Transfection technology and the study of drug resistance in the malaria parasite Plasmodium falciparum

Drug Resistance Updates

Volumn 5, Issue 3-4, 2002, Pages 126-130

  Crabb, Brendan S ^a  

^a ROYAL MELBOURNE HOSPITAL   (Australia)

Author keywords

Drug resistance; Plasmodiumfalciparum; Transfection

Indexed keywords

4,6 DIAMINO 1,2 DIHYDRO 2,2 DIMETHYL 1 [3 (2,4,5 TRICHLOROPHENOXY)PROPOXY] 1,3,5 TRIAZINE;

ANTIBIOTIC RESISTANCE; MALARIA; MICROBIOLOGICAL EXAMINATION; MOLECULAR GENETICS; MUTATION; NONHUMAN; PHENOTYPE; PLASMODIUM FALCIPARUM; POINT MUTATION; PRIORITY JOURNAL; SHORT SURVEY; TECHNOLOGY; TRANSGENE;

ANIMALS; DRUG RESISTANCE; MOLECULAR BIOLOGY; PHENOTYPE; PLASMODIUM FALCIPARUM; TRANSFECTION;

EID: 0036618855     PISSN: 13687646     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1368-7646(02)00085-7     Document Type: Short Survey

References (23)

1. RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum
2. A set of independent selectable markers for transfection of the human malaria parasite Plasmodiumfalciparum
3. A P-glycoprotein homologue of Plasmodium falciparum is localized on the digestive vacuole
4. Selection for mefloquine resistance in Plasmodium falciparum is linked to amplification of the pfmdr1 gene and cross-resistance to halofantrine and quinine
5. Characterization of promoters and stable transfection by homologous and non-homologous recombination in Plasmodium falciparum
6. Targeted gene disruption shows that knobs enable malaria-infected red cells to cytoadhere under physiological shear stress
7. Puromycin-N-acetyl-transferase as a selectable marker for use in Plasmodium falciparum
8. Stable molecular transformation of Toxoplasma gondii: A selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria
9. Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for hit-and-run mutagenesis
10. Negative selection of Plasmodium falciparum reveals targeted gene deletion by double crossover recombination
11. Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil
12. Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance
13. Allelic modifications of the cg2 and cg1 genes do not alter the chloroquine response of drug-resistant Plasmodium falciparum
14. Several alleles of the multidrug-resistance gene are closely linked to chloroquine resistance in Plasmodium falciparum
15. Disruption of the Pfg27 locus by homolgous recombination leads to loss of the sexual phenotype in Plasmodium falciparum
16. Functional conservation of the malaria vaccine antigen MSP-119 across distantly related Plasmodium species
17. An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites
18. A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodiumfalciparum chromosomes
19. Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum
20. Complex polymorphisms in an approximately 330 kDa protein are linked to chloroquine-resistant P falciparum in Southeast Asia and Africa
21. Allelic exchange at the endogenous genomic locus in Plasmodium falciparum proves the role of dihydropteroate synthase in sulfadoxine-resistant malaria
22. Chloroquine resistance not linked to mdr-like genes in a Plasmodium falciparum cross
23. Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine