Real time quantitative PCR and RT-PCR for analysis of Pneumocystis carinii hominis

Journal of Microbiological Methods

Volumn 45, Issue 2, 2001, Pages 113-118

  Kaiser, Karine ^a   Rabodonirina, Meja ^a   Picot, Stéphane ^a  

^a HOSPICES CIVILS DE LYON   (France)

Author keywords

Pneumocystis carinii hominis; Quantification assay; Quantitative PCR; Quantitative RT PCR; Viability assay

Indexed keywords

MESSENGER RNA; PROTOZOAL DNA;

ANALYTIC METHOD; ARTICLE; CELL CYCLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA DETERMINATION; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; CDC2 PROTEIN KINASE; DNA, FUNGAL; FLUORESCENT DYES; GENES, CDC; HUMANS; MICROSCOPY, FLUORESCENCE; ORGANIC CHEMICALS; PNEUMOCYSTIS; PNEUMONIA, PNEUMOCYSTIS; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TETRAHYDROFOLATE DEHYDROGENASE;

FUNGI; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS JIROVECII;

EID: 0035050954     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(01)00241-X     Document Type: Article

References (9)

1. New rat model of Pneumocystis carinii pneumonia
2. Diagnostic strategies in Pneumocystis carinii pneumonia
3. Identification of extrapulmonary Pneumocystis carinii in immunocompromised rats by PCR
4. Culture of Pneumocystis carinii sp. f hominis
5. Evidence for cdc2 gene in Pneumocystis carinii hominis and its implication for culture
6. Continuous axenic cultivation of Pneumocystis carinii
7. Use of semiquantitative PCR to assess onset and treatment of Pneumocystis carinii infection in the rat model
8. Pneumocystis carinii contains a functional cell-division-cycle cdc2 homologue
9. Amplification of mitochondrial ribosomal RNA sequences from Pneumocystis carinii DNA of rat and human origin