Design of antisense oligonucleotides stabilized by locked nucleic acids

Nucleic Acids Research

Volumn 30, Issue 9, 2002, Pages 1911-1918

  Kurreck, Jens ^a   Wyszko, Eliza ^a,b   Gillen, Clemens ^c   Erdmann, Volker A ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b INSTITUTE OF BIOORGANIC CHEMISTRY   (Poland)

^c GRÜNENTHAL GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; DNA; FOMIVIRSEN; MONOMER; NUCLEIC ACID; NUCLEOTIDE; PHOSPHOROTHIOIC ACID DERIVATIVE; RIBONUCLEASE H;

ARTICLE; CHIMERA; CONTROLLED STUDY; DRUG APPROVAL; DRUG DESIGN; DRUG HALF LIFE; DRUG STABILITY; ENZYME ACTIVATION; MEDICAL LITERATURE; MELTING POINT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA CLEAVAGE; SERUM; TEMPERATURE;

EID: 0036567147     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/30.9.1911     Document Type: Article

References (32)

1. Synthetic oligonucleotides: Useful molecules? A review
2. Progress in antisense technology: The end of the beginning
3. Basic principles of using antisense oligonucleotides in vivo
4. Antisense oligonucleotides: Promise and reality
5. First "antisense" drug will treat CMV retinitis
6. Effects of phosphorothioate modification of oligodeoxynucleotides on specific protein binding
7. Phosphorothioate oligodeoxynucleotides bind to basic fibroblast growth factor, inhibit its binding to cell surface receptors and remove it from low affinity binding sites on extracellular matrix
8. Cell-surface pertubations of the epidermal growth factor and vascular endothelial growth factor receptors by phosphorothioate oligodeoxynucleotides
9. A review of issues in the pharmacokinetics and toxicology of phosphorothioate antisense oligonucleotides
10. Mixed-backbone oligonucleotides as second generation antisense oligonucleotides: In vitro and in vivo studies
11. New candidates for true antisense
12. A critical survey of the structure-function of the antisense oligo/RNA heteroduplex as substrate for RNase H
13. Cleavage of single strand RNA adjecent to RNA-DNA duplex regions by Escherichia coli RNase H1
14. Kinetic characteristics of Escherichia coli RNase H1: Cleavage of various antisense oligonucleotide-RNA duplexes
15. Evaluation of 2′-modified oligonucleotides containing 2′-deoxy gaps as antisense inhibitors of gene expression
16. Properties of cloned and expressed human RNase H1
17. Cyclohexene nucleic acids (CeNA): Serum stable oligonucleotides that activate RNase H and increase duplex stability with complementary RNA
18. RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA)
19. Locked nucleic acid (LNA): Fine-tuning the recognition of DNA and RNA
20. Application of PNA and LNA oligomers to chemotherapy
21. LNA (locked nucleic acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation and unprecendented nucleic acid recognition
22. Novel convenient syntheses of LNA [2.2.1] bicyclo nucleosides
23. Stability and structural features of the duplexes containing nucleoside analogues with fixed N-type conformation, 2′-O,4′-C-methyleneribonucleosides
24. Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
25. A capsaicin-receptor homologue with a high threshold for noxious heat
26. The cloned capsaicin receptor integrates multiple pain-producing stimuli
27. Comparative study of DNA enzymes and ribozymes against the same full length messenger RNA of the vanilloid receptor subtype I
28. Structural studies of LNA:RNA duplexes by NMR: Conformations and implications for RNase H activity
29. Nucleotides LXIV[1]: Synthesis, hybridization and enzymatic degradation studies of 2′-O-methyl-oligoribonucleotides and 2′-O-methyl/deoxy gapmers
30. Oligonucleotide analogue interference with the HIV-1 TAT protein-TAR RNA interaction
31. Oligodeoxynucleoside phosphotothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluid
32. Minimally modified oligonucleotides-combination of end-capping and pyrimidine-protection