Recovery of microalgal biomass and metabolites: Process options and economics

Biotechnology Advances

Volumn 20, Issue 7-8, 2003, Pages 491-515

  Molina Grima, E ^a   Belarbi, E H ^a   Acien Fernandez F G ^a   Robles Medina, A ^a   Chisti, Yusuf ^b  

^a UNIVERSITY OF ALMERÍA   (Spain)

^b MASSEY UNIVERSITY   (New Zealand)

Author keywords

Biomass recovery; Eicosapentaenoic acid; Flocculation; Microalgae; Phaeodactylum tricornutum; Process economics

Indexed keywords

BIOMASS; BIOREACTORS;

PHOTOBIOREACTORS;

METABOLITES;

BIOTECHNOLOGY;

PHAEODACTYLUM TRICORNUTUM;

EID: 0037214545     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0734-9750(02)00050-2     Document Type: Article

References (82)

1. Acién Fernández F.G., García F., Sánchez J.A., Fernández J.M., Molina E. Modelling of biomass productivity in tubular photobioreactors for microalgal cultures: effects of dilution rate, tube diameter and solar irradiance. Biotechnol. Bioeng. 58:1998;605-616.
2. Acién Fernández F.G., Fernández Sevilla J.M., Sánchez Pérez J.A., Molina Grima E., Chisti Y. Airlift-driven external-loop tubular photobioreactors for outdoor production of microalgae: assessment of design and performance. Chem. Eng. Sci. 56:2001;2721-2732.
3. Banerjee A., Sharma R., Chisti Y., Banerjee U.C. Botryococcus braunii: a renewable source of hydrocarbons and other chemicals. Crit. Rev. Biotechnol. 22:2002;245-279.
4. Belarbi H., Molina E., Chisti Y. A process for high and scaleable recovery of high purity eicosapentaenoic acid esters from microalgae and fish oil. Enzyme Microb. Technol. 26:2000;516-529.
5. Belter P.A., Cussler E.L., Hu W.S. Bioseparations: Downstream Processing for Biotechnology. 1988;Wiley, New York.
6. Ben-Amotz A., Avron M. The biotechnology of mass culturing of Dunaliella for products of commercial interest. Cresswell R.C., Rees T.A.V., Shah N. Algal and cyanobacterial technology. 1987;90-114 Longman, London.
7. Benemann J.R., Kopman B.L., Weissman D.E., Eisenberg D.E., Goebel R.P. Development of microalgae harvesting and high rate pond technologies in California. Shelef G., Soeder C.J. Algal biomass. 1980;457 Elsevier, Amsterdam.
8. Bermejo Román R., Talavera E.M., Alvarez-Pez J.M. Chromatographic purification and characterization of b-phycoerythrin from Porphyridium cruentum. Semipreparative HPLC separation and characterization of its subunits. J. Chromatogr. A. 917:2001;135-145.
9. Bermejo Román R., Alvárez-Pez J.M., Acién Fernández F.G., Molina Grima E. Recovery of pure b-phycoerythrin from the microalga Porphyridium cruentum. J. Biotechnol. 93:2002;73-85.
10. Bilanovic D., Shelef G., Sukenik A. Flocculation of microalgae with cationic polymers - effects of medium salinity. Biomass. 17:1988;65-76.
11. Blanchemain A., Grizeau D. Increased production of eicosapentaenoic acid by Skeletonema costatum cells after decantation at low temperature. Biotechnol. Tech. 13:1999;497-501.
12. Borowitzka M.A. Microalgae as sources of fine chemicals. Microbiol. Sci. 3:1986;372-375.
13. Borowitzka M.A. Closed algal photobioreactors: design considerations for large-scale systems. J. Mar. Biotechnol. 4:1996;185-191.
14. Borowitzka M.A. Microalgae for aquaculture: opportunities and constraints. J. Appl. Phycol. 9:1997;393-401.
15. Borowitzka M.A. Commercial production of microalgae: ponds, tanks, tubes and fermenters. J. Biotechnol. 70:1999;313-321.
16. Bubrick P. Production of astaxanthin from Haematococcus. Biores. Technol. 38:1991;237-239.
17. Cannell R.J.P. Algal biotechnology. Appl. Biochem. Biotechnol. 26:1990;85-105.
