Quantitation of the P2Y1 receptor with a high affinity radiolabeled antagonist

Molecular Pharmacology

Volumn 62, Issue 5, 2002, Pages 1249-1257

  Waldo, Gary L ^a   Corbitt, James ^a   Boyer, José L ^a   Ravi, Gnana ^b   Kim, Hak Sung ^b   Ji, Xiao Duo ^b   Lacy, James ^c   Jacobson, Kenneth A ^b   Kendall Harden, T ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c PerkinElmer Life Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 CHLORO N6 METHYLMETHANOCARBA 2' DEOXYADENOSINE 3',5' BISPHOSPHATE; ION CHANNEL; METABOTROPIC RECEPTOR; MRS 2279; PURINE P2Y RECEPTOR; RECEPTOR SUBTYPE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BRAIN MEMBRANE; COMPETITIVE INHIBITION; EXTRACELLULAR MATRIX; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RECEPTOR AFFINITY; RECEPTOR BINDING; SIGNAL TRANSDUCTION; THROMBOCYTE AGGREGATION;

ADENOSINE DIPHOSPHATE; ANIMALS; CELLS, CULTURED; FEMALE; HUMANS; INSECTS; RADIOPHARMACEUTICALS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, PURINERGIC P2; TRITIUM;

EID: 0036841643     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.62.5.1249     Document Type: Article

References (35)

1. Akbar GKM, Dasari VR, Webb TE, Ayyanathan K, Pillarisetti K, Sandhu AK, Athwal RS, Daniel JL, Ashby B, Barnard EA, et al. (1996) Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells. J Biol Chem 271:18363-18367.
2. 1 receptor and NTPDasel exhibits functional activities of the native receptor and ectoenzyme and reduced signaling responses to endogenously released nucleotides. Mol Pharmacol 62:521-528.
3. Baurand A, Raboisson P, Freund M, Leon C, Cazenave JP, Bourguignon JJ, and Gachet C (2001) Inhibition of platelet function by administration of MRS2179, a P2Y1 receptor antagonist. Eur J Pharmacol 412:213-221.
4. 6-methyl-(N)-methanocarba-2′-deoxyadenosine-3′, 5′-bisphosphate is a selective high affinity P2Y1 receptor antagonist. Br J Pharmacol 135:2004-2010.
5. 6-methyl 2′-deoxyadenosine 3′,5′-bisphosphate. Br J Pharmacol 124:1-3.
6. 1 receptor. Mol Pharmacol 50:1323-1329.
7. 1 receptor: Pharmacological, signaling and molecular properties. Drug Dev Res 39:253-261.
8. 2Y purinergic receptors. Br J Pharmacol 113:614-620.
9. 1 receptors. J Med Chem 41:183-190.
10. 2-purinergic receptor. J Biol Chem 264:6202-6206.
11. 2-purinergic receptors for extracellular ATP and other nucleotides. Am J Physiol 265:C577-C606.
12. Harden TK, Barnard EA, Boeynaems JM, Burnstock G, Dubyak GR, Hourani SMO, and Insel PA (1998a) P2Y receptors, in The IUPHAR Compendium of Receptor Characterization and Classification (Girdlestone D ed) pp 209-217, IUPHAR Media, London.
13. 2-purinergic receptors: Subtype-associated signaling responses and structure. Annu Rev Pharmacol Toxicol 35:541-579.
14. Harden TK, Lazarowski ER, and Boucher RC (1997) Release, metabolism and interconversion of adenine and uridine nucleotides: Implications for G protein-coupled P2 receptor agonist selectivity. Trends Pharmacol Sci 18:43-46.
15. Harden TK, Nicholas RA, Schachter JB, Lazarowski ER, and Boyer JL (1998b) Pharmacological selectivities of molecularly defined subtypes of P2Y receptors, in The P2 Nucleotide Receptors (Turner JT, Weisman GA, and Fedan JS eds) pp 109-134, Humana Press, Totowa, NJ.
16. Hechler B, Vigne P, Leon C, Breittmayer JP, and Gachet C (1998) ATP derivatives are antagonists of the P2Y1 receptor: Similarities to the platelet ADP receptor. Mol Pharmacol 53:727-733.
17. Huang P, Lazarowski ER, Tarran R, Milgram SL, Boucher RC, and Stutts MJ (2001) Compartmentalized autocrine signaling to cystic fibrosis transmembrane conductance regulator at the apical membrane of airway epithelial cells. Proc Natl Acad Sci USA 98:14120-14125.
18. Khakh BS, Barnard EA, Burnstock G, Kennedy C, King BF, North RA, Seguela P, Voigt M, and Humphrey PPA (2000) P2X receptors, in The IUPHAR Compendium of Receptor Characterization and Classification (Girdlestone D ed) pp 291-305, IUPHAR Media, London.
19. Kim HS, Barak D, Harden TK, Boyer JL, and Jacobson KA (2001) Acyclic and cyclopropyl analogues of adenosine bisphosphate antagonists of the P2Y1 receptor: Structure-activity relationships and receptor docking. J Med Chem 44:3092-3108.
20. Kim Y-C, Gallo-Rodriguez C, Jang S-Y, Nandanan E, Adams M, Harden TK, Boyer JL, and Jacobson KA (2000) Acyclic analogues of deoxyadenosine 3′, 5′-bisphosphates as P2Y1 receptor antagonists. J Med Chem 42:746-755.
21. Motte S, Swillens S, and Boeynaems JM (1996) Evidence that most high-affinity ATP binding sites on aortic endothelial cells and membranes do not correspond to P2 receptors. Eur J Pharmacol 307:201-209.
22. Nandanan E, Camaioni E, Jang SY, Kim YC, Cristalli G, Herdewijn P, Secrist JA 3rd, Tiwari KN, Mohanram A, Harden TK, et al. (1999) Structure-activity relationships of bisphosphate nucleotide derivatives as P2Y1 receptor antagonists and partial agonists. J Med Chem 42:1625-1638.
23. 1 receptor ligands. J Med Chem 43:829-842.
24. 1 receptor. Mol Pharmacol 54:1118-1123.
25. Perich JW and Johns RB (1988) Di-tert-butyl N,N-niethylphosphoramidite. A new phosphorylating agent for the efficient phosphorylation of alcohols. Synthesis 2:142-144.
26. Ralevic V and Burnstock G (1998) Receptors for purines and pyrimidines. Pharmacol Rev 50:413-492.
27. 1 receptor. Br J Pharmacol 121:338-344.
28. 1 receptor. Br J Pharmacol 118:167-173.
29. Simon J, Vigne P, Eklund KM, Michel AD, Carruthers AM, Humphrey PP, Frelin C, and Barnard EA (2001) Activity of adenosine diphosphates and triphosphates on a P2Y(T)-type receptor in brain capillary endothelial cells. Br J Pharmacol 132:173-182.
30. Simon J, Webb TE, and Barnard EA (1995a) Characterization of a P2Y purinoceptor in the brain. Pharmacol Toxicol 76:302-307.
31. 35S]dATPαS binding sites in the adult rat neuraxis. Neuropharmacology 36:1243-1251.
32. Simon J, Webb TE, King BF, Burnstock G, and Barnard EA (1995b) Characterization of a recombinant P2Y purinoceptor. Eur J Pharmacol 291:281-289.
33. 5. Biochem Biophys Res Commun 219:105-110.
34. 35S]2′-Deoxy 5′-O-(1-thio) ATP binding sites and the P2Y1 messenger RNA within the chick brain. Neuroscience 84:825-837.
35. Zimmermann H (1996) Extracellular purine metabolism. Drug Dev Res 39:337-352.