D.1    Misteli, T.2
  • 36
    • 0032568529 scopus 로고    scopus 로고
    • Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells
    • Politz, J. C., E. S. Browne, D. E. Wolf, and T. Pederson. 1998. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc. Natl. Acad. Sci. 95:6043-6048.
    • (1998) Proc. Natl. Acad. Sci. , vol.95 , pp. 6043-6048
    • Politz, J.C.1    Browne, E.S.2    Wolf, D.E.3    Pederson, T.4
  • 38
    • 0026026630 scopus 로고
    • The rate of nuclear cytoplasmic protein transport is determined by the casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen
    • Rihs, H.-P., D. A. Jans, and R. Peters. 1991. The rate of nuclear cytoplasmic protein transport is determined by the casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen. EMBO J. 10:633-639.
    • (1991) EMBO J. , vol.10 , pp. 633-639
    • Rihs, H.-P.1    Jans, D.A.2    Peters, R.3
  • 39
    • 0024414104 scopus 로고
    • Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian Virus 40 T-antigen
    • Rihs, H.-P., and R. Peters. 1989. Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian Virus 40 T-antigen. EMBO J. 8:1479-1484.
    • (1989) EMBO J. , vol.8 , pp. 1479-1484
    • Rihs, H.-P.1    Peters, R.2
  • 40
    • 0030953956 scopus 로고    scopus 로고
    • Single-particle tracking: The distribution of diffusion coefficients
    • Saxton, M. J. 1997. Single-particle tracking: the distribution of diffusion coefficients. Biophys. J. 72:1744-1753.
    • (1997) Biophys. J. , vol.72 , pp. 1744-1753
    • Saxton, M.J.1
  • 41
    • 0030956033 scopus 로고    scopus 로고
    • Single-particle tracking: Applications to membrane dynamics
    • Saxton, M. J., and K. Jacobson. 1997. Single-particle tracking: applications to membrane dynamics. Annu. Rev. Biophys. Biomol. Struct. 26: 373-399.
    • (1997) Annu. Rev. Biophys. Biomol. Struct. , vol.26 , pp. 373-399
    • Saxton, M.J.1    Jacobson, K.2
  • 42
    • 33750626346 scopus 로고
    • Characterization of photophysics and mobility of single molecules in a fluid lipid membrane
    • Schmidt, T., G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler. 1995. Characterization of photophysics and mobility of single molecules in a fluid lipid membrane. J. Phys. Chem. 99:17662-17668.
    • (1995) J. Phys. Chem. , vol.99 , pp. 17662-17668
    • Schmidt, T.1    Schütz, G.J.2    Baumgartner, W.3    Gruber, H.J.4    Schindler, H.5
  • 44
    • 0023425242 scopus 로고
    • Nucleocytoplasmic protein traffic in single mammalian cells studied by fluorescence microphotolysis
    • Schulz, B., and R. Peters. 1987. Nucleocytoplasmic protein traffic in single mammalian cells studied by fluorescence microphotolysis. Biochim. Biophys. Acta. 930:419-431.
    • (1987) Biochim. Biophys. Acta. , vol.930 , pp. 419-431
    • Schulz, B.1    Peters, R.2
  • 45
    • 0030742748 scopus 로고    scopus 로고
    • Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus
    • Seksek, O., J. Biwersi, and A. S. Verkman. 1997. Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus. J. Cell Biol. 138:131-142.
    • (1997) J. Cell Biol. , vol.138 , pp. 131-142
    • Seksek, O.1    Biwersi, J.2    Verkman, A.S.3
  • 46
    • 0029829004 scopus 로고    scopus 로고
    • Dynamic elastic behaviour of α-satellite DNA domains visualized in situ in living human cells
    • Shelby, R. D., K. M. Hahn, and K. Sullivan. 1996. Dynamic elastic behaviour of α-satellite DNA domains visualized in situ in living human cells. J. Cell Biol. 135:545-557.
