Study of adenovirus production in serum-free 293SF suspension culture by GFP-expression monitoring

Biotechnology Progress

Volumn 13, Issue 6, 1997, Pages 709-714

  Côté, Johanne ^a   Bourget, Lucie ^a   Garnier, Alain ^a,b   Kamen, Amine ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; BIOASSAY; FLUORESCENCE; PARTICLES (PARTICULATE MATTER); PROTEINS;

ADENOVIRUS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEIN;

VIRUSES;

GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN;

ADENOVIRUS; ARTICLE; CELL LINE; CELL SURVIVAL; CULTURE MEDIUM; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; KINETICS; VIRION;

ADENOVIRIDAE; CELL LINE; CELL SURVIVAL; CULTURE MEDIA, SERUM-FREE; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; KINETICS; LUMINESCENT PROTEINS; RECOMBINANT PROTEINS; VIRION;

ADENOVIRIDAE; ANIMALIA;

EID: 18544403142     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp970110i     Document Type: Article

References (26)

1. Acsadi, G.; Jani, A.; Massie, B.; Simoneau, M.; Holland, P.; Blaschuk, K.; Karpati, G. A differential efficiency of adenovirus-mediated in vivo gene transfer into skeletal muscle cells of different maturity. Hum. Mol. Genet. 1994, 3, 579-584.
2. Acsadi, G.; Lochmüller, H.; Jani, A.; Huard, J.; Massie, B.; Prescott, S.; Simoneau, M.; Petrof, B. J.; Karpati, G. Dystrophin expression in muscles of mdx mice after adenovirus-mediated in vivo gene transfer. Hum. Gene Ther. 1996, 7, 129-140.
3. Berg, D. T.; McClure, D. B.; Grinnell, B. W. High-level expression of secreted proteins from cells adapted to serum-free suspension culture. BioTechniques 1993, 14, 972-978.
4. Chalfie, M.; Tu, Y.; Euskirchen, G.; Ward, W. W., Prasher, D. C. Green fluorescent protein as a marker for gene expression. Science 1994, 263, 802-805.
5. Cheng, L.; Fu, J.; Tsukamoto, A.; Hawley, R. G. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells. Nat. Biotechnol. 1996, 14, 606-609.
6. Côté, J., Garnier, A., Massie, B. and Kamen A. Serum-free production of recombinant proteins and adenoviral vectors by 293SF cells. Submitted for publication.
7. Couture, F., Lochmüller, H., Bouthillier, F., Li, G., Garnier, G., Petrof, B. J., Larochelle, N., Allen, C., Karpati, G. and Massie, B. Monitoring GFP expression as a new method for quantifying adenoviral vector mediated gene transfer. Submitted for publication.
8. Crameri, A.; Whitehorn, E. A.; Tate, E.; Stemmer, W. P. C. Improve green fluorescent protein by molecular evolution using DNA snuffling. Nat. Biotechnol. 1996, 14, 315-319.
9. Garnier, A.; Côté, J.; Nadeau, I.; Kamen, A.; Massie, B. Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells. Cytotechnology 1994, 15, 145-155.
10. Graham, F. L. Growth of 293 cells in suspension culture. J. Gen.Virol. 1987, 68, 937-940.
11. Graham, F. L.; Smiley, J.; Russel, W. C.; Nairn, R. Characteristics of human cell line transformed by DNA from human adenovirus 5. J. Gen. Virol. 1977, 36, 59-72.
12. Heim, R.; Cubitt, A. B.; Tsien, R. Y. Improved green fluorescence. Nature 1995, 373, 663-664.
13. Massie, B.; Dionne, J.; Lamarche, N.; Fleurent, J.; Langelier, Y. Improved adenovirus vector produces herpes simplex virus ribonucleotide reductase R1 and R2 subunits very efficiently. Bio/Technology 1995, 13, 602-608.
14. Mittereder, N.; March, K. L.; Trapnell, B. C. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J. Virol. 1996, 70, 7498-7509.
15. Mosser, D. D.; Caron, A. W.; Bourget, L.; Jolicoeur, P.; Massie, B. Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. BioTechniques 1997, 22, 150-161.
16. Nadeau, I.; Gamier, A.; Côté, J.; Massie, B.; Chavarie, C.; Kamen, A. Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies. Biotechnol. Bioeng. 1996, 51, 613-623.
17. Persson, R.; Svensson, U.; Everitt, E. Virus receptor in the adenovirus system. II Caping and cooperative binding of virions on HeLa cells. J. Virol. 1983, 46, 956-963.
18. Peshwa, M. V.; Kyung, Y.-S.; McClure, D. B.; Hu, W.-S. Cultivation of mammalian cells as aggregates in bioreactors: effect of calcium concentration on spatial distribution of viability. Biotechnol. Bioeng. 1993, 41, 179-187.
19. Ragot, T.; Finerty, S.; Watkins, P. E.; Perricaudet, M.; Morgan, A. J. Replication-defective recombinant adenovirus expressing the Epstein-Barr virus (EBV) envelope glycoprotein gp340/ 220 induces protective immunity against EBV-induced lymphomas in the cottontop tamarin. J. Gen. Virol. 1993a, 74, 501-507.
20. Ragot, T.; Vincent, N.; Chafey, V.; Vigne, E.; Gildenkrantz, H.; Couton, D.; Cartaud J.; Briand, P.; Kaplan, J.-C.; Perricaudet, M.; Kahn, A. Efficient adenovirus-mediated transfer of a human minidystrophin gene to skeletal muscle of mdx mice. Nature 1993b, 361, 647-650.
21. Shabram, P. Are adenovirus particles mostly infective or non-infective? Williamsburg Bioprocessing Conference, 3rd annual meeting proceedings, Virginia, Nov 18-21, 1996.
22. Stearns, T. The green revolution. Curr. Biol. 1995, 5, 3.
23. Yokoe, H. And Meyer, T. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nat. Biotechnol. 1996, 14, 1252-1256.
24. Zabner, J.; Couture, L. A.; Gregory, R. J.; Graham, S. M.; Smith, A. E.; Welsh, M. J. Adenovirus-mediated gene transfer transiently corrects the chloride transport defect in nasal epithelia of patients with cystic fibrosis. Cell 1993, 75, 207-216.
25. Zhai, Y.; Yang, J. C.; Kawakami, Y.; Spiess, P.; Wadsworth, S. C.; Cardoza, L. M.; Couture, L. A.; Smith, A. E.; Rosenberg, A. Antigen-specific tumor vaccines-Development and characterization of recombinant adenoviruses encoding MART1 or gp100 for cancer therapy. J. Immunol. 1996, 156, 700-710.
26. Zolotukhin, S.; Potter, M.; Hauswirth, W. W.; Guy, J.; Muzyczka, N. A "Immunized" green fluorescent protein cDNA adapted for high-expression in mammalian cells. J. Virol. 1996, 70, 4646-4654.