18. Cartens M., Molina E., Robles A., Giménez A., Ibáñez M.J. Eicosapentaenoic acid (20:4n-3) from the marine microalga Phaeodactylum tricornutum. J. Am. Oil Chem. Soc. 73:1996;1025-1031.
19. Chisti Y. Strategies in downstream processing. Subramanian G. Bioseparation and bioprocessing: a handbook. vol. 2:1998;3-30 Wiley-VCH, New York.
20. Chisti Y. Shear sensitivity. Flickinger M.C., Drew S.W. Encyclopedia of bioprocess technology: fermentation, biocatalysis, and bioseparation. vol. 5:1999;2379-2406 Wiley, New York.
21. Chisti Y., Moo-Young M. Disruption of microbial cells for intracellular products. Enzyme Microb. Technol. 8:1986;194-204.
22. Chisti Y., Moo-Young M. Fermentation technology, bioprocessing, scale-up and manufacture. Moses M., Cape R.E. Biotechnology: the science and the business. 1991;167-209 Harwood Academic Publishers, New York.
23. Cohen J.M., Rourke G.A., Woodward R.L. Natural and synthetic polyelectrolytes as coagulants and coagulant aids. Bull. U. S. Dept. Health, Educ. Welf. 1957;. Robert A Taft Sanitary Eng Center, Cinncinati, OH, December.
24. Dodd J.C. Algae production and harvesting from animal wastewaters. Agric. Wastes. 1:1979;23-37.
25. D'Souza F.M.L., Lecossois D., Heasman M.P., Diemar J.A., Jackson C.J., Pendrey R.C. Evaluation of centrifuged microalgae concentrates as diets for Penaeus monodon Fabricius larvae. Aquacult. Res. 31:2000;661-670.
26. Dunstan G.A., Vokman J.K., Jeffrey S.W., Barret S.M. Biochemical composition of microalgae from the green algal classes Chlorophyceae and Parasinophyceae: 2. Lipid classes and fatty acid. J. Exp. Mar. Biol. Ecol. 161:1992;115-134.
27. Elmaleh S., Coma J., Grasmick A., Bourgade L. Magnesium induced algal flocculation in a fluidized bed. Water Sci. Technol. 23:1991;1695-1702.
28. Giménez Giménez A., Ibáñez M.J., Robles A., Molina E., García S., Esteban L. Downstream processing and purification of eicosapentaenoic (20:5n-3) and arachidonic acids (20:4n-6) from the microalga Porphyridium cruentum. Bioseparation. 7:1998;89-99.
29. Golueke C.G., Oswald W.J. Harvesting and processing sewage grown planktonic algae. J. Water Pollut. Control Fed. 37:1965;471-498.
30. Gudin C., Chaumont D. Cell fragility, the key problem of microalgae mass production in closed photobioreactors. Bioresour. Technol. 38:1991;141-151.
31. Gudin C., Therpenier C. Bioconversion of solar energy into organic chemicals by microalgae. Adv. Biotechnol. Processes. 6:1986;73-110.
32. Heasman M., Diemar J., O' Connor W., Sushames T., Foulkes L., Nell J.A. Development of extended shelf-life microalgae concentrate diets harvested by centrifugation for bivalve molluscs - a summary. Aquacult. Res. 31(8-9):2000;637-659.
33. Hedenskog G., Enebo L., Vendlová J., Prokes B.T.I. Investigation of some methods for increasing the digestibility in vitro of microalgae. Biotechnol. Bioeng. 11:1969;37-51.
34. Hee-Mock O., Lee S.J., Park M.H., Kim H.S., Kim H.C., Yoon J.H.et al. Harvesting of Chlorella vulgaris using a bioflocculant from Paenibacillus sp. AM49. Biotechnol. Lett. 23:2001;1229-1234.
35. Humphreys K. Jelen's cost and optimization engineering. 3rd ed. 1991;McGraw-Hill, New York.
36. Ibáñez González M.J., Robles Medina A., Molina Grima E., Giménez Giménez A., Carstens M., Esteban Cerdan L. Optimization of fatty acid extraction from Phaeodactylum tricornutum UTEX 640 biomass. J. Am. Oil Chem. Soc. 75:1998;1735-1740.
37. Jiang J.-Q., Graham N.J.D., Harward C. Comparison of polyferric sulphate with other coagulants for the removal of algae and algae-derived organic matter. Water Sci. Technol. 27:1993;221-230.
38. Kaya V.M., Picard G. Stability of chitosan gel as entrapment matrix of viable Scenedesmus bicellularis cells immobilized on screens for tertiary treatment of wastewater. Bioresour. Technol. 56:1996;147-155.
