Establishment of an immortalized human hepatic stellate cell line to develop antifibrotic therapies

Cell Transplantation

Volumn 12, Issue 5, 2003, Pages 499-507

  Shibata, Norikuni ^a   Watanabe, Takamasa ^b   Okitsu, Teru ^b   Sakaguchi, Masakiyo ^b   Takesue, Michihiko ^a   Kunieda, Takemi ^a   Omoto, Kenji ^a   Yamamoto, Shinichiro ^a   Tanaka, Noriaki ^b   Kobayashi, Naoya ^b  

^a KAWASAKI MEDICAL SCHOOL   (Japan)

^b OKAYAMA UNIVERSITY   (Japan)

Author keywords

Human hepatic stellate cells; Human telomerase reverse transcriptase; Interferon ; Rapamycin; Type I collagen

Indexed keywords

ADENOVIRUS VECTOR; ALPHA ACTIN; ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN TYPE 1; GAMMA INTERFERON; GREEN FLUORESCENT PROTEIN; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; RAPAMYCIN; RNA; RNA DIRECTED DNA POLYMERASE; TELOMERASE; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL AGING; CELL IMMORTALIZATION; CELL LINE TWNT 4; CELL PROLIFERATION; CELL TRANSPLANTATION; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LIVER CELL CULTURE; LIVER FIBROSIS; LIVER TUMOR; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STELLATE CELL; WESTERN BLOTTING;

ADENOVIRIDAE;

EID: 0042163211     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/000000003108747064     Document Type: Article

References (45)

