-
1
-
-
0037212977
-
Role of endothelial cells in early pancreas and liver development
-
Lammert E., Cleaver O., Melton D. Role of endothelial cells in early pancreas and liver development. Mech Dev. 120:2003;59-64.
-
(2003)
Mech Dev
, vol.120
, pp. 59-64
-
-
Lammert, E.1
Cleaver, O.2
Melton, D.3
-
2
-
-
0036439461
-
Signaling pathways in vascular development
-
Rossant J., Howard L. Signaling pathways in vascular development. Annu Rev Cell Dev Biol. 18:2002;541-573.
-
(2002)
Annu Rev Cell Dev Biol
, vol.18
, pp. 541-573
-
-
Rossant, J.1
Howard, L.2
-
3
-
-
0033135876
-
Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development
-
Gale N.W., Yancopoulos G.D. Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development. Genes Dev. 13:1999;1055-1066.
-
(1999)
Genes Dev
, vol.13
, pp. 1055-1066
-
-
Gale, N.W.1
Yancopoulos, G.D.2
-
4
-
-
0030728938
-
Ephrins and their receptors: A repulsive topic?
-
Gale N.W., Yancopoulos G.D. Ephrins and their receptors: a repulsive topic? Cell Tissue Res. 290:1997;227-241.
-
(1997)
Cell Tissue Res
, vol.290
, pp. 227-241
-
-
Gale, N.W.1
Yancopoulos, G.D.2
-
6
-
-
0036303033
-
Mechanisms and functions of Eph and ephrin signalling
-
Kullander K., Klein R. Mechanisms and functions of Eph and ephrin signalling. Nat Rev Mol Cell Biol. 3:2002;475-486.
-
(2002)
Nat Rev Mol Cell Biol
, vol.3
, pp. 475-486
-
-
Kullander, K.1
Klein, R.2
-
7
-
-
0032577446
-
Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4
-
Wang H.U., Chen Z.F., Anderson D.J. Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 93:1998;741-753.
-
(1998)
Cell
, vol.93
, pp. 741-753
-
-
Wang, H.U.1
Chen, Z.F.2
Anderson, D.J.3
-
8
-
-
0033197535
-
Symmetrical mutant phenotypes of the receptor EphB4 and its specific transmembrane ligand ephrin-B2 in cardiovascular development
-
Gerety S.S., Wang H.U., Chen Z.F., Anderson D.J. Symmetrical mutant phenotypes of the receptor EphB4 and its specific transmembrane ligand ephrin-B2 in cardiovascular development. Mol Cell. 4:1999;403-414.
-
(1999)
Mol Cell
, vol.4
, pp. 403-414
-
-
Gerety, S.S.1
Wang, H.U.2
Chen, Z.F.3
Anderson, D.J.4
-
9
-
-
0035846911
-
The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration
-
By making a targeted deletion of the cytoplasmic tail of ephrinB2, this study provides in vivo evidence for the importance of reverse ephrin signaling in vascular development.
-
Adams R.H., Diella F., Hennig S., Helmbacher F., Deutsch U., Klein R. The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration. Cell. 104:2001;57-69 By making a targeted deletion of the cytoplasmic tail of ephrinB2, this study provides in vivo evidence for the importance of reverse ephrin signaling in vascular development.
-
(2001)
Cell
, vol.104
, pp. 57-69
-
-
Adams, R.H.1
Diella, F.2
Hennig, S.3
Helmbacher, F.4
Deutsch, U.5
Klein, R.6
-
10
-
-
0036337787
-
Cardiovascular ephrinB2 function is essential for embryonic angiogenesis
-
Gerety S.S., Anderson D.J. Cardiovascular ephrinB2 function is essential for embryonic angiogenesis. Development. 129:2002;1397-1410.
