Stopped-flow kinetics of locked nucleic acid (LNA) - Oligonucleotide duplex formation: Studies of LNA-DNA and DNA-DNA interactions

Biochemical Journal

Volumn 354, Issue 3, 2001, Pages 481-484

  Christensen, U ^a   Jacobsen, N ^a   Rajwanshi, V K ^a   Wengel, J ^a   Koch, T ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

DNA oligomer; Off rates; On rates; Thermodynamics

Indexed keywords

ABSORPTION; CHEMICAL REACTIONS; DISSOCIATION; DNA; MONOMERS; RNA; THERMODYNAMIC STABILITY; THERMODYNAMICS;

LOCKED NUCLEIC ACID (LNA); OLIGONUCLEOTIDES;

NUCLEIC ACIDS;

DNA; MONOMER; NUCLEOTIDE DERIVATIVE; OLIGONUCLEOTIDE;

ARTICLE; COMPLEX FORMATION; DISSOCIATION CONSTANT; DNA BINDING; KINETICS; PRIORITY JOURNAL; THERMODYNAMICS;

DNA; KINETICS; MACROMOLECULAR SUBSTANCES; MODELS, THEORETICAL; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDES; THERMODYNAMICS;

KOBUS;

EID: 0035868358     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3540481     Document Type: Article

References (16)

1. LNA (locked nucleic acids): Synthesis and high-affinity nucleic acid recognition
2. LNA (Locked Nucleic Acid): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, ologomerisation, and unprecedented nucleic acid recognition
3. Synthesis of 3′-C- and 4′-C-branched oligonucleotides and the development of locked nucleic acid (LNA)
4. Stability and structural features of the duplexes containing nucleoside analogues with a N-type conformation, 2′-O,4′-C-methyleneribonucleosides
5. Detection of the factor V Leiden mutation by direct allele-specific hybridization of PCR amplicons to photo-immobilized locked nucleic acids
6. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules
7. Peptide nucleic acids (PNA): Synthesis, properties and potential applications
8. 1′,5′-Anhydrohexitol oligonucleotides: Synthesis, base pairing and recognition by regular oligodeoxyribonucleotides and oligoribonucleotides
9. 1′,5′-Anhydrohexitol oligonucleotides: Hybridization and strand displacement with oligoribonucleotides, interaction with RNase H and HIV reverse transcriptase
10. Oligo-2′-fluoro-2′-deoxynucleotide N3′-P5′ phosphoramidates: Synthesis and properties
11. Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
12. Modern machine-aided methods of oligodeoxyribonucleotide synthesis
13. Kinetics of renaturation of DNA
14. Structural studies of LNA: DNA duplxes by NMR: Conformations and implications for RNase activity
15. Base-stacking and base-pairing contributions to helix stability: Thermodynamics of double-helix formation with CCGG, CCGGp, CCCAp, ACCGGp, CCGGUp, and ACCGGUp
16. LNA (locked nucleic acid): An RNA mimic forming exceedingly stable LNA:LNA duplexes