The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition

Journal of Cell Biology

Volumn 156, Issue 2, 2002, Pages 249-259

  Kang, Dongmin ^a   Chen, James ^a   Wong, Jim ^a   Fang, Guowei ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

Cdc2; Chfr; Mitosis; Plk1; Ubiquitin protein ligase

Indexed keywords

CELL ENZYME; CELL PROTEIN; LIGASE; POLO LIKE KINASE 1; PROTEIN CHFR; PROTEIN KINASE; UNCLASSIFIED DRUG; WEE1 KINASE;

ARTICLE; CELL CYCLE G2 PHASE; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; MITOSIS INHIBITION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; STRESS; XENOPUS;

ANIMALS; BLOTTING, WESTERN; CDC2 PROTEIN KINASE; CELL CYCLE PROTEINS; CELL LINE; CELL-FREE SYSTEM; ENZYME ACTIVATION; G2 PHASE; HUMANS; LIGASES; MATURATION-PROMOTING FACTOR; MITOSIS; NEOPLASM PROTEINS; PHOSPHORYLATION; PHOSPHOTYROSINE; PROTEIN KINASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; UBIQUITIN; XENOPUS; XENOPUS PROTEINS;

EID: 0037148527     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200108016     Document Type: Article

References (48)

1. SUMO-1 modification of Mdm2 prevents its self-ubiquitination and increases Mdm2 ability to ubiquitinate p53
2. Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate
3. Cell cycle checkpoints-preventing an identity crisis
4. Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1
5. The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphasepromoting complex to control anaphase initiation
6. Mitotic Proteolysis
7. Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53
8. Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase
9. Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs
10. Cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2
11. The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex
12. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis
13. The ubiquitin system
14. The inhibitor of apoptosis, cIAP2, functions as a ubiquitin-protein ligase and promotes in vitro monoubiquitination of caspases 3 and 7
15. Elimination of cdc2 phosphorylation sites in the cdc25 phosphatase blocks initiation of M-phase
16. Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity
17. The lore of the RINGs: Substrate recognition and catalysis by ubiquitin ligases
18. Ubiquitin-conjugating enzymes: Novel regulators of eukaryotic cells
19. Cell cycle arrest by Colcemid differs in human normal and tumor cells
20. RING finger proteins: Mediators of ubiquitin ligase activity
21. The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase
22. Mitosis in transition
23. How proteolysis drives the cell cycle
24. Membrane localization of the kinase which phosphorylates p34cdc2 on rhreonine 14
25. Proteolysis and the GI-S transition: The SCF connection
26. The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system
27. Regulation of the cdc25 protein during the cell cycle in Xenopus extracts
28. Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts
29. Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes
30. The APC11 RING-H2 finger mediates E2-dependent ubiquitination
31. Polo encodes a protein kinase homolog required for mitosis in Drosophila
32. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination
33. Myt1: A membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15
34. Cyclin synthesis drives the early embryonic cell cycle
35. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity
36. Parallel activation of the NIMA and p34cdc2 cell cycle-regulated protein kinases is required to initiate mitosis in A. nidulans
37. Mitotic and G2 checkpoint control: Regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on setine-216
38. Cdc25+ functions as an inducer in the mitotic control of fission yeast
39. Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog
40. Conservation of the Chk1 checkpoint pathway in mammals: Linkage of DNA damage to Cdk regulation through Cdc25
41. Chfr defines a mitotic stress checkpoint that delays entry into metaphase
42. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34
43. Cyclin activation of p34cdc2
44. Ubiquitin-protein ligase activity of X-linked inhibitor of apoptosis protein promotes proteasomal degradation of caspase-3 and enhances its anti-apoptotic effect in Fas-induced cell death
45. SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis
46. Ligand-induced ubiquitination of the epidermal growth factor receptor involves the interaction of the c-Cbl RING finger and UbcH7
47. Src-catalyzed phosphorylation of c-Cbl leads to the interdependent ubiquitination of both proteins
48. Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases