A novel autoregulated proliferation-controlled production process using recombinant CHO cells

Biotechnology and Bioengineering

Volumn 65, Issue 2, 1999, Pages 144-150

  Mazur, Xenia ^a   Eppenberger, Hans M ^a   Bailey, James E ^a   Fussenegger, Martin ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Bioprocesses; Heterologous protein; Proliferation

Indexed keywords

ANIMAL CELL CULTURE; BIODEGRADATION; GROWTH KINETICS; PROTEINS;

HETEROLOGOUS PROTEINS; TWO-PHASE BIOPROCESSES;

GENETIC ENGINEERING;

DOXYCYCLINE; TETRACYCLINE;

ANIMAL CELL; ARTICLE; AUTOMATION; CELL PROLIFERATION; CHO CELL; GENETIC LINKAGE; GROWTH INHIBITION; NONHUMAN; PROMOTER REGION; PROTEIN PROCESSING;

ANIMALS; CELL CYCLE PROTEINS; CELL DIVISION; CHO CELLS; CRICETINAE; CYCLIN-DEPENDENT KINASE INHIBITOR P27; DOXYCYCLINE; ENZYME INHIBITORS; FLOW CYTOMETRY; MICROTUBULE-ASSOCIATED PROTEINS; MUTATION; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; TETRACYCLINE; TUMOR SUPPRESSOR PROTEINS;

ANIMALIA;

EID: 0033589374     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19991020)65:2<144::AID-BIT3>3.0.CO;2-Q     Document Type: Article

References (53)