-
(2002)
Development
, vol.129
, pp. 1397-1410
-
-
Gerety, S.S.1
Anderson, D.J.2
-
11
-
-
0034817456
-
Vascular expression of Notch pathway receptors and ligands is restricted to arterial vessels
-
Villa N., Walker L., Lindsell C.E., Gasson J., Iruela-Arispe M.L., Weinmaster G. Vascular expression of Notch pathway receptors and ligands is restricted to arterial vessels. Mech Dev. 108:2001;161-164.
-
(2001)
Mech Dev
, vol.108
, pp. 161-164
-
-
Villa, N.1
Walker, L.2
Lindsell, C.E.3
Gasson, J.4
Iruela-Arispe, M.L.5
Weinmaster, G.6
-
13
-
-
0035829517
-
Gridlock signalling pathway fashions the first embryonic artery
-
•], this paper provides evidence for the importance of Notch signaling in artery/vein specification in zebrafish. In addition, they show that the Hey gene, gridlock, is needed to correctly specify the dorsal aorta and may be downstream of Notch signaling.
-
•], this paper provides evidence for the importance of Notch signaling in artery/vein specification in zebrafish. In addition, they show that the Hey gene, gridlock, is needed to correctly specify the dorsal aorta and may be downstream of Notch signaling.
-
(2001)
Nature
, vol.414
, pp. 216-220
-
-
Zhong, T.P.1
Childs, S.2
Leu, J.P.3
Fishman, M.C.4
-
14
-
-
0034629325
-
Gridlock, an HLH gene required for assembly of the aorta in zebrafish
-
Zhong T.P., Rosenberg M., Mohideen M.A., Weinstein B., Fishman M.C. gridlock, an HLH gene required for assembly of the aorta in zebrafish. Science. 287:2000;1820-1824.
-
(2000)
Science
, vol.287
, pp. 1820-1824
-
-
Zhong, T.P.1
Rosenberg, M.2
Mohideen, M.A.3
Weinstein, B.4
Fishman, M.C.5
-
15
-
-
0034610274
-
Members of the HRT family of basic helix-loop-helix proteins act as transcriptional repressors downstream of Notch signaling
-
Nakagawa O., McFadden D.G., Nakagawa M., Yanagisawa H., Hu T., Srivastava D., Olson E.N. Members of the HRT family of basic helix-loop-helix proteins act as transcriptional repressors downstream of Notch signaling. Proc Natl Acad Sci USA. 97:2000;13655-13660.
-
(2000)
Proc Natl Acad Sci USA
, vol.97
, pp. 13655-13660
-
-
Nakagawa, O.1
McFadden, D.G.2
Nakagawa, M.3
Yanagisawa, H.4
Hu, T.5
Srivastava, D.6
Olson, E.N.7
-
16
-
-
0037125916
-
-/- mice
-
-/- mice. Curr Biol. 12:2002;1601-1604.
-
(2002)
Curr Biol
, vol.12
, pp. 1601-1604
-
-
Gessler, M.1
Knobeloch, K.P.2
Helisch, A.3
Amann, K.4
Schumacher, N.5
Rohde, E.6
Fischer, A.7
Leimeister, C.8
-
17
-
-
0037125957
-
Tetralogy of fallot and other congenital heart defects in Hey2 mutant mice
-
Donovan J., Kordylewska A., Jan Y.N., Utset M.F. Tetralogy of fallot and other congenital heart defects in Hey2 mutant mice. Curr Biol. 12:2002;1605-1610.
-
(2002)
Curr Biol
, vol.12
, pp. 1605-1610
-
-
Donovan, J.1
Kordylewska, A.2
Jan, Y.N.3
Utset, M.F.4
-
18
-
-
0037058935
-
Ventricular septal defect and cardiomyopathy in mice lacking the transcription factor CHF1/Hey2
-
Sakata Y., Kamei C.N., Nakagami H., Bronson R., Liao J.K., Chin M.T. Ventricular septal defect and cardiomyopathy in mice lacking the transcription factor CHF1/Hey2. Proc Natl Acad Sci USA. 99:2002;16197-16202.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 16197-16202
-
-
Sakata, Y.1
Kamei, C.N.2
Nakagami, H.3
Bronson, R.4
Liao, J.K.5
Chin, M.T.6
-
19
-
-
0036733639
-
Arteries and veins: Making a difference with zebrafish
-
Lawson N.D., Weinstein B.M. Arteries and veins: making a difference with zebrafish. Nat Rev Genet. 3:2002;674-682.
