Demonstration of the importance and usefulness of manipulating non-active-site residues in protein design

Journal of Biochemistry

Volumn 129, Issue 6, 2001, Pages 943-948

  Shimotohno, Akie ^a,b   Oue, Shinya ^b   Yano, Takato ^b   Kuramitsu, Seiki ^a   Kagamiyama, Hiroyuki ^b  

^a OSAKA UNIVERSITY   (Japan)

^b OSAKA MEDICAL COLLEGE   (Japan)

Author keywords

Directed evolution; Domain motion; Protein design; Protein engineering

Indexed keywords

AMINO ACID; ASPARTATE AMINOTRANSFERASE;

ARTICLE; BACTERIUM MUTANT; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME STRUCTURE; GENE CLUSTER; GENE MUTATION; GENETIC ENGINEERING; PROTEIN DOMAIN; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS);

EID: 0034956003     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002941     Document Type: Article

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