Recent advances in in vitro molecular directed evolution

Progress in Biochemistry and Biophysics

Volumn 29, Issue 4, 2002, Pages 518-522

  Xu, Hui Fang ^a   Zhang, Xian En ^a   Zhang, Yong Mei ^a   Cass, A E G ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

Author keywords

DNA shuffling; Genomic evolution; In vitro directed evolution; ITCHY; Protein engneering

Indexed keywords

DNA;

ANALYTIC METHOD; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; ERROR; IN VITRO STUDY; METHODOLOGY; MUTAGENESIS; POLYMERASE CHAIN REACTION; REVIEW; SCREENING; TECHNOLOGY;

EID: 0036674642     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (18)

1. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening
2. Enzyme engineering for nonaqueous solvent: Random mutagenesis to enhance activity of sublisin E in polar organic media
3. Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
4. Rapid evolution of a protein in vitro by DNA shuffling
5. Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of improved sequences
6. DNA shuffling of a family of genes from diverse species accelerates evolution
7. Molecular evolution by staggered extension process (StEP) in vitro recombination
8. Random-priming in vitro recombination: An effective tool for directed evolution
9. DNA shuffling method for generating highly recombined genes and evolved enzymes
10. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast
11. A combinatorial approach to hybrid enzymes independent of DNA homology
12. Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides
13. Libraries of hybrid proteins from distantly related sequence
14. In vitro display technologies: Novel developments and applications
15. Novel concepts for selection of catalytic activity
16. High-throughput screening: New technology for the 21st century
17. Directed evolution of polymerase function by compartmentalized self-replication
18. Directed evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: A labratory model of molecular evolution