-
(2002)
Nat Rev Genet
, vol.3
, pp. 674-682
-
-
Lawson, N.D.1
Weinstein, B.M.2
-
20
-
-
0036070186
-
Sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
-
An excellent study using injection of mRNA or antisense morpholinos into transgenic and mutant zebrafish to establish a genetic hierarchy of action of sonic hedgehog, VEGF and Notch in artery differentiation.
-
Lawson N.D., Vogel A.M., Weinstein B.M. sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation. Dev Cell. 3:2002;127-136 An excellent study using injection of mRNA or antisense morpholinos into transgenic and mutant zebrafish to establish a genetic hierarchy of action of sonic hedgehog, VEGF and Notch in artery differentiation.
-
(2002)
Dev Cell
, vol.3
, pp. 127-136
-
-
Lawson, N.D.1
Vogel, A.M.2
Weinstein, B.M.3
-
21
-
-
0035143709
-
Differential expression of VEGF isoforms in mouse during development and in the adult
-
Ng Y.S., Rohan R., Sunday M.E., Demello D.E., D'Amore P.A. Differential expression of VEGF isoforms in mouse during development and in the adult. Dev Dyn. 220:2001;112-121.
-
(2001)
Dev Dyn
, vol.220
, pp. 112-121
-
-
Ng, Y.S.1
Rohan, R.2
Sunday, M.E.3
Demello, D.E.4
D'Amore, P.A.5
-
22
-
-
0036174063
-
Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms
-
Stalmans I., Ng Y.S., Rohan R., Fruttiger M., Bouche A., Yuce A., Fujisawa H., Hermans B., Shani M., Jansen S.et al. Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms. J Clin Invest. 109:2002;327-336.
-
(2002)
J Clin Invest
, vol.109
, pp. 327-336
-
-
Stalmans, I.1
Ng, Y.S.2
Rohan, R.3
Fruttiger, M.4
Bouche, A.5
Yuce, A.6
Fujisawa, H.7
Hermans, B.8
Shani, M.9
Jansen, S.10
-
23
-
-
0037062491
-
Orchestration of angiogenesis and arteriovenous contribution by angiopoietins and vascular endothelial growth factor (VEGF)
-
Visconti R.P., Richardson C.D., Sato T.N. Orchestration of angiogenesis and arteriovenous contribution by angiopoietins and vascular endothelial growth factor (VEGF). Proc Natl Acad Sci USA. 99:2002;8219-8224.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 8219-8224
-
-
Visconti, R.P.1
Richardson, C.D.2
Sato, T.N.3
-
24
-
-
0037077134
-
Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin
-
This paper is the first demonstration that signals from sensory nerves induce arterial endothelial cell differentiation in embryonic mouse skin. Mutant mice defective in peripheral nerve formation are used elegantly to show the intimate developmental correlation between nerves and arteries.
-
Mukouyama Y.S., Shin D., Britsch S., Taniguchi M., Anderson D.J. Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin. Cell. 109:2002;693-705 This paper is the first demonstration that signals from sensory nerves induce arterial endothelial cell differentiation in embryonic mouse skin. Mutant mice defective in peripheral nerve formation are used elegantly to show the intimate developmental correlation between nerves and arteries.