1. Bachem, M. G.; Meyer, D.; Melchior, R.; Sell, K. M.; Gressner, A. M. Activation of rat liver perisinusoidal lipocytes by transforming growth factors derived from myofibroblastlike cells. A potential mechanism of self perpetuation in liver fibrogenesis. J. Clin. Invest. 89:19-27; 1992.
2. Baroni, G. S.; D'Ambrosio, L.; Curto, P.; Casini, A.; Mancini, R.; Jezequel, A. M.; Benedetti, A. Interferon gamma decreases hepatic stellate cell activation and extracellular matrix deposition in rat liver fibrosis. Hepatology 23:1189-1199; 1996.
3. Benedetti, A.; Di Sario, A.; Casini, A.; Ridolfi, F.; Bendia, E.; Pigini, P.; Tonnini, C.; D'Ambrosio, L.; Feliciangeli, G.; Macarri, G.; Svegliati-Baroni, G. Inhibition of the NA (+)/H (+) exchanger reduces rat hepatic stellate cell activity and liver fibrosis: An in vitro and in vivo study. Gastroenterology 120:545-556; 2001.
4. Blomhoff, R.; Rasmussen, M.; Nilsson, A.; Norum, K. R.; Berg, T.; Blaner, W. S.; Kato, M.; Mertz, J. R.; Goodman, D. S.; Eriksson, U. Hepatic retinol metabolism. Distribution of retinoids, enzymes, and binding proteins in isolated rat liver cells. J. Biol. Chem. 260:13560-13565; 1985.
5. Brunn, G. J.; Hudson, C. C.; Sekulic, A.; Williams, J. M.; Hosoi, H.; Houghton, P. J.; Lawrence, J. C., Jr.; Abraham, R. T. Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin. Science 277:99-101; 1997.
6. Collins, K.; Kobayashi, R.; Greider, C. W. Purification of tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme. Cell 81:677-686; 1995.
7. Counter, C. M.; Avilion, A. A.; LeFeuvre, C. E.; Stewart, N. G.; Greider, C. W. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11:1921-1929; 1992.
8. Di Sario, A.; Bendia, E.; Svegliati Baroni, G.; Ridolfi, F.; Bolognini, L.; Feliciangeli, G.; Jezequel, A. M.; Orlandi, F.; Benedetti, A. Intracellular pathways mediating Na+/H+ exchange activation by platelet-derived growth factor in rat hepatic stellate cells. Gastroenterology 16:1155-1166; 1999.
9. Edwards, D. R.; Murphy, G.; Reynolds, J. J. Transforming growth factor modulates the expression of collagenase and metalloproteinase inhibitor. EMBO J. 6:1899-1904; 1987.
10. Failli, P.; DeFranco, R. M.; Caligiuri, A.; Gentilini, A.; Romanelli, R. G.; Marra, F.; Batignani, G.; Guerra, C. T.; Laffi, G.; Gentilini, P.; Pinzani, M. Nitrovasodilators inhibit platelet-derived growth factor-induced proliferation and migration of activated human hepatic stellate cells. Gastroenterology 119:479-492; 2000.
11. Fischer, R.; Schmitt, M.; Bode, J. G. Expression of the peripheral-type benzodiazepine receptor and apoptosis induction in hepatic stellate cells. Gastroenterology 120:1285-1288; 2001.
12. Geerts, A.; Vrijsen, R.; Rauterberg, J.; Burt, A.; Schellinck, P.; Wisse E. In vitro differentiation of fat storing cells parallels marked increase of collagen synthesis and secretion. J. Hepatol. 9:59-68; 1989.
13. Halvorsen, T. L.; Leibowitz, G.; Levine, F. Telomerase activity is sufficient to allow transformed cells to escape from crisis. Mol. Cell. Biol. 19:1864-1870; 1999.
14. Hautekeete, M. L.; Geerts, A. The hepatic stellate (Ito) cell: Its role in human liver disease. Virchows Arch. 430:195-207; 1997.
15. Hellemans, K.; Grinko, I.; Rombouts, K. Alltrans and 9-cis retinoic acid alter rat hepatic stellate cell phenotype differentially. Gut 45:134-142; 1999.
16. Hiyama, E.; Hiyama, K.; Yokohama, T.; Ichikawa, T.; Matsuura, Y. Length of telomeric repeats in neuroblastoma: Correlation with prognosis and other biological characteristics. Cancer Res. 83:159-164; 1992.
17. Hosoi, H.; Dilling, M. B.; Shikata, T.; Liu, L. N.; Shu, L.; Ashmun, R. A.; Germain, G. S.; Abraham, R. T.; Houghton, P. J. Rapamycin causes poorly reversible inhibition of mTOR and induces p53-indepent apoptosis in human rhabdomyosarcoma cells. Cancer Res. 59:886-894; 1999.
18. Hosoi, H.; Dilling, M. B.; Liu, L. N.; Danks, M. K.; Shikata, T.; Sekulic, A.; Abraham, R. T.; Lawrence, J. C., Jr.; Houghton, P. J. Studies on the mechanism of resistance to rapamycin in human cancer cells. Mol. Pharmacol. 54:815-824; 1998.
19. Issa, R.; Williams, E.; Tirm, N. Apoptosis of hepatic stellate cells: Involvement in resolution of biliary fibrosis and regulation by soluble growth factors. Gut 48:548-557; 2001.
20. Iwamoto, H.; Sakai, H.; Tada, S.; Nakamuta, M.; Nawata, H. Induction of apoptosis in rat hepatic stellate cells by disruption of integrin-mediated cell adhesion. J. Lab. Clin. Med. 134:83-89; 1999.
21. Kagawa, S.; Chao, H.; Jian, G.; Parericia, K.; Suk, J.; Roth, J. A.; Curley, S. A.; Stephens, C.; Fang, B. Antitumor activity and bystander effect of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) gene. Cancer Res. 61:3330-3338; 2001.
22. Kahari, V. M.; Chen, Y. Q.; Su, M. W.; Ramirez, F.; Uitto, J. Tumor necrosis factor-alpha and interferon-gamma suppressed the activation of human type I collagen gene expression by transforming growth factor-beta 1. Evidence for two distinct mechanisms of inhibition at the transcriptional and posttranscriptional levels. J. Clin. Invest. 86:1489-1495; 1990.