1. Ackland-Berglund CE, Leib DA. 1995. Efficiency of tetracycline-controlled gene expression is influenced by cell type. BioTechniques 18:196-200.
2. Baim SB, Labow MA, Levine AJ, Shenk T. 1991. A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-D-thiogalactopyranoside. Proc Natl Acad Sci USA 89:5072-5076.
3. Bebbington CR, Renner G, Thomson S, King D, Abbrams D, Yarranton GT. 1992. High level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio/Technology 10:169-175.
4. Chen CL, Gu X. 1995. Determination of tetracycline residues in bovine, serum and urine by capillary electrophoresis. J AOAC Int 78: 1369-1377.
5. Cocket MI, Bebbington CR, Yarranton GT. 1990. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification. Bio/Technology 8: 662-667.
6. Cooke SJ, Coates K, Barton CH, Biggs TE, Barrett SJ, Cochrane A, Oliver K, McKeating JA, Harris MP, Mann DA. 1997. Regulated expression vectors demonstrate cell-type-specific sensitivity to human immuno-deficiency virus type 1 Nef-induced cytostasis. J Gen Virol 78: 381-392.
7. Couture ML, Heath CA. 1995. Relationship between loss of heavy chains and the appearance of nonproducing hybridomas. Biotechnol Bioeng 47:270-275.
8. Croubels S, Vermeersch H, De Backer P, Santos MDF, Remon JP, Van Peteghem C. 1998. Liquid Chromatographic separation of doxycycline and 4-epidoxycycline in a tissue depletion study of doxycycline in turkeys. J Chromotogr B 708:145-152.
9. Day ST, Crouthamel WG, Martinelli LC, Ma JKH. 1978. Mechanism of fluorometric analysis of tetracycline involving metal complexation. J Pharm Sci 67:1518-1523.
10. Dhawan J, Rando TA, Elson SL, Bujard H, Blau HM. 1995. Tetracycline-regulated gene expression following direct gene transfer into mouse skeletal muscle. Som Cell Molec Genet 21:233-240.
11. Freundlieb S, Baron U, Bonin AL, Gossen M, Bujard H. 1997. Use of tetracycline-controlled gene expression systems to study mammalian cell cycle. Meth Enzymol 283:159-173.
12. Fussenegger M, Bailey JE. 1998. Molecular regulation of cell-cycle progression and apoptosis in mammalian cells: implications for biotechnology. Biotechnol Prog 14:807-833.
13. Fussenegger M, Bailey JE, Hauser H, Müller PP. 1998a. Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends Biotechnol 17:35-42.
14. Fussenegger M, Mazur X, Bailey JE. 1997a. A novel cytostatic process enhances the productivity of CHO cells. Biotechnol Bioeng 55: 927-939.
15. Fussenegger M, Mazur X, Bailey JE. 1998b. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells. Biotechnol Bioeng 57:1-10.
16. Fussenegger M, Moser S, Mazur X, Bailey JE. 1997b. Autoregulated multicistronic expression vectors provide one-step cloning of regulated product gene expression in mammalian cells. Biotechnol Prog 13: 733-740.
17. Fussenegger M, Schlatter S, Dätwyler D, Mazur X, Bailey JE. 1998c. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nature Biotechnol 16:468-472.
18. Gallia GL, Khalili K. 1998. Evaluation of an autoregulatory tetracycline regulated system. Oncogene 16:1879-1884.
19. Goldman MH, James DC, Ison AP, Bull AT. 1997. Monitoring proteolysis of recombinant human interferon-γ during batch culture of CHO cells. Cytotechnology 23:103-111.
20. Gossen M, Bujard H. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci 89: 5547-5551.
21. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, Bujard H. 1995. Transcriptional activation by terracycline in mammalian cells. Science 268:1766-1769.
22. Gu H, Marth JD, Orban PC, Mossman H, Rajewsky K. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265:103-106.
23. Gu MB, Todd P, Kompala DS. 1993. Foreign gene expression (β-galactosidase) during the cell-cycle phases in recombinant CHO cells. Biotechnol Bioeng 42:1113-1123.
24. Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strange PG, Bull AT. 1991. Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture. Appl Microbiol Biotechnol 34:559-564.
25. Hayter PM, Kirkby NF, Spier RE. 1992. Relationship between hybridoma growth and monoclonal antibody production. Enz Microbial Technol 14:454-461.
26. Hoffmann A, Villalba M, Journot L, Spengler D. 1997. A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cells. Nucl Acids Res 25:1078-1079.
27. Kaufman H, Mazur X, Fussenegger M, Bailey JE. 1999. Influence of low temperature on productivity, proteome and protein phosphorylation of CHO-cells. Biotechnol Bioeng 63:573-582.
28. Königbauer MJ, Brooks SH, Rullo G, Couch RA. 1992. Solid-state stability testing of drugs by isothermal calorimetry. Pharm Res 9:939-944.
29. Lee KH, Sburlati A, Renner WA, Bailey JE. 1996. Deregulated expression of cloned transcription factor E2F-1 in Chinese hamster ovary cells shifts protein patterns and activates growth in protein-free medium. Biotechnol Bioeng 50:273-279.
30. Leelavatcharamas V, Emery AN, Al-Rubeai M. 1994. Growth and interferon-γ production in batch culture of CHO cells. Cytotechnology 15:65-71.
31. Mazur X, Fussenegger M, Renner WA, Bailey EJ. 1998. Higher productivity of growth-arrested CHO cells expressing the cyclin-dependent kinase inhibitor p27. Biotechnol Prog 14:705-713.
32. Miller RF, Sokoloski TD, Mitscher LA, Bonacci AC, Hoener B. 1973. Use of circular dichroism in analysis of mixtures of tetracycline and 4-epitetracycline and its application to assay of commercial products. J Pharm Sci 62:1143-1147.
33. Mitchell CA, Beall JA, Wells JRE, Gray PP. 1991. Growth and protein production kinetics of a murine myeloma cell line transfected with the human growth hormone gene. Cytotechnology 5:223-231.
34. Morrison CJ, McMaster WR, Piret JM. 1997. Differential stability of proteolytically active and inactive recombinant metalloproteinase in CHO cells. Biotechnol Bioeng 53:594-600.
35. Munzert E, Muthing J, Buntemeyer H, Lehmann J. 1996. Sialidase activity in culture fluid of Chinese hamster ovary cells during batch culture and its effects on recombinant human antithrombin III integrity. Biotechnol Prog 12:559-563.
36. No D, Yao TP, Evans RM. 1996. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci USA 93: 3346-3351.
37. Ogata M, Marumoto Y, Oh IK, Shimizu S, Katoh S. 1994. Continuous culture of CHO-K1 cells producing thrombomodulin and estimation of culture conditions. J Ferment Bioeng 77: 46-51.
38. Paulus W, Baur I, Boyce FM, Breakefield XO, Reeves SA. 1996. Self-contained, tetracycline-regulated retroviral vector system for gene delivery to mammalian cells. J Virol 70:62-67.
39. Pendse GJ, Karkare S, Bailey JE. 1992. Effect of cloned gene dosage on cell growth and hepatitis B surface antigen in recombinant CHO cells. Biotechnol Bioeng 40:119-129.
40. Pikaart MJ, Recillas-Targa R, Felsenfeld G. 1998. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and his-tone deacetylation and is prevented by insulators. Genes Dev 12: 2852-2862.
41. Renner WA, Lee KH, Hatzimanikatis V, Bailey JE, Eppenberger HH. 1995. Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium. Biotechnol Bioeng 47:476-482.
42. Reynisdóettir I, Polyak K, lavarone A, Massagué J. 1995. Kip/Cip and Ink4 Cdk inhibitors cooperate to induce cell cycle arrest in response to TGF-β. Genes Dev 9:1831-1845.
43. Robinson DK, Memmert KW. 1991. Kinetics of recombinant inununoglobulin production by mammalian cells in continuous culture. Biotechnol Bioeng 38:972-976.
44. Schimke RT. 1984. Gene amplification in cultured animal cells. Cell 37: 705-713.
45. Seewoester T, Lehmann J. 1997. Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells. Biotechnol Bioeng 55:793-797.
46. Shocket P, Difilippantonio M, Hellman N, Schatz DG. 1995. A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc Natl Acad Sci USA 92:6522-6526.
47. Smiley AL, Hu WS, Wang DIC. 1989. Production of human immune interferon by recombinant mammalian cells cultivated on microcarriers. Biotechnol Bioeng 33: 1182-1190.
48. Sokoloski TD, Mitscher LA, Yuen PH, Juvarkar JV, Hoener B. 1977. Rate and proposed mechanism of anhydrotetracycline epimerization in acid solution. J Pharm Sci 66:1159-1165.
49. Tonouchi N, Koyama N, Miwa K. 1992. A CHO strain producing high-level human II-6 with the 3′ deletion construct. J Biotechnol 22: 2833-2839.
50. Vienneau DS, Kindberg CG. 1997. Development and validation of a sensitive method for tetracycline in gingival crevicular fluid by HPLC using fluorescence detection. J Pharm Biomed Anal 16:111-117.
51. Wu SY, Chiang CM. 1996. Establishment of stable cell lines expressing potentially toxic proteins by tetracycline-regulated and epitopetagging methods. BioTechniques 21:718-725.
52. Zang M, Trautmann H, Gandor C, Messi F, Asselbergs F, Leist C, Fiechter A, Reiser J. 1995. Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium. Bio/ Technology 13:389-3392.
53. Zhang Y, Katakura Y, Ohashi H, Shirahata S. 1997. Efficient and inducible production of human interleukin 6 in Chinese hamster ovary cells using a novel expression system. Cytotechnology 25:53-60.