-
(2002)
Cell
, vol.109
, pp. 693-705
-
-
Mukouyama, Y.S.1
Shin, D.2
Britsch, S.3
Taniguchi, M.4
Anderson, D.J.5
-
25
-
-
0037216703
-
Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: Implications for modulating arteriogenesis and angiogenesis
-
Liu Z.J., Shirakawa T., Li Y., Soma A., Oka M., Dotto G.P., Fairman R.M., Velazquez O.C., Herlyn M. Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol Cell Biol. 23:2003;14-25.
-
(2003)
Mol Cell Biol
, vol.23
, pp. 14-25
-
-
Liu, Z.J.1
Shirakawa, T.2
Li, Y.3
Soma, A.4
Oka, M.5
Dotto, G.P.6
Fairman, R.M.7
Velazquez, O.C.8
Herlyn, M.9
-
26
-
-
0034756449
-
Differential expression of neuropilin-1 and neuropilin-2 in arteries and veins
-
Herzog Y., Kalcheim C., Kahane N., Reshef R., Neufeld G. Differential expression of neuropilin-1 and neuropilin-2 in arteries and veins. Mech Dev. 109:2001;115-119.
-
(2001)
Mech Dev
, vol.109
, pp. 115-119
-
-
Herzog, Y.1
Kalcheim, C.2
Kahane, N.3
Reshef, R.4
Neufeld, G.5
-
27
-
-
0036803751
-
Abnormal lymphatic vessel development in neuropilin 2 mutant mice
-
Insertional mutation of the NP-2 gene results in loss of small lymphatic vessels and capillaries, whereas arteries, veins and larger, collecting lymphatic vessels develop normally. The authors suggest a specific role for NP-2, presumably acting with VEGFR3, in promoting the branching morphogenesis of small lymphatics.
-
Yuan L., Moyon D., Pardanaud L., Breant C., Karkkainen M.J., Alitalo K., Eichmann A. Abnormal lymphatic vessel development in neuropilin 2 mutant mice. Development. 129:2002;4797-4806 Insertional mutation of the NP-2 gene results in loss of small lymphatic vessels and capillaries, whereas arteries, veins and larger, collecting lymphatic vessels develop normally. The authors suggest a specific role for NP-2, presumably acting with VEGFR3, in promoting the branching morphogenesis of small lymphatics.
-
(2002)
Development
, vol.129
, pp. 4797-4806
-
-
Yuan, L.1
Moyon, D.2
Pardanaud, L.3
Breant, C.4
Karkkainen, M.J.5
Alitalo, K.6
Eichmann, A.7
-
28
-
-
0037133617
-
Targeting of both mouse neuropilin-1 and neuropilin-2 genes severely impairs developmental yolk sac and embryonic angiogenesis
-
Double mutants of NP-1 and NP-2 show very severe vascular defects resembling loss of VEGF or VEGFR2 function. Thus neuropilin signaling may be an obligate part of the VEGF signaling mechanism.
-
Takashima S., Kitakaze M., Asakura M., Asanuma H., Sanada S., Tashiro F., Niwa H., Miyazaki Ji J., Hirota S., Kitamura Y.et al. Targeting of both mouse neuropilin-1 and neuropilin-2 genes severely impairs developmental yolk sac and embryonic angiogenesis. Proc Natl Acad Sci USA. 99:2002;3657-3662 Double mutants of NP-1 and NP-2 show very severe vascular defects resembling loss of VEGF or VEGFR2 function. Thus neuropilin signaling may be an obligate part of the VEGF signaling mechanism.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 3657-3662
-
-
Takashima, S.1
Kitakaze, M.2
Asakura, M.3
Asanuma, H.4
Sanada, S.5
Tashiro, F.6
Niwa, H.7
Miyazaki Ji, J.8
Hirota, S.9
Kitamura, Y.10
-
29
-
-
0032549799
-
Neuropilin-1 is expressed by endothelial and tumor cells as an isoform- specific receptor for vascular endothelial growth factor
-
Soker S., Takashima S., Miao H.Q., Neufeld G., Klagsbrun M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform- specific receptor for vascular endothelial growth factor. Cell. 92:1998;735-745.
