Optimization of fatty acid extraction from Phaeodactylum tricornutum UTEX 640 biomass

JAOCS, Journal of the American Oil Chemists' Society

Volumn 75, Issue 12, 1998, Pages 1735-1740

  Ibanez Gonzalez M J ^a   Robles Medina, A ^a   Molina Grima, E ^a   Gimenez Gimenez A ^a   Carstens, M ^b   Esteban Cerdan L ^a  

^a UNIVERSITY OF ALMERÍA   (Spain)

^b UNIVERSIDAD NACIONAL DE LA PATAGONIA SAN JUAN BOSCO   (Argentina)

Author keywords

Eicosapentaenoic acid (EPA); Fatty acid extraction; Fatty acid purification; Phaeodactylum tricornutum

Indexed keywords

ETHANOL; EXTRACTION; FATTY ACIDS; LIPIDS; PARAFFINS; PH; POTASSIUM COMPOUNDS; PURIFICATION; SAPONIFICATION;

CAROTENOID; HEXANE; POTASSIUM HYDROXIDE;

BIOMASS;

EID: 0032315985     PISSN: 0003021X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11746-998-0325-z     Document Type: Article

References (18)

1. Bajpai, P., and P.K. Bajpai, Eicosapentaenoic Acid (EPA) Production from Microorganisms: A Review, J. Biotechnol. 30: 161-183 (1993).
2. Connor, W.E., The Importance of n-3 Fatty Acids in Health and Disease, Conference on Highly Unsaturated Fatty Acids in Nutrition and Disease Prevention, Abstract of International Conference on Highly Unsaturated Fatty Acids in Nutrition and Disease Prevention, Chairmen: W.E. Connor and R, Muggli, Sponsored by F. Hoffman-La Roche Ltd., Basel, Switzerland. Conference celebrated in Barcelona, Spain, 4-6 November, 1996, p. 1.
3. Radwan, S.S., Sources of C20-Polyunsaturated Fatty Acids for Biotechnological Use, Appl. Microbiol. Biotechnol. 35:421-430 (1991).
4. Simopoulos, A.P., Omega-3 Fatty Acids in Health and Disease and in Growth and Development, Am. J. Clin. Nutr. 54:438-463 (1991).
5. Rambjor, G.S., A.I. Walen, S.L. Windsor, and W.S. Harris, Eicosapentaenoic Acid Is Primarily Responsible for Hypotriglyceridemic Effect of Fish Oil in Humans, Lipids 31 (suppl.S):45-49 (1996).
6. Braden, L.M., and K.K. Carroll, Dietary Polyunsaturated Fat in Relation to Mammary Carcinogenesis in Rats, Ibid. 21:285-288 (1986).
7. Yongmanitchai, W., and O.P. Ward, Screening of Algae for Potential Alternative Sources of Eicosapentaenoic Acid, Phytochemistry 30:2963-2967 (1991).
8. Gartens, M., E. Molina Grima, A. Robles Medina, A. Giménez Giménez, and J. Ibáñez González, Eicosapentaenoic Acid (20:5n-3) from the Marine Microalga Phaeodactylum tricornutum, J. Am. Oil Chem. Soc. 73:1025-1031 (1996).
9. Robles Medina, A., A. Giménez Giménez, F. García Camacho, J.A. Sánchez Pérez, E. Molina Grima, and A. Contreras Gómez, Concentration and Purification of Stearidonic, Eicosapentaenoic, and Docosahexaenoic Acids from Cod Liver Oil and the Marine Microalga Isochrysis galhana, Ibid. 72:575-583 (1995).
10. Molina Grima, E., A. Robles Medina, A. Giménez Giménez, and M.J. Ibáñez Gonzãlez, Gram-Scale Purification of Eicosapentaenoic Acid (EPA. 20:5n-3) from Wet Phaeodactylum tricornutum UTEX 640 Biomass, J. Appl. Phycol. 8:359-367 (1996).
11. Giménez Giménez, A. M.J. Ibáñez González, A. Robles Medina, E. Molina Grima, S. Garcia Salas, and L. Estebán Cerdán, Downstream Processing and Purification of Eicosapentaenoic (20:5n-3) and Arachidonic Acids (20:4n-6) from the Microalga Porphyridium cruentum, Bioseparation 7:89-99 (1998).
12. Whyte, J.N., Biochemical Composition and Energy Content of Six Species of Phytoplankton Used in Mariculture of Bivalves, Aquaculture 60:231-241 (1987).
13. Lepage, G., and C.C. Roy, Improved Recovery of Fatty Acid Through Direct Transesterification Without Prior Extraction or Purification, J. Lipid Res. 25:1391-1396 (1984).
14. Molina Grima, E., A. Robles Medina, A. Giménez Giménez, J.A. Sánchez Pérez, F. García Camacho, and J.L., García Sánchez, Comparison Between Extraction of Lipids and Fatty Acids from Microalgal Biomass, J. Am. Oil Chem. Soc. 71:955-959 (1994).
15. Lowry, O., N. Rosebrough, A. Farr, and R. Randall, Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem. 193:265-275 (1951).
16. Hansmann, E., Pigment Analysis, in Handbook of Phycological Methods, Culture Methods and Growth Measurements, edited by J.R. Stein, Cambridge University Press, Cambridge, 1973, pp. 359-368.
17. Coulson, J.M., and J.F. Richardson, Ingeniería Química. Operaciones Básicas, Reverté, Barcelona, 1988. tomo II, pp. 777-778.
18. Bligh, E.G., and W.J. Dyer, A Rapid Method of Total Lipid Extraction and Purification, Can, J. Biochem. Physiol. 37:911-917 (1959).