Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme

EMBO Journal

Volumn 17, Issue 5, 1998, Pages 1487-1496

  LaGrandeur, Thomas E ^a   Parker, Roy ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

Decapping; Decay; mRNA; Turnover; Yeast

Indexed keywords

MESSENGER RNA; METHYL GROUP; PHOSPHOPROTEIN;

ADENYLATION; ARTICLE; DNA DEGRADATION; ENZYME ISOLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; RNA CAPPING; YEAST;

ENDORIBONUCLEASES; FUNGAL PROTEINS; PHOSPHOGLYCERATE KINASE; PHOSPHORYLATION; RECOMBINANT FUSION PROTEINS; RNA CAPS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, FUNGAL; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0032473359     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.5.1487     Document Type: Article

References (42)

1. Adams,B.L., Morgan,M., Muthukrishnan,S., Hecht,S.M. and Shatkin,A.J. (1978) The effect of cap analogs on reovirus mRNA binding to wheat germ ribosomes. J. Biol. Chem., 253, 2589-2595.
2. Bashkirov,V.I., Scherthan,H., Solinger,J.A., Buerstedde,J.-M. and Heyer,W.-D. (1997) A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates. J. Cell Biol., 136, 761-773.
3. Beelman,C.A. and Parker,R. (1995) Degradation of mRNA in eukaryotes. Cell, 81, 179-183.
4. Beelman,C.A., Stevens,A., Caponigro,G., LaGrandeur,T.E., Hatfield,L., Fortner,D.M. and Parker,R. (1996) An essential component of the decapping enzyme required for normal rates of mRNA turnover. Nature, 382, 642-646.
5. Blank,A., Sugiyama,R.H. and Dekker,C.A. (1982) Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels. Anal. Biochem., 120, 267-275.
6. Caponigro,G. and Parker,R. (1995) Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast. Genes Dev., 9, 2421-2432.
7. Cereghino,G.P. and Scheffler,I.E. (1995) Glucose-dependent turnover of the mRNAs encoding succinate dehydrogenase peptides in Saccharomyces cerevisiae: sequence elements in the 5′ untranslated region of the Ip mRNA play a dominant role. Mol. Biol. Cell, 6, 1125-1143.
8. Couttet,P., Fromont-Racine,M., Steel,D., Pictet,R. and Grange,T. (1997) Messenger RNA deadenylation precedes decapping in mammalian cells. Proc. Natl Acad. Sci. USA, 94, 5628-5633.
9. Cui,Y., Hagan,K.W., Zhang,S. and Peltz,S.W. (1995) Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translation termination codon. Genes Dev., 9, 423-436.
10. Decker,C.J. and Parker,R. (1993) A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation. Genes Dev., 7, 1632-1643.
11. Gonzalez,C.I. and Martin,C.E. (1996) Fatty acid-responsive control of mRNA stability. J. Biol. Chem., 271, 25801-25809.
12. Hagan,K.W., Ruiz-Echevarria,M.J., Quan,Y. and Peltz,S.W. (1995) Characterization of cu-acting sequences and decay intermediates involved in nonsense-mediated mRNA turnover. Mol. Cell. Biol., 15, 809-823.
13. Harris,M.E., Nolan,J.M., Malhotra,A., Brown,J.W., Harvey,S.C. and Pace,N.R. (1994) Use of photoaffinity crosslinking and molecular modeling to analyze the global architecture of ribonuclease P RNA. EMBO J., 13, 3953-3963.
14. Hatfield,L., Beelman,C.A., Stevens,A. and Parker,R. (1996) Mutations in trans-acting factors affecting mRNA decapping in Saccharomyces cerevisiae. Mol. Cell. Biol., 16, 5830-5838.
15. He,F. and Jacobson,A. (1995) Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen. Genes Dev., 9, 437-454.
16. Heaton,B., Decker,C., Muhlrad,D., Donahue,J., Jacobson,A. and Parker,R. (1992) Analysis of chimeric mRNAs derived from the STE3 mRNA identifies multiple regions within yeast mRNAs that modulate mRNA decay. Nucleic Acids Res., 20, 5365-5373.
17. Heyer,W.D., Johnson,A.W., Reinhart,U. and Kolodner,R.D. (1995) Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease. Mol. Biol. Cell, 15, 2728-2736.
18. Higgs,D.C. and Colbert,J.T. (1994) Oat phytochrome A mRNA degradation appears to occur via two distinct pathways. Plant Cell, 6, 1007-1019.