-
(1998)
Cell
, vol.92
, pp. 735-745
-
-
Soker, S.1
Takashima, S.2
Miao, H.Q.3
Neufeld, G.4
Klagsbrun, M.5
-
30
-
-
0034960583
-
The morphogen Sonic hedgehog is an indirect angiogenic agent upregulating two families of angiogenic growth factors
-
Sonic hedgehog protein is shown to induce robust formation of new blood vessels in a mouse tissue ischemia model. This is the first evidence that Shh could act as an indirect angiogenic factor by inducing VEGF and angiopoietin expression. Whether the effect is artery-specific is not addressed in this study.
-
Pola R., Ling L.E., Silver M., Corbley M.J., Kearney M., Blake Pepinsky R., Shapiro R., Taylor F.R., Baker D.P., Asahara T.et al. The morphogen Sonic hedgehog is an indirect angiogenic agent upregulating two families of angiogenic growth factors. Nat Med. 7:2001;706-711 Sonic hedgehog protein is shown to induce robust formation of new blood vessels in a mouse tissue ischemia model. This is the first evidence that Shh could act as an indirect angiogenic factor by inducing VEGF and angiopoietin expression. Whether the effect is artery-specific is not addressed in this study.
-
(2001)
Nat Med
, vol.7
, pp. 706-711
-
-
Pola, R.1
Ling, L.E.2
Silver, M.3
Corbley, M.J.4
Kearney, M.5
Blake Pepinsky, R.6
Shapiro, R.7
Taylor, F.R.8
Baker, D.P.9
Asahara, T.10
-
31
-
-
0035122695
-
Inhibition of lymphangiogenesis with resulting lymphedema in transgenic mice expressing soluble VEGF receptor-3
-
Because knockout of the lymphatic-specific VEGF receptor, VEGFR3, causes early lethality before establishment of the lymphatic system, the authors address the later role of VEGFR3 by expressing an inhibitory form of the receptor in the skin. Extensive loss of lymphatic vessels occurs, whereas the rest of the circulatory system is normal.
-
Makinen T., Jussila L., Veikkola T., Karpanen T., Kettunen M.I., Pulkkanen K.J., Kauppinen R., Jackson D.G., Kubo H., Nishikawa S.et al. Inhibition of lymphangiogenesis with resulting lymphedema in transgenic mice expressing soluble VEGF receptor-3. Nat Med. 7:2001;199-205 Because knockout of the lymphatic-specific VEGF receptor, VEGFR3, causes early lethality before establishment of the lymphatic system, the authors address the later role of VEGFR3 by expressing an inhibitory form of the receptor in the skin. Extensive loss of lymphatic vessels occurs, whereas the rest of the circulatory system is normal.
-
(2001)
Nat Med
, vol.7
, pp. 199-205
-
-
Makinen, T.1
Jussila, L.2
Veikkola, T.3
Karpanen, T.4
Kettunen, M.I.5
Pulkkanen, K.J.6
Kauppinen, R.7
Jackson, D.G.8
Kubo, H.9
Nishikawa, S.10
-
32
-
-
17744396472
-
Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice
-
Veikkola T., Jussila L., Makinen T., Karpanen T., Jeltsch M., Petrova T.V., Kubo H., Thurston G., McDonald D.M., Achen M.G.et al. Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice. EMBO J. 20:2001;1223-1231.
-
(2001)
EMBO J
, vol.20
, pp. 1223-1231
-
-
Veikkola, T.1
Jussila, L.2
Makinen, T.3
Karpanen, T.4
Jeltsch, M.5
Petrova, T.V.6
Kubo, H.7
Thurston, G.8
McDonald, D.M.9
Achen, M.G.10
-
33
-
-
18644382318
-
Angiopoietin-2 is required for postnatal angiogenesis and lymphatic patterning, and only the latter role is rescued by Angiopoietin-1
-
The authors demonstrate an essential role for Ang2 in retinal vascular development and lymphatic patterning. This is the first demonstration that angiopoietin is involved in the remodeling and maturation of lymphatic vasculature as is the case for blood vessels.
