Optimisation of real-time quantitative RT-PCR for the evaluation of non-viral mediated gene transfer to the airways

Gene Therapy

Volumn 9, Issue 19, 2002, Pages 1312-1320

  Rose, A C ^a,d   Goddard, C A ^b   Colledge, W H ^b   Cheng, S H ^c   Gill, D R ^a   Hyde, S C ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c GENZYME CORPORATION   (United States)

^d JOHNSON AND JOHNSON PHARMACEUTICAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

GL 67; Lung cystic fibrosis; Non viral gene transfer; Quantitative RT PCR; TaqMan

Indexed keywords

ION; LIPOSOME; MESSENGER RNA; NAKED DNA; PLASMID DNA; PLASMID VECTOR; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; EVALUATION; FEMALE; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER; HUMAN; HUMAN CELL; ION TRANSPORT; LUNG FIBROSIS; MEDICAL TECHNOLOGY; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RESPIRATORY SYSTEM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSGENE;

ANIMALS; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; FEMALE; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; LIPOSOMES; MICE; MICE, INBRED BALB C; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSGENES;

ANIMALIA; MURINAE;

EID: 0036791579     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301792     Document Type: Article

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