Studies on the mechanism of stabilization of partially phosphorothioated oligonucleotides against nucleolytic degradation

Antisense and Nucleic Acid Drug Development

Volumn 7, Issue 4, 1997, Pages 345-350

  Uhlmann, Eugen ^a   Ryte, Antonina ^a,b   Peyman, Anusch ^a  

^a SANOFI   (France)

^b GEORGETOWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE; OLIGODEOXYNUCLEOTIDE DERIVATIVE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; OLIGODEOXYRIBONUCLEOSIDE METHYLPHOSPHONATE;

ARTICLE; CATTLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; DNA DEGRADATION; DNA STRUCTURE; NONHUMAN; PRIORITY JOURNAL; SERUM; SIDE EFFECT;

EID: 0030830147     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1997.7.345     Document Type: Article

References (5)

1. CROOKE, R.M., GRAHAM, M.J., COOKE, M.E., and CROOKE, S.T. (1995). In vitro pharmacokinetics of phosphorothioate antisense oligonucleotides. J. Pharmacol. Exp. Ther. 275, 462-473.
2. FENNEWALD, S.M., and RANDO, R.F. (1995). Inhibition of high affinity basic fibroblast growth factor binding by oligonucleotides. J. Biol. Chem. 270, 21718-21721.
3. GAO, W.Y., HANES, R.N., VAZQUEZ, P.M.A., STEIN, C.A., COHEN, J.S., and CHENG, Y.C. (1990a). Inhibition of herpes simplex virus type 2 growth by phosphorothioate oligodeoxynucleotides. Antimicrob. Agents Chemother. 34, 808-812.
4. GAO, W.Y., JAROSZEWSKI, J.W., COHEN, J.S., and CHENG, Y.C. (1990b). Mechanisms of inhibition of herpes simplex virus type 2 growth by 28-mer phosphorothioate oligodeoxycytidine. J. Biol. Chem. 265, 20172-20178.
5. YU, D., IYER, R.P., SHAW, D.R., LISZIEWICZ, J., LI, Y., JIANG, Z., ROSKEY, A., and AGRAWAL, S. (1996). Hybrid oligonucleotides: Synthesis, biophysical properties, stability studies, and biological activity. Bioorg. Med. Chem. 4, 1685-1692.