    • (1996) J. Cell Biol. , vol.135 , pp. 545-557
    • Shelby, R.D.1    Hahn, K.M.2    Sullivan, K.3
  • 47
    • 0032998181 scopus 로고    scopus 로고
    • Anomalous diffusion of major histocompatibility complex class I molecules on HeLa cells determined by single particle tracking
    • Smith, P. R., I. E. G. Morrison, K. M. Wilson, N. Fernández, and R. J. Cherry. 1999. Anomalous diffusion of major histocompatibility complex class I molecules on HeLa cells determined by single particle tracking. Biophys. J. 76:3331-3344.
    • (1999) Biophys. J. , vol.76 , pp. 3331-3344
    • Smith, P.R.1    Morrison, I.E.G.2    Wilson, K.M.3    Fernández, N.4    Cherry, R.J.5
  • 48
    • 0029763319 scopus 로고    scopus 로고
    • Line scanning microphotolysis for diffraction limited measurements of lateral diffusion
    • Wedekind, P., U. Kubitscheck, O. Heinrich, and R. Peters. 1996. Line scanning microphotolysis for diffraction limited measurements of lateral diffusion. Biophys. J. 71:1621-1632.
    • (1996) Biophys. J. , vol.71 , pp. 1621-1632
    • Wedekind, P.1    Kubitscheck, U.2    Heinrich, O.3    Peters, R.4
  • 49
    • 0028149090 scopus 로고
    • Scanning microphotolysis: A new photobleaching technique based on fast intensity modulation of a scanned laser beam and confocal imaging
    • Wedekind, P., U. Kubitscheck, and R. Peters. 1994. Scanning microphotolysis: a new photobleaching technique based on fast intensity modulation of a scanned laser beam and confocal imaging. J. Microsc. 176:23-33.
    • (1994) J. Microsc. , vol.176 , pp. 23-33
    • Wedekind, P.1    Kubitscheck, U.2    Peters, R.3
  • 50
    • 0033548686 scopus 로고    scopus 로고
    • Fluorescence spectroscopy of single biomolecules
    • Weiss, S. 1999. Fluorescence spectroscopy of single biomolecules. Science. 283:1676-1683.
    • (1999) Science , vol.283 , pp. 1676-1683
    • Weiss, S.1
  • 51
    • 0029815535 scopus 로고    scopus 로고
    • Single particle tracking of cell-surface HLA-DR molecules using R-phycoerythrin labeled antibodies and fluorescence digital imaging
    • Wilson, K. M., I. E. G. Morrison, P. R. Smith, N. Fernández, and R. J. Cherry. 1996. Single particle tracking of cell-surface HLA-DR molecules using R-phycoerythrin labeled antibodies and fluorescence digital imaging. J. Cell Sci. 109:2101-2109.
    • (1996) J. Cell Sci. , vol.109 , pp. 2101-2109
    • Wilson, K.M.1    Morrison, I.E.G.2    Smith, P.R.3    Fernández, N.4    Cherry, R.J.5
  • 52
    • 0001468698 scopus 로고    scopus 로고
    • Optical studies of single molecules at room temperature
    • Xie, X. S., and J. K. Trautman. 1998. Optical studies of single molecules at room temperature. Annu. Rev. Phys. Chem. 49:441-480.
    • (1998) Annu. Rev. Phys. Chem. , vol.49 , pp. 441-480
    • Xie, X.S.1    Trautman, J.K.2
  • 53
    • 0029063587 scopus 로고
    • Visualisation of chromosomal domains with boundary element associated factor beaf-32
    • Zhao, K., C. M. Hart, and U. K. Laemli. 1995. Visualisation of chromosomal domains with boundary element associated factor beaf-32. Cell. 81:879-889.
    • (1995) Cell , vol.81 , pp. 879-889
    • Zhao, K.1    Hart, C.M.2    Laemli, U.K.3
  • 54
    • 0032560028 scopus 로고    scopus 로고
    • Cell nucleus: Chromosome dynamics in nuclei of living cells
    • Zink, D., and T. Cremer. 1998. Cell nucleus: chromosome dynamics in nuclei of living cells. Curr. Biol. 8:R321-R324.
    • (1998) Curr. Biol. , vol.8
    • Zink, D.1    Cremer, T.2


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