23. Kobayashi, N.; Fujiwara, T.; Westerman, K. A.; Inoue, Y.; Sakaguchi, M.; Noguchi, H.; Miyazaki, M.; et al. Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science 287:1258-1262; 2000.
24. Marra, F.; Efsen, E.; Romanelli, R. G.; Caligiuri, A.; Pastacaldi, S.; Batignani, G.; Bonacchi, A.; Caporale, R.; Laffi, G.; Pinzani, M.; Gentilini, P. Ligands of peroxisome proliferator-activated receptor gamma modulate profibrogenic and proinflammatory actions in hepatic stellate cells. Gastroenterology 119:466-478; 2000.
25. Milani, S.; Herbst, H.; Schuppan, D.; Hahn, E. G.; Stein, H. In situ hybridization for procollagen types I, III and IV mRNA in normal and fibrotic rat liver: Evidence for predominant expression in nonparenchymal liver cells. Hepatology 10:84-92; 1989.
26. Murakami, K.; Abe, T.; Miyazawa, M.; Yamaguchi, M.; Masuda, T.; Matsuura, T.; Nagamori, S.; Takeuchi, K.; Kyougoku, M. Establishment of a new human cell line, LI90, exhibiting characteristics of hepatic Ito (fat-storing) cell. Lab. Invest. 72:731-739; 1995.
27. Ogata, I.; Mochida, S.; Tomiya, T.; Fujiwara, K. Minor contribution of hepatocytes to collagen production in normal and early fibrotic rat livers. Hepatology 14:351-367; 1991.
28. Okuno, M.; Akita, K.; Moriwaki, H.; Kawada, N.; Ikeda, K.; Kaneda, K.; Suzuki, Y.; Kojima, S. Prevention of rat hepatic fibrosis by the protease inhibitor, nafamostat mesilate, via reduced generation of active TGF-beta. Gastroenterology 120:1784-1800; 2001.
29. Pinzani, M.; Gesukado, L.; Sabbah, G. M. Effects of platelet derived growth factor and other polypeptide mitogen on DNA synthesis and growth of rat liver fat storing cells. J. Clin. Invest. 84:1786-1793; 1989.
30. Rebollo, A.; Merida, I.; Gomez, J.; Pitton, C.; Silva, A.; Martinez, C.; Garcia, A. Differential effect of rapamycin and cyclosporin A in proliferation in a murine T cell line expressing either intermediate or high affinity receptor for IL-2. Cytokine 7:277-286; 1995.
31. Rockey, D. C.; Maher, J. J.; Jarnagin, W. R.; Gabbiani, G.; Friedman, S. L. Inhibition of rat hepatic lipocyte activation in culture by interferon-γ. Hepatology 16:776-784; 1992.
32. Sabers, C. J.; Martin, M. M.; Brunn, G. J.; Williams, J. M.; Dumont, F. J.; Wiederrecht, G.; Abraham, R. T. Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. J. Biol. Chem. 270:815-822; 1995.
33. Sakaguchi, M.; Miyazaki, M.; Inoue, Y.; Tsuji, T.; Kouchi, H.; Tanaka, T.; Yamada, H.; et al. Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts. J. Cell Biol. 149:1193-1202; 2000.
34. Sakaida, I.; Uchida, K.; Matsumura, Y.; Okita, K. Interferon gamma treatment prevents procollagen gene expression without affecting transforming growth factor beta1 expression in pig serum-induced rat liver fibrosis in vivo. J Hepatol. 28:471-479; 1998.
35. Shay, J. W.; Pereira-Smith, O. M.; Wright, W. E. A role for both Rb and p53 in the regulation of human cellular senescence. Exp. Cell Res. 196:33-39; 1991.
36. Shi, Y.; Frankel, A.; Radvanyi, L. G.; Penn, L. Z.; Miller, R. G.; Mills, G. B. Rapamycin enhances apoptosis and increase sensitivity to cisplatin in vitro. Cancer Res. 55:1982-1988; 1995.
37. Shimizu, I.; Mizobuchi, Y.; Yasuda, M.; Shiba, M.; Ma, Y. R.; Horie, T.; Liu, F.; Ito, S. Inhibitory effect of estradiol on activation of rat hepatic stellate cells in vivo and in vitro. Gut 44:127-136; 1999.
38. Sugawara, H.; Ueno, T.; Torimura, T. Inhibitory effect of OPC-15161, a component of fungus thielavia minor, on proliferation and extracellular matrix production of rat cultured hepatic stellate cells. J. Cell. Physiol. 174:398-406; 1998.
39. Tirm, N.; Morgan, S.; Evans, M. Hepatic stellate cells express the low affinity nerve growth factor receptor p75 and undergo apoptosis in response to nerve growth factor stimulation. Am. J. Pathol. 156:1235-1243; 2000.
40. Vaziri, H.; Dragowska, W.; Allscopp, R. C.; Thonas, T. E.; Harley, C. B. ; Lansdorp, P. M. Evidence for a mitotic clock in human hematopoietic stem cells: Loss of telomeric DNA with age. Proc. Natl. Acad. Sci. USA 91:9857-9860; 1994.
41. Watanabe, T.; Shibata, N.; Westerman, K. A. Establishment of immortalized activated hepatic stellate cells to develop bioartificial livers. Transplantation (in press).
42. Westerman, K. A.; Leboulch, P. Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93:8971-8976; 1996.
43. Wright, M. C.; Issa, R.; Smart, D. E.; Trim, N.; Murray, G. I.; Primrose, J. N.; Arthur, M. J.; Iredale, J. P.; Mann, D. A. Gliotoxin stimulates the apoptosis of human and rat hepatic stellate cells and enhances the resolution of liver fibrosis in rats. Gastroenterology 121:685-698; 2001.
44. Yoshiji, H.; Kuriyama, S.; Yoshiji, J.; Yoshiji, H.; Kuriyama, S.; Yoshii, J.; Ikenaka, Y.; Noguchi, R.; Nakatani, T.; Tsujinoue, H.; Fukui, H. Angiotensin-II type I receptor interaction is a major regulator for liver fibrosis development in rat. Hepatology 34:745-750; 2001.
45. Zhu, J.; Wu, J.; Frizell, E.; Liu, S.; Bashey, R.; Rubin, R.; Norton, P. ; Zern, M. Rapamycin inhibits hepatic stellate cell proliferation in vitro and limits fibrogenesis in an in vivo model of liver fibrosis. Gastroenterology 117:1198-1204; 1999.