19. Hodel,A., Shi,X. and Gershon,P.D. (1997) Specific protein recognition of an mRNA cap through its alkylated base. Nature Struct. Biol., 4, 350-354.
20. Konarska,M.M., Padgett,R.A. and Sharp,P.A. (1984) Recognition of cap structure in splicing in vitro of mRNA precursors. Cell, 38, 731-736.
21. Laemmli,U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
22. LaGrandeur,T.E., Hüttenhofer,A., Noller,H.F and Pace,N.R. (1994) Phylogenetic comparative chemical footprint analysis of the interaction between ribonuclease P RNA and tRNA. EMBO J., 13, 3945-3952.
23. Leeds,P., Peltz,S.W., Jacobson,A. and Culbertson,M.R. (1991) The product of the yeast UPF1 gene is required for rapid turnover of mRNAs containing a premature translational termination codon. Genes Dev., 5, 2303-2314.
24. Leeds,P., Wood,J.M., Lee,B.-S. and Culbertson,M.R. (1992) Gene products that promote mRNA turnover in Saccharomyces cerevisiae. Mol. Cell. Biol., 12, 2165-2177.
25. Lim,S.K. and Maquat,L.E. (1992) Human β-globin mRNAs that harbor a nonsense codon are degraded in murine erythroid tissues to intermediates lacking regions of exon I or exons I and II that have a cap-like structure at the 5′ termini. EMBO J., 11, 3271-3278.
26. Marcotrigianol,J., Gingras,A.-C., Sonenberg,N., and Burley,S.K. (1997) Cocrystal structure of the messenger RNA 5′ cap-binding protein (eIF-4E) bound to 7-methyl GDP. Cell, 89, 951-961.
27. McCubbin,W.D., Edery,I., Altmann,M., Sonenberg,N. and Kay,C.M. (1988) Circular dichroism and fluorescence studies on protein synthesis initiation factor eIF-4E and two mutant forms from the yeast Saccharomyces cerevisiae. J. Biol. Chem., 263, 17663-17671.
28. Merrick,W.C. and Hershey,J.W.B. (1996) The pathway and mechanism of eukaryotic protein synthesis. In Hershey,J.W.B., Mathews,M.B. and Sonenberg,N. (eds), Translational Control. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Milligan,J.F. and Uhlenbeck,O.C. (1989) Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol., 180, 51-62.
30. Muhlrad,D. and Parker,R. (1992) Mutations affecting stability and deadenylation of the yeast MFA2 transcript. Genes Dev., 6, 2100-2111.
31. Muhlrad,D. and Parker,R. (1994) Premature translational termination triggers mRNA decapping. Nature, 370, 578-581.
32. Muhlrad,D., Decker,C.J. and Parker,R. (1994) Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5′ to 3′ digestion of the transcript. Genes Dev., 8, 855-866.
33. Muhlrad,D., Decker,C.J. and Parker,R. (1995) Turnover mechanisms of the stable yeast PGK1 mRNA. Mol. Cell. Biol., 15, 2145-2156.
34. Pierrat,B., Lacroute,F. and Losson,R. (1993) The 5′ untranslated region of the PPR1 regulatory gene dictates rapid mRNA decay in yeast. Gene, 131, 43-51.
35. Rubin,G.M. (1973) The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J. Biol. Chem., 248, 3860-3875.
36. Schena,M., Picard,D. and Yamamoto,K.R. (1991) Vectors for constitutive and inducible gene expression in yeast. Methods Enzymol., 194, 389-398.
37. Schnierle,B-S., Gershon,P.D. and Moss,B. (1992) Cap-specific mRNA (nucleoside-O2′)-methyltransferase and poly(A) polymerase stimulatory activities of vaccinia virus are mediated by a single protein. Proc. Natl Acad. Sci. USA, 89, 2897-2901.
38. 2)-methyltransferase and 3′- adenylyltransferase stimulatory activities encoded by vaccinia virus. J. Biol. Chem., 269, 20700-20708.
39. Shi,X., Yao,P., Jose,T. and Gershon,P.D. (1996) Methyltransferasespecific domains within VP-39, a bifunctional protein which participates in the modification of both mRNA ends. RNA, 2, 88-101.
40. Shuman,S. and Moss,B. (1990) Purification and use of vaccinia virus messenger RNA capping enzyme. Methods Enzymol., 181, 170-180.
41. Stevens,A. (1988) mRNA-decapping enzyme from Saccharomyces cerevisiae: purification and unique specificity for long RNA chains. Mol Cell Biol., 8, 2005-2010.
42. Yanisch-Perron,C., Vieira,J. and Messing,J. (1985) Improved m13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103-119.