The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases

EMBO Journal

Volumn 21, Issue 17, 2002, Pages 4699-4708

  Liu, Hudan ^a   Rodgers, Nancy D ^a   Jiao, Xinfu ^a   Kiledjian, Megerditch ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

Decapping; HIT motif; Mammalian mRNA turnover

Indexed keywords

EXONUCLEASE; HEXAPEPTIDE; INORGANIC PYROPHOSPHATASE; MESSENGER RNA; PROTEIN DCPS; SCAVENGER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME DEGRADATION; HUMAN; HUMAN CELL; HYDROLYSIS; HYDROPHOBICITY; MAMMAL CELL; MOUSE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PURIFICATION; RNA METABOLISM; RNA SEQUENCE; RNA STRUCTURE; SEQUENCE ANALYSIS;

AMINO ACID MOTIFS; CAENORHABDITIS ELEGANS PROTEINS; DNA, COMPLEMENTARY; DROSOPHILA PROTEINS; ENDORIBONUCLEASES; HUMANS; METHYLATION; MOLECULAR WEIGHT; MULTIGENE FAMILY; PYROPHOSPHATASES; RECOMBINANT FUSION PROTEINS; RNA CAPS; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY;

ANIMALIA; MAMMALIA;

EID: 0037009517     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf448     Document Type: Article

References (53)

1. Stereochemical retention of the configuration in the action of Fhit on phosphorus-chiral substrates
2. The 3′ to 5′ degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3′ to 5′ exonucleases of the exosome complex
3. An essential component of the decapping enzyme required for normal rates of mRNA turnover
4. Poly(A), poly(A) binding protein and the regulation of mRNA stability
5. Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins
6. The histidine triad superfamily of nucleotide-binding proteins
7. Characterization of c-myc 3′ to 5′ mRNA decay activities in an in vitro system
8. A cis-acting element known to block 3′ mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant
9. The yin and yang of the exosome
10. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs
11. Messenger RNA deadenylylation precedes decapping in mammalian cells
12. The exosome: A versatile RNA processing machine
13. A turnover pathway for both stable and unstable mRNAs in yeast: Evidence for a requirement for deadenylation
14. Cap-dependent deadenylation of mRNA
15. RNA delivery in Saccharomyces cerevisiae using electroporation
16. ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system
17. An mRNA surveillance mechanism that eliminates transcripts lacking termination codons
18. Interaction between a poly(A)-specific ribonuclease and the 5′ cap influences mRNA deadenylation rates in vitro
19. Regulation of mRNA stability in mammalian cells
20. Specific and nonspecific enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphates
21. Dinucleoside 5′, 5‴-P1, P3-triphosphate hydrolase from yellow lupin (Lupinus luteus) seeds: Purification to homogeneity and hydrolysis of mRNA 5′-cap analogs
22. Yeast cells lacking 5′→3′ exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5′ cap structure
23. The 3′ to 5′ degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3′ to 5′ exonucleases of the exosome complex
24. Diadenosine oligophosphates (Ap(n)A), a novel class of signalling molecules?
25. The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes
26. Purification and properties of a decapping enzyme from rat liver cytosol
27. Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme
28. Structure-based analysis of catalysis and substrate definition in the HIT protein family
29. A 54-kDa fragment of the poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting poly(A)-specific 3′ exonuclease
30. The exosome: A conserved eukaryotic RNA processing complex containing multiple 3′→5′ exoribonucleases
31. Premature translational termination triggers mRNA decapping
32. Turnover mechanisms of the stable yeast PGK1 mRNA
33. The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements
34. Characterization of the m7G(5′)pppN-pyrophosphatase activity from HeLa cells
35. Detection in HeLa cell extracts of a 7-methyl guanosine specific enzyme activity that cleaves m7GpppNm
36. Genetic, biochemical, and crystallographic characterization of Fhit-substrate complexes as the active signaling form of Fhit
37. Characterization and purification of a mammalian endoribonuclease specific for the α-globin mRNA
38. H4 histone messenger RNA decay in cell-free extracts initiates at or near the 3′ terminus and proceeds 3′ to 5′
39. In vitro folding of inclusion body proteins
40. Protein synthesis assayed by electroporation of mRNA in Saccharomyces cerevisiae
41. The HIT protein family: A new family of proteins present in prokaryotes, yeast and mammals
42. Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay
43. Generation of stable mRNA fragments and translation of N-truncated proteins induced by antisense oligodeoxynucleotides
44. Yeast exosome mutants accumulate 3′-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs
45. Exosome-mediated recognition and degradation of mRNAs lacking a termination codon
46. Identification of an erythroid-enriched endoribonuclease activity involved in specific mRNA cleavage
47. Functional link between the mammalian exosome and mRNA decapping
48. An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro
49. The hDcp2 protein is a mammalian mRNA decapping enzyme
50. Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3′ AU-rich sequences
51. The cap-to-tail guide to mRNA turnover
52. + hematopoietic stem/progenitor cells
53. Monitoring mRNA decapping activity