39. Koopman B., Lincoln E.P. Autoflotation harvesting of algae from high rate pond effluents. Agric. Wastes. 5:1983;231-246.
40. Lavoie A., de la Noue J. Harvesting microalgae with chitosan. J. World Maricult. Soc. 14:1983;685-694.
41. Lee S.J., Kim S.B., Kim J.E., Kwon G.S., Yoon B.D., Oh H.M. Effects of harvesting method and growth stage on the flocculation of the green alga Botryococcus braunii. Lett. Appl. Microbiol. 27:1998;14-18.
42. Li H.B., Chen F., Zhang T.Y., Yang F.Q., Xu G.Q. Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography. J. Chromatogr. A. 905:2001;151-155.
43. Lim G.B., Lee S.Y., Lee E.K., Haam S.J., Kim W.S. Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction. Biochem. Eng. J. 11:2002;181-187.
44. Lincoln E.P. Resource recovery with microalgae. Arch. Hydrobiol. 20:1985;25-34.
45. Lubian L.M. Concentrating cultured marine microalgae with chitosan. Aquacult. Eng. 8:1989;257-265.
46. MacKay D., Salusbury T. Choosing between centrifugation and crossflow microfiltration. Chem. Eng. (Lond.). 477:1988;45-50.
47. McCausland M.A., Brown M.R., Barrett S.M., Diemar J.A., Heasman M.P. Evaluation of live microalgae and microbial pastes as supplementary food for juvenile Pacific oyster (Crassostrea gigas). Aquaculture. 174:1999;323-342.
48. McGarry M.G. Alga flocculation with aluminium sulfate and polyelectrolytes. J. Water Pollut. Control Fed. 42:1970;R191-R201.
49. Mendes-Pinto M.M., Raposo M.F.J., Bowen J., Young A.J., Morais R. Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability. J. Appl. Phycol. 13:2001;19-24.
50. Middelberg A.P.J. The release of intracellular bioproducts. Subramanian G. Bioseparation and bioprocessing: a handbook. vol. 2:1994;131-164 Wiley-VCH, Weinheim.
51. Mohn F.H. Improved technologies for the harvesting and processing of microalgae and their impact on production costs. Arch. Hydrobiol., Beih. Ergeb. Limnol. 1:1978;228-253.
52. Mohn F.H. Experiences and strategies in the recovery of biomass from mass cultures of microalgae. Shelef G., Soeder C.J. Algae biomass. 1980;547-571 Elsevier, Amsterdam.
53. Molina Grima E. Microalgae, mass culture methods. Flickinger M.C., Drew S.W. Encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation. vol. 3:1999;1753-1769 Wiley, New York.
54. Molina Grima E., Robles Medina A., Giménez Giménez A., Sánchez Pérez J.A., García Camacho F., García Sánchez J.L. Comparison between extraction of lipids and fatty acids from microalgal biomass. J. Am. Oil Chem. Soc. 71:1994;955-959.
55. Molina Grima E., Robles Medina A., Giménez Giménez A., Ibañez González M.J. Gram-scale purification of eicosapentaenoic acid (EPA 20:5n3) from wet Phaeodactylum tricornutum UTEX 640 biomass. J. Appl. Phycol. 8:1996;359-367.
56. Molina Grima E., Acién Fernández F.G., Garcia Camacho F., Chisti Y. Photobioreactors: light regime, mass transfer, and scale-up. J. Biotechnol. 70:1999;231-248.
57. Moraine R., Shelef G., Sandbank F., Bar-Moshe Z., Shvartzbard I. Recovery of sewage borne algae: flocculation and centrifugation technique. Shelef G., Soeder C.J. Algae biomass. 1980;531-546 Elsevier, Amsterdam.
58. Morales J., de la Noue J., Picard G. Harvesting marine microalgae species by chitosan flocculation. Aquaculture Eng. 4:1985;257-270.
59. Nigam B.P., Ramanathan P.K., Venkataraman L.V. Application of chitosan as a flocculant for the cultures of the green alga: Scenedesmus acutus. Arch. Hydrobiol. 88:1980;378-387.
60. Peet M., Brind J., Ramchand C.N., Shah S., Vankar G.K. Two double-blind placebo-controlled pilot studies of eicosapentaenoic acid in the treatment of schizophrenia. Schizophr. Res. 49:2001;243-251.
61. Peet M., Horrobin D.F. A dose-ranging exploratory study of the effects of ethyl-eicosapentaenoate in patients with persistent schizophrenic symptoms. J. Psychiatr. Res. 36:2002;7-18.
62. Peters M.S., Timmerhaus K.D. Plant design and economics for chemical engineers. 4th ed. 1991;McGraw-Hill, New York.
63. Petrusevski B., Bolier G., Van Bremen A.N., Alaerts G.J. Tangential flow filtration: a method to concentrate freshwater algae. Water Res. 29:1995;1419-1424.
64. Pulz O. Photobioreactors: production systems for phototrophic microorganisms. Appl. Microbiol. Biotechnol. 57:2001;287-293.
65. Pulz O., Scheinbenbogen K. Photobioreactors: design and performance with respect to light energy input. Adv. Biochem. Eng. Biotechnol. 59:1998;123-152.
66. Pulz O., Scheibenbogen K., Gross W. Biotechnology with cyanobacteria and microalgae. Rehm H.-J., Reed G. Biotechnology. vol. 10:2001;105-136 Wiley-VCH, Weinheim.
67. Pushparaj B., Pelosi E., Torzillo G., Materassi R. Microbial biomass recovery using a synthetic cationic polymer. Bioresour. Technol. 43:1993;59-62.
68. Richmond A. Microalgae of economic potential. Richmond A. CRC handbook of microalgal mass culture. 1986;199-243 CRC Press, Boca Raton.
69. Richmond A., Becker E.W. Technological aspects of mass cultivation, a general outline. Richmond A. CRC handbook of microalgal mass culture. 1986;245-263 CRC Press, Boca Raton.
70. Robles Medina A., Giménez Giménez A., García Camacho F., Sánchez Pérez J.A., Molina Grima E., Contreras Gómez A. Concentration and purification of stearidonic, eicosapentaenoic, and docosahexaenoic acids from cod liver oil and the marine microalga Isochrysis galbana. J. Am. Oil Chem. Soc. 72:1995;575-583.
71. Rodríguez-Ruiz J., Belarbi E.-H., García Sánchez J.L., López Alonso D. Rapid simultaneous lipids extraction and transesterification for fatty acid analysis. Biotechnol. Tech. 12:1998;689-691.
72. Rossignol N., Vandanjon L., Jaouen P., Quemeneur F. Membrane technology for the continuous separation microalgae/culture medium: compared performances of cross-flow microfiltration and ultrafiltration. Aquacult. Eng. 20:1999;191-208.
73. Ruane, M. Extraction of caroteniferous materials from algae. Australian patent No. 7,239,574 (1977).
74. Sánchez Mirón A., Contreras Gómez A., García Camacho F., Molina Grima E., Chisti Y. Comparative evaluation of compact photobioreactors for large-scale monoculture of microalgae. J. Biotechnol. 70:1999;249-270.
75. Shelef G. Photosynthetic biomass production from sewage. Arch. Hydrobiol. Beih. 11:1978;3-14.
76. Suh H.H., Kwon G.S., Lee C.H., Kim H.S., Oh H.M., Yoon B.D. Characterization of bioflocculant produced by Bacillus sp. DP-152. J. Ferment. Bioeng. 84:1997;108-112.
77. Tenney M.W., Echelberger W.F., Schuessler R.G., Pavoni J.L. Algal flocculation with synthetic organic polyelectrolytes. Appl. Bacteriol. 18:1969;965-971.
78. Terry K.L., Raymond L.P. System design for the autotrophic production of microalgae. Enzyme Microb. Technol. 7:1985;474-487.
79. Tilton R.C., Murphy J., Dixon J.K. The flocculation of algae with synthetic polymeric flocculants. Water Res. 6:1972;155-164.
80. Tredici M.R. Bioreactors, photo. Flickinger M.C., Drew S.W. Encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation. vol. 1:1999;395-419 Wiley, New York.
81. Venkataraman L.V. New possibility for microalgae production and utilisation in India. Arch. Hydrobiol. Beih. 11:1978;199-210.
82. Yahi H., Elmaleh S., Coma J. Algal flocculation-sedimentation by pH increase in a continuous reactor. Water Sci. Technol. 30:1994;259-267.