-
Gale N.W., Thurston G., Hackett S.F., Renard R., Wang Q., McClain J., Martin C., Witte C., Witte M.H., Jackson D.et al. Angiopoietin-2 is required for postnatal angiogenesis and lymphatic patterning, and only the latter role is rescued by Angiopoietin-1. Dev Cell. 3:2002;411-423 The authors demonstrate an essential role for Ang2 in retinal vascular development and lymphatic patterning. This is the first demonstration that angiopoietin is involved in the remodeling and maturation of lymphatic vasculature as is the case for blood vessels.
-
(2002)
Dev Cell
, vol.3
, pp. 411-423
-
-
Gale, N.W.1
Thurston, G.2
Hackett, S.F.3
Renard, R.4
Wang, Q.5
McClain, J.6
Martin, C.7
Witte, C.8
Witte, M.H.9
Jackson, D.10
-
34
-
-
0037428102
-
Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk
-
Abtahian F., Guerriero A., Sebzda E., Lu M.M., Zhou R., Mocsai A., Myers E.E., Huang B., Jackson D.G., Ferrari V.A.et al. Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk. Science. 299:2003;247-251.
-
(2003)
Science
, vol.299
, pp. 247-251
-
-
Abtahian, F.1
Guerriero, A.2
Sebzda, E.3
Lu, M.M.4
Zhou, R.5
Mocsai, A.6
Myers, E.E.7
Huang, B.8
Jackson, D.G.9
Ferrari, V.A.10
-
35
-
-
0033578853
-
Prox1 function is required for the development of the murine lymphatic system
-
Wigle J.T., Oliver G. Prox1 function is required for the development of the murine lymphatic system. Cell. 98:1999;769-778.
-
(1999)
Cell
, vol.98
, pp. 769-778
-
-
Wigle, J.T.1
Oliver, G.2
-
36
-
-
0037007216
-
An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype
-
•].
-
•].
-
(2002)
EMBO J
, vol.21
, pp. 1505-1513
-
-
Wigle, J.T.1
Harvey, N.2
Detmar, M.3
Lagutina, I.4
Grosveld, G.5
Gunn, M.D.6
Jackson, D.G.7
Oliver, G.8
-
37
-
-
18544386859
-
Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox-1 homeobox transcription factor
-
•].
-
•].
-
(2002)
EMBO J
, vol.21
, pp. 4593-4599
-
-
Petrova, T.V.1
Makinen, T.2
Makela, T.P.3
Saarela, J.4
Virtanen, I.5
Ferrell, R.E.6
Finegold, D.N.7
Kerjaschki, D.8
Yla-Herttuala, S.9
Alitalo, K.10
-
38
-
-
0036840138
-
Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate
-
•] report loss-of-function or gain-of-function studies of Prox-1 and demonstrate that Prox-1 is a key transcription factor specifying lymphatic endothelial cell fate.
-
•] report loss-of-function or gain-of-function studies of Prox-1 and demonstrate that Prox-1 is a key transcription factor specifying lymphatic endothelial cell fate.
-
(2002)
Dev Dyn
, vol.225
, pp. 351-357
-
-
Hong, Y.K.1
Harvey, N.2
Noh, Y.H.3
Schacht, V.4
Hirakawa, S.5
Detmar, M.6
Oliver, G.7
-
39
-
-
0036208217
-
The rediscovery of the lymphatic system: Old and new insights into the development and biological function of the lymphatic vasculature
-
Oliver G., Detmar M. The rediscovery of the lymphatic system: old and new insights into the development and biological function of the lymphatic vasculature. Genes Dev. 16:2002;773-783.
-
(2002)
Genes Dev
, vol.16
, pp. 773-783
-
-
Oliver, G.1
Detmar, M.2
|